Data related to the publication Murschhauser et al.: A high-throughput microscopy method for single-cell analysis of event-time correlations in nanoparticle-induced cell death. It contains fluorescence time traces of single cells marked with cell-event markers and observed by time-lapse microscopy. The cells were treated with nanoparticles at different doses (NP25 and NP100), with staurosporine (sts) or were left untreated for control (ctrl). See the above-mentioned publication for more details.

The format of the data is described below.

The file Data_A549.zip contains data measured with A549 cells, and the file Data_Huh7.zip contains data measured with Huh7 cells. Both files have the same structure. Each file contains the directories Raw and Fitted as well as a checksum file. The Raw directory contains single-cell fluorescence time courses as obtained by time-lapse microscopy. The Fitted directory contains the results of fitting model functions as well as properties of identified events, such as event times. The checksum file contains SHA256 checksums of all files within these directories and can be used to check file integrity.

Both directories contain measurement directories. Each measurement directory contains the data corresponding to one experiment. The name of the measurement directory is the measurement identifier. Each measurement directory contains condition directories. Each condition directory contains data corresponding to one condition measured in the measurement and is named after the condition. Each condition directory contains marker directories. They are named after the fluorescence markers measured and contain files with single-cell data corresponding to the respective markers.

The names of those files consist of multiple parts separated by underscores. The first two parts identify a position of the microscope. Since pairs of markers were measured, each position is present in two marker directories. The third part is the measurement identifier. The other parts will be described below.

The Raw directory contains only CSV files with the raw fluorescence time courses. The filenames contain no other parts and have the suffix “.txt”. The first row of each CSV file is the time (in units of 10 minutes), and the other rows are the fluorescence time courses of the cells observed at the corresponding position (in arbitrary units). Each file in the Raw directory corresponds to a group of files in the Fitted directory.

The Fitted directory contains three types of CSV files. Their names have “ALL” as fourth part, a session identifier as sixth part and the suffix “.csv”. The fifth part indicates the type of file and is one of the following:

• “PARAMS” indicates the estimated values for the model parameters. Each row stands for one cell and each column for a parameter of the model function fitted to the data. The model functions are published with the fitting software.
• “SIMULATED” indicates the fitted traces. The traces are calculated using the model functions and the estimated parameters. The format is the same as for the raw traces, but the time is in units of hours and has a higher resolution.
• “STATE” indicates additional information extracted from the fitted traces. Each row stands for a cell and each column for a property. The first column is the number of the cell. The second column is the event time found (in hours); non-finite values indicate that no event time was found. The third and fourth columns contain the absolute and relative amplitude of the trace, respectively. The fifth column is the logarithmic likelihood of the best fit. The sixth column indicates an algorithm used for postprocessing, and the seventh column indicates the trace slope at the event. See the fitting software for details.