This statistic depicts the average body mass index (BMI) of U.S. adults aged 20 years and over as of 2016, by gender. According to the data, the average male BMI has increased from 27.8 in 1999-2000 to 29.1 as of 2015-2016.

This statistic depicts the average body mass index (BMI) of U.S. females aged 20 years and over from 1999 to 2016, by ethnicity. According to the data, the average female BMI for those that identified as white was **** in 1999-2000 and increased to **** as of 2015-2016.

United States Prevalence of Overweight: % of Adults data was reported at 67.900 % in 2016. This records an increase from the previous number of 67.400 % for 2015. United States Prevalence of Overweight: % of Adults data is updated yearly, averaging 55.200 % from Dec 1975 (Median) to 2016, with 42 observations. The data reached an all-time high of 67.900 % in 2016 and a record low of 41.000 % in 1975. United States Prevalence of Overweight: % of Adults data remains active status in CEIC and is reported by World Bank. The data is categorized under Global Database’s United States – Table US.World Bank.WDI: Social: Health Statistics. Prevalence of overweight adults is the percentage of adults ages 18 and over whose Body Mass Index (BMI) is more than 25 kg/m2. Body Mass Index (BMI) is a simple index of weight-for-height, or the weight in kilograms divided by the square of the height in meters.;World Health Organization, Global Health Observatory Data Repository (http://apps.who.int/ghodata/).;;

Surveys in which U.S. adults report their current weight have shown that the share of those reporting they weigh 200 pounds or more has increased over the past few decades. In 2024, around 28 percent of respondents reported their weight as 200 pounds or more, compared to 15 percent in 1990. However, the same surveys show the share of respondents who report they are overweight has decreased compared to figures from 1990. What percentage of the U.S. population is obese? Obesity is an increasing problem in the United States that is expected to become worse in the coming decades. As of 2023, around one third of adults in the United States were considered obese. Obesity is slightly more prevalent among women in the United States, and rates of obesity differ greatly by region and state. For example, in West Virginia, around 41 percent of adults are obese, compared to 25 percent in Colorado. However, although Colorado is the state with the lowest prevalence of obesity among adults, a quarter of the adult population being obese is still shockingly high. The health impacts of being obese Obesity increases the risk of developing a number of health conditions including high blood pressure, heart disease, type 2 diabetes, and certain types of cancer. It is no coincidence that the states with the highest rates of hypertension are also among the states with the highest prevalence of obesity. West Virginia currently has the third highest rate of hypertension in the U.S. with 45 percent of adults with the condition. It is also no coincidence that as rates of obesity in the United States have increased so have rates of diabetes. As of 2022, around 8.4 percent of adults in the United States had been diagnosed with diabetes, compared to six percent in the year 2000. Obesity can be prevented through a healthy diet and regular exercise, which also increases overall health and longevity.

United States US: Prevalence of Overweight: Weight for Height: Female: % of Children Under 5 data was reported at 6.900 % in 2012. This records an increase from the previous number of 6.400 % for 2009. United States US: Prevalence of Overweight: Weight for Height: Female: % of Children Under 5 data is updated yearly, averaging 6.900 % from Dec 1991 (Median) to 2012, with 6 observations. The data reached an all-time high of 8.700 % in 2005 and a record low of 5.100 % in 1991. United States US: Prevalence of Overweight: Weight for Height: Female: % of Children Under 5 data remains active status in CEIC and is reported by World Bank. The data is categorized under Global Database’s USA – Table US.World Bank: Health Statistics. Prevalence of overweight, female, is the percentage of girls under age 5 whose weight for height is more than two standard deviations above the median for the international reference population of the corresponding age as established by the WHO's new child growth standards released in 2006.; ; World Health Organization, Global Database on Child Growth and Malnutrition. Country-level data are unadjusted data from national surveys, and thus may not be comparable across countries.; Linear mixed-effect model estimates; Estimates of overweight children are also from national survey data. Once considered only a high-income economy problem, overweight children have become a growing concern in developing countries. Research shows an association between childhood obesity and a high prevalence of diabetes, respiratory disease, high blood pressure, and psychosocial and orthopedic disorders (de Onis and Blössner 2003). Childhood obesity is associated with a higher chance of obesity, premature death, and disability in adulthood. In addition to increased future risks, obese children experience breathing difficulties and increased risk of fractures, hypertension, early markers of cardiovascular disease, insulin resistance, and psychological effects. Children in low- and middle-income countries are more vulnerable to inadequate nutrition before birth and in infancy and early childhood. Many of these children are exposed to high-fat, high-sugar, high-salt, calorie-dense, micronutrient-poor foods, which tend be lower in cost than more nutritious foods. These dietary patterns, in conjunction with low levels of physical activity, result in sharp increases in childhood obesity, while under-nutrition continues

Data from a clustered randomized controlled trial of 12 Head Start Centers in San Antonio, Texas: !Míranos! Look at Us, We Are Healthy! (¡Míranos!)The prevalence of obesity remains high in American children aged 2-5 while one in three Head Start children is overweight or obese. The !Míranos! study was designed to test the efficacy of !Míranos!, an early childhood obesity prevention program, which promoted healthy growth in predominantly Latino children in Head Start. The Míranos! included center-based (policy changes, staff development, gross motor program, and nutrition education) and home-based (parent engagement/ education and home visits) interventions to address key enablers and barriers in obesity prevention in young children. In partnership with Head Start, the study team demonstrated the feasibility and acceptability of the proposed interventions to influence energy-balance-related behaviors favorably in Head Start children. Using a three-arm cluster randomized design, 21 Head Start centers in equal numbers wiere randomly assigned to one of three conditions: 1) a combined center- and home-based intervention, 2) center-based intervention only, or 3) control. The interventions were delivered during the academic year (an 8-month period). A total of 526 3-year-old children were enrolled in the study and followed prospectively one year post-intervention. Outcome data collection was conducted at baseline, immediate post-intervention, and 1-year follow-up and included height, weight, physical activity (PA), and sedentary behaviors by accelerometry, parent reports of sleep duration and TV watching time, gross motor development, dietary intakes, and food and activity preferences. Information on family background, parental weight, PA- and nutrition-related practices and behaviors, PA and nutrition policy and environment at center and home, intervention program costs, and treatment fidelity will also be collected. The study had three specific aims: 1) to test the efficacy of the !Míranos! intervention on healthy weight growth (primary outcome) in normal weight, overweight and obese children, 2) to test the impact of the !Míranos! intervention on children’s PA, sedentary behavior, sleep, and dietary behaviors (secondary outcomes), and 3) to evaluate the cost-effectiveness of the !Míranos! intervention. By targeting different levels of influence and in multiple settings, the !Míranos! showed great promise of developing long-term health habits that reduce the energy imbalance gap by targeting multiple energy-balance-related behaviors. The !Míranos! can be disseminated to various organized childcare settings since it is built on the Head Start program and its infrastructure—a gold standard in early childhood education, as well as current PA and nutrition recommendations for preschool children.

BackgroundFew studies have examined weight transitions in contemporary multi-ethnic populations spanning early childhood through adulthood despite the ability of such research to inform obesity prevention, control, and disparities reduction.Methods and ResultsWe characterized the ages at which African American, Caucasian, and Mexican American populations transitioned to overweight and obesity using contemporary and nationally representative cross-sectional National Health and Nutrition Examination Survey data (n = 21,220; aged 2–80 years). Age-, sex-, and race/ethnic-specific one-year net transition probabilities between body mass index-classified normal weight, overweight, and obesity were estimated using calibrated and validated Markov-type models that accommodated complex sampling. At age two, the obesity prevalence ranged from 7.3% in Caucasian males to 16.1% in Mexican American males. For all populations, estimated one-year overweight to obesity net transition probabilities peaked at age two and were highest for Mexican American males and African American females, for whom a net 12.3% (95% CI: 7.6%-17.0%) and 11.9% (95% CI: 8.5%-15.3%) of the overweight populations transitioned to obesity by age three, respectively. However, extrapolation to the 2010 U.S. population demonstrated that Mexican American males were the only population for whom net increases in obesity peaked during early childhood; age-specific net increases in obesity were approximately constant through the second decade of life for African Americans and Mexican American females and peaked at age 20 for Caucasians.ConclusionsAfrican American and Mexican American populations shoulder elevated rates of many obesity-associated chronic diseases and disparities in early transitions to obesity could further increase these inequalities if left unaddressed.

West Virginia, Mississippi, and Arkansas are the U.S. states with the highest percentage of their population who are obese. The states with the lowest percentage of their population who are obese include Colorado, Hawaii, and Massachusetts. Obesity in the United States Obesity is a growing problem in many countries around the world, but the United States has the highest rate of obesity among all OECD countries. The prevalence of obesity in the United States has risen steadily over the previous two decades, with no signs of declining. Obesity in the U.S. is more common among women than men, and overweight and obesity rates are higher among African Americans than any other race or ethnicity. Causes and health impacts Obesity is most commonly the result of a combination of poor diet, overeating, physical inactivity, and a genetic susceptibility. Obesity is associated with various negative health impacts, including an increased risk of cardiovascular diseases, certain types of cancer, and diabetes type 2. As of 2022, around 8.4 percent of the U.S. population had been diagnosed with diabetes. Diabetes is currently the eighth leading cause of death in the United States.

Data on overweight and obesity among adults aged 20 and over in the United States, by selected characteristics, including sex, age, race, Hispanic origin, and poverty level. Data are from Health, United States. SOURCE: National Center for Health Statistics, National Health and Nutrition Examination Survey. Search, visualize, and download these and other estimates from over 120 health topics with the NCHS Data Query System (DQS), available from: https://www.cdc.gov/nchs/dataquery/index.htm.

United States US: Prevalence of Overweight: Weight for Height: % of Children Under 5 data was reported at 6.000 % in 2012. This records a decrease from the previous number of 7.800 % for 2009. United States US: Prevalence of Overweight: Weight for Height: % of Children Under 5 data is updated yearly, averaging 7.000 % from Dec 1991 (Median) to 2012, with 5 observations. The data reached an all-time high of 8.100 % in 2005 and a record low of 5.400 % in 1991. United States US: Prevalence of Overweight: Weight for Height: % of Children Under 5 data remains active status in CEIC and is reported by World Bank. The data is categorized under Global Database’s USA – Table US.World Bank: Health Statistics. Prevalence of overweight children is the percentage of children under age 5 whose weight for height is more than two standard deviations above the median for the international reference population of the corresponding age as established by the WHO's new child growth standards released in 2006.; ; UNICEF, WHO, World Bank: Joint child malnutrition estimates (JME). Aggregation is based on UNICEF, WHO, and the World Bank harmonized dataset (adjusted, comparable data) and methodology.; Linear mixed-effect model estimates; Estimates of overweight children are also from national survey data. Once considered only a high-income economy problem, overweight children have become a growing concern in developing countries. Research shows an association between childhood obesity and a high prevalence of diabetes, respiratory disease, high blood pressure, and psychosocial and orthopedic disorders (de Onis and Blössner 2003). Childhood obesity is associated with a higher chance of obesity, premature death, and disability in adulthood. In addition to increased future risks, obese children experience breathing difficulties and increased risk of fractures, hypertension, early markers of cardiovascular disease, insulin resistance, and psychological effects. Children in low- and middle-income countries are more vulnerable to inadequate nutrition before birth and in infancy and early childhood. Many of these children are exposed to high-fat, high-sugar, high-salt, calorie-dense, micronutrient-poor foods, which tend be lower in cost than more nutritious foods. These dietary patterns, in conjunction with low levels of physical activity, result in sharp increases in childhood obesity, while under-nutrition continues

BackgroundUnderstanding the social environmental around obesity has been limited by available data. One promising approach used to bridge similar gaps elsewhere is to use passively generated digital data.PurposeThis article explores the relationship between online social environment via web-based social networks and population obesity prevalence.MethodsWe performed a cross-sectional study using linear regression and cross validation to measure the relationship and predictive performance of user interests on the online social network Facebook to obesity prevalence in metros across the United States of America (USA) and neighborhoods within New York City (NYC). The outcomes, proportion of obese and/or overweight population in USA metros and NYC neighborhoods, were obtained via the Centers for Disease Control and Prevention Behavioral Risk Factor Surveillance and NYC EpiQuery systems. Predictors were geographically specific proportion of users with activity-related and sedentary-related interests on Facebook.ResultsHigher proportion of the population with activity-related interests on Facebook was associated with a significant 12.0% (95% Confidence Interval (CI) 11.9 to 12.1) lower predicted prevalence of obese and/or overweight people across USA metros and 7.2% (95% CI: 6.8 to 7.7) across NYC neighborhoods. Conversely, greater proportion of the population with interest in television was associated with higher prevalence of obese and/or overweight people of 3.9% (95% CI: 3.7 to 4.0) (USA) and 27.5% (95% CI: 27.1 to 27.9, significant) (NYC). For activity-interests and national obesity outcomes, the average root mean square prediction error from 10-fold cross validation was comparable to the average root mean square error of a model developed using the entire data set.ConclusionsActivity-related interests across the USA and sedentary-related interests across NYC were significantly associated with obesity prevalence. Further research is needed to understand how the online social environment relates to health outcomes and how it can be used to identify or target interventions.

This dataset tracks the updates made on the dataset "Normal weight, overweight, and obesity among adults aged 20 and over, by selected characteristics: United States" as a repository for previous versions of the data and metadata.

In 2024, around 40 percent of U.S. men reported weighing 200 pounds or more. This statistic shows the average self-reported weight among U.S. men from 1990 to 2024.

This dataset tracks the updates made on the dataset "DEV DQS Normal weight, overweight, and obesity among adults aged 20 and over, by selected characteristics: United States" as a repository for previous versions of the data and metadata.

In Mississippi, over ***** out of ten adults were reported to be either overweight or obese in 2018, making it the leading U.S. state that year. Other prominent states, in terms of overweight and obesity, included Arkansas in ******, Oklahoma in *******, and Louisiana in ***** place.

Corpulence per state

When it comes to obesity, specifically, percentages were still very high for certain states. Almost forty percent of West Virginia’s population was obese in 2018. Colorado, Hawaii, and California were some of the healthier states that year, with obesity rates between ** and ** percent. The average for the country itself stood at just over ** percent.

Obesity-related health problems

Being obese can lead to various health-related complications, such as diabetes and diseases of the heart. In 2017, almost ** people per 100,000 died of diabetes mellitus in the United States. In the same year, roughly *** per 100,000 Americans died of heart disease. While the number of deaths caused by heart disease has decreased significantly over the past sixty to seventy years, it is still one of the leading causes of death in the country.

Supplementary files for article Supplementary information files for Height and body-mass index trajectories of school-aged children and adolescents from 1985 to 2019 in 200 countries and territories: a pooled analysis of 2181 population-based studies with 65 million participants.
BackgroundComparable global data on health and nutrition of school-aged children and adolescents are scarce. We aimed to estimate age trajectories and time trends in mean height and mean body-mass index (BMI), which measures weight gain beyond what is expected from height gain, for school-aged children and adolescents.
MethodsFor this pooled analysis, we used a database of cardiometabolic risk factors collated by the Non-Communicable Disease Risk Factor Collaboration. We applied a Bayesian hierarchical model to estimate trends from 1985 to 2019 in mean height and mean BMI in 1-year age groups for ages 5–19 years. The model allowed for non-linear changes over time in mean height and mean BMI and for non-linear changes with age of children and adolescents, including periods of rapid growth during adolescence.
FindingsWe pooled data from 2181 population-based studies, with measurements of height and weight in 65 million participants in 200 countries and territories. In 2019, we estimated a difference of 20 cm or higher in mean height of 19-year-old adolescents between countries with the tallest populations (the Netherlands, Montenegro, Estonia, and Bosnia and Herzegovina for boys; and the Netherlands, Montenegro, Denmark, and Iceland for girls) and those with the shortest populations (Timor-Leste, Laos, Solomon Islands, and Papua New Guinea for boys; and Guatemala, Bangladesh, Nepal, and Timor-Leste for girls). In the same year, the difference between the highest mean BMI (in Pacific island countries, Kuwait, Bahrain, The Bahamas, Chile, the USA, and New Zealand for both boys and girls and in South Africa for girls) and lowest mean BMI (in India, Bangladesh, Timor-Leste, Ethiopia, and Chad for boys and girls; and in Japan and Romania for girls) was approximately 9–10 kg/m2. In some countries, children aged 5 years started with healthier height or BMI than the global median and, in some cases, as healthy as the best performing countries, but they became progressively less healthy compared with their comparators as they grew older by not growing as tall (eg, boys in Austria and Barbados, and girls in Belgium and Puerto Rico) or gaining too much weight for their height (eg, girls and boys in Kuwait, Bahrain, Fiji, Jamaica, and Mexico; and girls in South Africa and New Zealand). In other countries, growing children overtook the height of their comparators (eg, Latvia, Czech Republic, Morocco, and Iran) or curbed their weight gain (eg, Italy, France, and Croatia) in late childhood and adolescence. When changes in both height and BMI were considered, girls in South Korea, Vietnam, Saudi Arabia, Turkey, and some central Asian countries (eg, Armenia and Azerbaijan), and boys in central and western Europe (eg, Portugal, Denmark, Poland, and Montenegro) had the healthiest changes in anthropometric status over the past 3·5 decades because, compared with children and adolescents in other countries, they had a much larger gain in height than they did in BMI. The unhealthiest changes—gaining too little height, too much weight for their height compared with children in other countries, or both—occurred in many countries in sub-Saharan Africa, New Zealand, and the USA for boys and girls; in Malaysia and some Pacific island nations for boys; and in Mexico for girls.
InterpretationThe height and BMI trajectories over age and time of school-aged children and adolescents are highly variable across countries, which indicates heterogeneous nutritional quality and lifelong health advantages and risks.

BMI, body mass index; N, unweighted number; IQR, interquartile range.

Weight loss service providers confront intense competition in a highly saturated and concentrated business environment. Proprietary diet plans have existed for decades, with significant industry players established as far back as the early 1960s. Company performance is influenced by domestic population growth, favorable economic conditions and health factors (obesity rate, healthy eating index). Despite the favorable economic conditions following COVID-19, competition from substitutes has stolen consumers. Industry-wide revenue declined at a CAGR of 2.9% to $3.5 billion, falling 0.1% in 2024 alone. As a result, industry profit declined at a similar rate throughout 2024. COVID-19 spurred consolidation and concentration. Larger firms were able to weather the downturn, and smaller establishments exited. Additionally, COVID-19 changed consumers' attitudes toward remote communications. Even though weight loss services have been traditionally in-person, lockdowns changed attitudes. Platforms and mobile technology enabled consumers to engage with others in new novel ways, changing the need for in-person service. Going forward, the continued emphasis on digital platforms will amplify competitive advantages for the most prominent weight loss service providers, causing many more small-scale companies to exit. Yet, regions like the Southeast with a significant obese population will be better served by larger enterprises that can provide better analytics and sophisticated communication modalities. Dominant enterprises may expand, gaining new revenue streams through acquisitions to better meet out-of-market competition products and services (Fitbit, Smartphone apps, social media groups, VR chat rooms.) Nevertheless, revenue will decline at a CAGR of 0.4% to $3.4 billion through the end of 2029.

This dataset tracks the updates made on the dataset "DQS Normal weight, overweight, and obesity among adults aged 20 and over, by selected characteristics: United States" as a repository for previous versions of the data and metadata.

Maternal obesity has been associated with a higher risk of pregnancy-related complications in mothers and offspring; however, effective interventions have not yet been developed. We tested two common interventions, calorie restriction and pravastatin administration, during pregnancy in a rhesus macaque model with the hypothesis that these interventions would normalize metabolic dysregulation in pregnant mothers leading to an improvement in infant metabolic and cognitive/social development. A total of 19 obese mothers were assigned to either one of the two intervention groups (n=5 for calorie restriction; n=7 for pravastatin) or an obese control group (n=7) with no intervention, and maternal gestational samples and postnatal infant samples were compared with lean control mothers (n=6). Gestational calorie restriction normalized one-carbon metabolism dysregulation in obese mothers but altered energy metabolism in their offspring. Although administration of pravastatin during pregnancy tended to normalize blood cholesterol in the mothers, it potentially impacted the gut microbiome and kidney function of their offspring. In the offspring, both calorie restriction and pravastatin administration during pregnancy tended to normalize the activity of AMPK in the brain at 6 months, and while results of the Visual Paired-Comparison test, which measures infant recognition memory, were not significantly impacted by either of the interventions, gestational pravastatin administration, but not calorie restriction, tended to normalize anxiety assessed by the Human Intruder test. Although the two interventions tested in a non-human primate model led to some improvements in metabolism and/or infant brain development, negative impacts were also found in both mothers and infants. Our study emphasizes the importance of assessing gestational interventions for maternal obesity on both maternal and offspring long-term outcomes. Methods Study population Pregnant female rhesus macaques (Macaca mulatta) from an indoor breeding colony at the California National Primate Research Center with appropriate social behavior and previous successful pregnancies were enrolled. Animal handling was approved by the UC Davis Institutional Animal Care and Use Committee (IACUC) (#19299). A qualitative real-time PCR assay (Jimenez & Tarantal, 2003) was used to identify mothers with male fetuses to include in this study. Since obesity is defined as subjects with body fat above 30% for women, according to guidelines from the American Society of Bariatric Physicians, American Medical Association, and in some publications (Okorodudu et al., 2010; Shah & Braverman, 2012), a Body Condition Score (BCS) of 3.5 (32.8 % body fat on average (Summers et al., 2012)) was used as the cutoff. Therefore, mothers with BCS of 3.5 and above were categorized as obese. Obese mothers were randomly assigned to the Obese Control (OC) group, OR group (received calorie Restriction), or OP group (received Pravastatin). Mothers with BCS of 2.5 and below were assigned to the Lean Control (LC) group. The unbalanced sample size was because some mothers were removed from the analyses due to fetal deaths for unknown reasons, misidentification of a female fetus, different timing for study enrollment, or technical issues upon collecting samples. The number of animals was six for the LC, seven for the OC, five for the OR, and seven for the OP groups. Feeding, rearing, and interventions Adult female animals were provided monkey diet (High Protein Primate Diet Jumbo #5047; LabDiet, St. Louis, MO, USA) twice a day between 6–9 am and 1–3 pm. The calories were provided as 56% from carbohydrates, 30% from protein, and 13% from. Mothers in the LC, OC, and OP groups were fed nine biscuits twice a day once pregnancy was confirmed. Mothers in the OR group received a restricted supply of food once the pregnancy was detected and was maintained throughout pregnancy. The food restriction was set such that the average total weight increase would be 8% body weight from the last day before conception because the recommended total weight gain in the 2nd and 3rd trimesters is 5-9 kg for the average US woman with obesity who weighs 80 kg and is 1.6 m in height (Body Mass Index of 30), according to the Institute of Medicine 2009 guidelines (Institute of Medicine and National Research Council, 2009). During nursing of infants older than 4 months, all mothers were provided twelve biscuits. Fresh produce was provided biweekly, and water was provided ad libitum for all mothers. Mothers in the OP group were given pravastatin sodium (ApexBio Technology, Houston, TX, USA) at 20 mg/kg body weight prepared in a neutralized syrup (20 mg/mL sodium bicarbonate dissolved in a fruit-flavored syrup (Torani, San Leandro, CA, USA)) once a day from the time pregnancy was confirmed until delivery. The caloric value of the administration was made so as not to influence body weight or skew nutritional value of the diet among all treatment groups. Both interventions were applied only during gestation. Although most mothers were allowed to deliver naturally, cesarean delivery was performed for fetal indications when recommended by veterinarians (2 for each of the LC and OC groups, and 1 for the OP group). These mothers did not accept their infant following birth, so foster mothers were provided. Sample Collection and pre-processing prior to sample storage The animal caretakers and researchers who collected samples were blinded for group assignment by coding all animals by IDs. The collected biological samples were randomized by using random numbers and the group assignment was blinded during the data collection. Both mothers (during pregnancy) and infants were weighed every week. One day before sample collection, food was removed 30 min after the afternoon feeding, and biological samples were collected prior to the morning feeding. To collect biological samples, animals were anesthetized using 5–30 mg/kg ketamine or 5–8 mg/kg telazol. Both maternal and infant blood was collected using 5 mL lavender top (EDTA) tubes (Monoject, Cardinal Health, Dublin, OH, USA) and urine was collected from the bladder by ultrasound-guided transabdominal cystotomy using a 22-gauge needle and stored in a 15 mL Falcon tube. A placental sample was collected at GD150 transabdominally under ultrasound guidance using an 18-gauge needle attached to a sterile syringe. Sample processing was as previously described in (Hasegawa et al., 2022). Necropsy was conducted between 9:30 am–1:30 pm. First, infants at the age of PD180 were fasted and anesthetized with ketamine, and plasma and urine were collected. Then, euthanasia was performed with 120 mg/kg pentobarbital, followed by heparin injection, clamping of the descending aorta, and flushing with saline until clear. The kidney and brain (amygdala, hippocampus, hypothalamus, and prefrontal cortex) were collected, weighed, and immediately frozen on dry ice or liquid nitrogen to store at -80 °C until further analyses. Metabolite extraction and analysis by 1H NMR, and measurement of insulin, cholesterol, cytokine, and cortisol Detailed procedures were previously described (Hasegawa et al., 2022). Briefly, plasma and urine samples were filtered using Amicon Ultra Centrifugal Filter (3k molecular weight cutoff; Millipore, Billerica, MA, USA), and the supernatant was used for analysis. For both the placental and brain tissue samples, polar metabolites were extracted using our previously reported method (Hasegawa et al., 2020). A total of 180 μL of sample (tissue extract or filtered urine or serum) was transferred to 3 mm Bruker NMR tubes (Bruker, Billerica, MA, USA). Within 24 h of sample preparation, all 1H NMR spectra were acquired using the noesypr1d pulse sequence on a Bruker Avance 600 MHz NMR spectrometer (Bruker, Billerica, MA, USA) (O’Sullivan et al., 2013). Chenomx NMRSuite (version 8.1, Chenomx Inc., Edmonton, Canada) (Weljie et al., 2006) was used to identify and quantify metabolites. Heparin-treated plasma samples were used to measure insulin and 17 cytokines and chemokines (hs-CRP, Granulocyte-macrophage colony-stimulating factor, IFN-γ, TNF-α, transforming growth factor-α, monocyte chemoattractant protein-1, macrophage inflammatory protein-1β (MIP-1β), and interleukin (IL)-1β, IL-1 receptor antagonist (IL-1ra), IL-2, IL-6, IL-8, IL-10, IL-12/23 p40, IL-13, IL-15, and IL-17A) using a multiplex Bead-Based Kit (Millipore) on a Bio-Plex 100 (Bio-rad, Hercules, CA) following the manufacturer’s protocol. For each sample, a minimum of fifty beads per region were collected and analyzed with Bio-Plex Manager software using a 5-point standard curve with immune marker quantities extrapolated based on the standard curve. Two samples were removed for analysis of TNF-α and IL-1ra as technical errors (both from Animal ID 1132103: 895.2 and 1115.1 pg/mL at gestational days (GD) 90; 510.8 and 617.2 pg/mL at GD120, respectively). Plasma cholesterol level was measured by Clinical Laboratory Diagnostic Product (OSR6116) on Beckman Coulter AU480 (Beckman Coulter, Brea, CA). Infant plasma cortisol level at PD110 was assessed as previously described (Vandeleest et al., 2019; Walker et al., 2018). In short, infants were transferred to a test room at 9 am and blood was drawn at 11 am (Sample 1), followed by another blood collection at 4 pm (Sample 2) and intramuscular injection of 500 μg/kg dexamethasone (Dex) (American Regent Laboratories, Inc., Shirley, NY). On the next day, a blood sample was collected at 8:30 am (Sample 3), and then 2.5 IU of adrenocorticotropic hormone (Amphastar Pharmaceuticals, Inc., Rancho Cucamonga, CA) was injected intramuscularly. The last blood was collected (Sample 4) 30 min after adrenocorticotropic hormone injection. The collected blood samples were processed and stored, and cortisol concentration was assessed by a chemiluminescent assay on the ADVIA Centaur CP platform