The σ_Acellular.xlsx dataset captures experimental conditions for assessing NM-induced oxidative stress. The reactive molecule (e.g., glutathione (GSH), reactive nitrogen/oxygen species (RNO)) and media type define the reaction environment. The physicochemical properties include hydrodynamic diameter (DLS) and ζ-potential (ELS), with pH recorded. The properties reported in this dataset are not duplicates of those provided in the dedicated physicochemical files. Instead, they represent separate measurements conducted by a different partner, under different conditions, and often in exposure-relevant media. As a result, they should be interpreted as complementary and not redundant. Exposure conditions cover concentration, duration, and intermediate preparation volumes. The dataset records control absorbance, peak absorbance wavelength, and unreacted model molecule concentration. The percentage of the model molecule and GSH/RNO consumed provides a measure of NM-induced redox activity.

The dataset focuses on hydrodynamics and surface charge properties. It includes the initial stock concentration (weight-based) and the diluted working concentration. Hydrodynamic diameter and PDI are measured using DLS in suspension, with SDs reported. Surface charge properties are assessed through ζ-potential measurements in water, recorded alongside SDs and pH values, using ELS. The isoelectric point is reported, indicating the pH at which NMs exhibit zero net surface charge. This dataset is complete, with no missing values.

The environmental ε_Bioaccumulation.xlsx dataset provides bioaccumulation Ti uptake in biological organisms, following OECD 317 protocol. Enchytraeus crypticus were exposed to TiO₂ in soil for 14 days to monitor uptake rates (by mass), followed by a 14-day transfer to clean soil to assess elimination. The nominal exposure concentration (mg/kg) represents the intended TiO2 NM dose administered in the soil. The dataset captures sampling phase, and time points post-exposure, supporting toxicokinetic analysis. The data differentiates between pooled vs. individual samples by indicated the number of organisms included in the sample, and includes biomass measurements (wet and dry weight at each sampling time point).