The dataset captures media-dependent physicochemical properties and biological responses in in vitro lung models. Hydrodynamic diameter and PDI are measured via DLS at t₀ (immediately after preparation) and t₂₄ (24 hours later), with SD values reported. Exposure conditions include concentration (expressed as µg/mL), and time of exposure (h). Biological features include cell line, cell type, cell origin, and well format (e.g., 96-well plate). Cell viability is evaluated using Alamar Blue assays along with inflammation-ROS quantification, and genotoxicity (γH2AX).

The dataset captures exposure conditions and cellular responses, of NM-induced cytotoxicity (WST-1), ROS quantification (DHR123, CellROX) and genotoxicity (53BP1, micronucleus chromosomal damage in binucleated cells using High-Content Analysis). It includes NM solution treatment (e.g., sonication), culture medium (e.g., DMEM), exposure concentration, and duration (h)

The environmental ε_Bioaccumulation.xlsx dataset provides bioaccumulation Ti uptake in biological organisms, following OECD 317 protocol. Enchytraeus crypticus were exposed to TiO₂ in soil for 14 days to monitor uptake rates (by mass), followed by a 14-day transfer to clean soil to assess elimination. The nominal exposure concentration (mg/kg) represents the intended TiO2 NM dose administered in the soil. The dataset captures sampling phase, and time points post-exposure, supporting toxicokinetic analysis. The data differentiates between pooled vs. individual samples by indicated the number of organisms included in the sample, and includes biomass measurements (wet and dry weight at each sampling time point).

From the exposure campaigns (10.5281/zenodo.16641460) the alveolar retained doses (μg/cm²) from Multiple-path particle dosimetry (MPPD) model (10.5281/zenodo.16642495) were then translated into real occupational exposure doses and tested in Tier 2 advanced in vitro models, using A549 cells co-cultured with alveolar macrophages derived from THP-1 monocytes (Motta et al. 2024). σ_Ηuman lung_real dose.xlsx dataset includes physicochemical properties, and biological responses of NM (not NEP_ID), supporting hazard evaluation. Biological responses assess NM effects on the cell lines under exposure conditions (ng/cm², h). Cytotoxicity is measured via lactate dehydrogenase (LDH) release (%), while inflammatory responses are evaluated through fold changes in pro-inflammatory cytokines, including Interleukins 8, 6 and 1β (IL-8, IL-6, IL-1β).