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Certain types of gene families, such as those encoding most families of transcription factors, maintain their chromosomal syntenic positions throughout Angiosperm evolutionary time. Other, non-syntenic gene families are prone to deletion, tandem duplication, and transposition. Here we describe the chromosomal positional history of all genes in Arabidopsis thaliana (A. thaliana) throughout the rosid superorder. We introduce a public database where researchers can look up the positional history of their favorite A. thaliana gene or gene family. Finally, we show that specific gene families transposed at specific points in evolutionary time, particularly after whole-genome duplication events in the Brassicales, and suggest that genes in mobile gene families are under different selection pressure than syntenic genes.
The objective of this project is to produce a large user-friendly and research-oriented database of the national measures that transpose EU directives into domestic law in order to provide the basis for large-N theoretically-based academic research on the causes of variance in the timing and quality of national implementation. The pre-existing databases on national transposition were not designed to facilitate academic research. The Commission publishes an annual report on the application of Community law, but it lists only the proportion of directives that have been transposed up to a given year, without providing data on delay of transposition. It also uses cumulative data since 1960, creating a severe upward bias in the national compliance record. The report tends to consider a directive transposed when the first measure has been notified by the Member States. But transposition normally needs more than one national measure, so the Commission’s data are likely overestimated compliance. Finally, no information is provided on the dates and instruments of national transposition. Recently, Celex, the EU legal database, has been opened to the public for free online consultation. The database contains approximately 3,000 directives, but it is not designed in a way that could be immediately used for research purposes. Fields than contains dates need to be converted from string to date format. Deadlines for the adoption of national measures need to be separated from deadlines for the application. There are no fields containing the dates of adoption of national transposition measures. These need to be deducted from the title of national implementing measures or by other available means. Moreover, there are no fields that record the types on national measures. Finally, there are mistakes and missing information that need correcting and completing. The project consists of migrating the raw data available from Celex into an appropriately-designed database.
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Summary from Dryad:
"Abstract:
Transposons are mobile genetic elements that are found in nearly all organisms, including humans. Mobilization of DNA transposons by transposase enzymes can cause genomic rearrangements, but our knowledge of human genes derived from transposases is limited. In this study, we find that the protein encoded by human PGBD5, the most evolutionarily conserved transposable element-derived gene in vertebrates, can induce stereotypical cut-and-paste DNA transposition in human cells. Genomic integration activity of PGBD5 requires distinct aspartic acid residues in its transposase domain, and specific DNA sequences containing inverted terminal repeats with similarity to piggyBac transposons. DNA transposition catalyzed by PGBD5 in human cells occurs genome-wide, with precise transposon excision and preference for insertion at TTAA sites. The apparent conservation of DNA transposition activity by PGBD5 suggests that genomic remodeling contributes to its biological function."
Open Government Licence - Canada 2.0https://open.canada.ca/en/open-government-licence-canada
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The FEDs described in this record were proclaimed by the Representation Orders of September 22, 2023. They will be in effect for a federal general election called any time after April 22, 2024. By-elections called after April 22, 2024, but before a general election would be conducted under the 2013 Representation Order. As part of the Canada Elections Act, the Chief Electoral Officer (CEO) must transpose the results of the previous general election if it had been conducted under the new boundaries. To fulfill our obligations under the Act, Elections Canada transposed the vote of the 44th general election, which was held on September 21, 2021. The transposition of the vote is a process where the votes per polling station, advanced polling station and special voting ballots are proportionally assigned to the FEDs under the new representation orders.
Somatic transposition in mammals and insects could increase cellular diversity and neural mobilization has been implicated in age-dependent decline. To understand the impact of transposition in somatic cells it is essential to reliably measure the frequency and map locations of new insertions. Here we identified thousands of putative somatic transposon insertions in neurons from individual Drosophila melanogaster using whole-genome sequencing. However, the number of de novo insertions did not correlate with transposon expression or fly age. Analysing our data with exons as "immobile genetic elements" revealed a similar frequency of unexpected exon translocations. A new sequencing strategy that recovers transposon : chromosome junction information revealed most putative de novo transposon and exon insertions likely result from unavoidable chimeric artefacts. Reanalysis of other published data suggests similar artefacts are often mistaken for genuine somatic transposition. We conclude tha...
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Additional file 3. Yeast excision frequency data sets.
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ABSTRACT This study aimed to identify the floristic composition and density of a seed bank transposed from a Seasonal Forest fragment in Rio Grande do Sul. The seed bank of the (BSI) fragment center and edge (BSII) of another forest fragment was also evaluated, both at medium to advanced successional stages. The seed bank was deposited in Brown-Gray Argisol and Red Argisol using exposed soil plots as control. ANOVA followed by Tukey test (p
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This dataset was created by JasonHuang
Released under CC0: Public Domain
Table from the American Community Survey (ACS) 5-year series on race and ethnicity related topics for City of Seattle Council Districts, Comprehensive Plan Growth Areas and Community Reporting Areas. Table includes B03002 Hispanic or Latino Origin by Race, B02008-B02013 Race Alone or in Combination with One or More Other. Data is pulled from block group tables for the most recent ACS vintage and summarized to the neighborhoods based on block group assignment.Table created for and used in the Neighborhood Profiles application.Vintages: 2023ACS Table(s): B03002, B02008, B02009, B02010, B02011, B02012, B02013Data downloaded from: Census Bureau's Explore Census Data The United States Census Bureau's American Community Survey (ACS):About the SurveyGeography & ACS</
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Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacterales are prevalent worldwide and pose an alarming threat to public health. The incidence and transmission of blaKPC-2 gene via horizontal gene transfer (e.g. transposition) have been well documented. However, the dynamics of transposon structure bearing blaKPC-2 and their exact effects on the evolution and dissemination of blaKPC-2 gene are not well characterized. Here, we collected all 161 carbapenem-resistant Enterobacterales (CRE) isolates during the early stage of CRE pandemic. We observed that the prevalence of KPC-2-producing Enterobacterales was mediated by multiple species and sequence types (STs), and that blaKPC-2 gene was located on three diverse variants of Tn1721 in multi-drug resistance (MDR) region of plasmid. Notably, the outbreak of KPC-2-producing plasmid is correlated with the dynamics of transposon structure. Furthermore, we experimentally demonstrated that replicative transposition of Tn1721 and IS26 promotes horizontal transfer of blaKPC-2 and the evolution of KPC-2-producing plasmid. The Tn1721 variants appearing concurrently with the peak of an epidemic (A2- and B-type) showed higher transposition frequencies and a certain superior ability to propagation. Overall, our work suggests replicative transposition contributes to the evolution and transmission of KPC-2-producing plasmid and highlights its important role in the inter- and intra-species dissemination of blaKPC-2 gene in Enterobacterales.
Data set from the article Lo Rito M, Raso I, Saracino A, Basile DP, Varrica A, Reali M, Carminati M, Frigiola A, Giamberti A. Primary Arterial Switch Operation for Late Presentation of Transposition of the Great Arteries With Intact Ventricular Septum. Semin Thorac Cardiovasc Surg. 2020 Nov 26:S1043-0679(20)30425-1. doi: 10.1053/j.semtcvs.2020.11.030. Epub ahead of print. PMID: 33249102.
This is the abstract:
Transposition of the great arteries (TGA) with intact ventricular septum (IVS) are subject to neonatal arterial switch operation (ASO) to avoid deconditioning of the left ventricle (LV). Often an early repair is not feasible in developing countries where late referral is frequent. We aim to explore ASO outcomes in late TGA-IVS and compare "unfavorable candidates" (LV myocardial mass <35gr/m2 unfavorable geometry. banana-like LV shape) with other late TGA-IVS. Single-center retrospective study on late TGA-IVS who underwent primary ASO between 2015 and 2018. We divide patients into 2 groups: unfavorable candidates and favorable candidates TGA. We report categorical variables as number and percentage and continuous variables as median with interquartile range. Differences were assessed with Chi-squared or Fisher exact tests, Wilcoxon sign-rank, and Wilcoxon-rank sum tests. 45 TGA-IVS were referred with a median age of 35 days. Pre-operative echocardiography identified banana-like LV shape in 66.7%; unfavorable LV geometry in 47.6% and LV mass <35gr/m2 in 51%. Only 1 death occurred related to myocardial ischemia. Five patients (11%) required ECMO for LV dysfunction, with unfavorable candidates having a higher but not significant use (18.7% vs 6.9%, P = 0.33). At discharge, echocardiography demonstrated significant LV mass improvement compared to pre-operative (58.6 vs 33.8 gr/m2, P < 0.0001) with no significant echocardiographic difference between unfavorable and favorable late-TGA. Primary ASO in late presenter TGA-IVS can be performed safely with low mortality having a low threshold to ECMO. Significantly unconditioned LV are amenable with primary ASO with good LV mass recovery.
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This dataset is about artists. It has 1 row and is filtered where the artworks is Transposition II Wall Hanging. It features 9 columns including birth date, death date, country, and gender.
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This dataset contains: - raw NMR data for new compounds used in the publication - comutational data for calculations presented in the published work - raw analytical data for catalysis run characterization - crysallographic data for new complexes
https://ega-archive.org/dacs/EGAC00001003295https://ega-archive.org/dacs/EGAC00001003295
This dataset contains CLCNKA/CLCNKB locus alignment data from 27 patients with Bartter syndrome and structural variants encompassing the CLCNKB gene. Due to data protection regulations and in accordance with the patient consent, only relevant alignments from the following regions are shared: hg19: chr1:16,300,000-16,400,000 hg38 (linked read dataset only): chr1:16,000,000-16,100,000
Methods to generate libaries were: long-range amplicon PCR (24 samples), targeted long-fragment enrichment (Samplix/Xdrop technology, 4 samples), long-read whole genome (PacBio Sequel II HiFi reads, 3 samples), 10X linked read short read whole genome (1 sample).
High-throughput techniques for detecting DNA polymorphisms generally do not identify changes in which the genomic position of a sequence, but not its copy number, varies among individuals. To explore such balanced structural polymorphisms, we used array-based Comparative Genomic Hybridization (aCGH) to conduct a genome-wide screen for single-copy genomic segments that occupy different genomic positions in the standard laboratory strain of Saccharomyces cerevisiae (S90) and a polymorphic wild isolate (Y101) through analysis of six tetrads from a cross of these two strains. Paired-end high-throughput sequencing of Y101 validated four of the predicted rearrangements. The transposed segments contained one to four annotated genes each, yet crosses between S90 and Y101 yielded mostly viable tetrads. The longest segment comprised 13.5 kb near the telomere of chromosome XV in the S288C reference strain and Southern blotting confirmed its predicted location on chromosome IX in Y101. Interestingly, inter-locus crossover events between copies of this segment occurred at a detectable rate. The presence of low-copy repetitive sequences at the junctions of this segment suggests that it may have arisen through ectopic recombination. Our methodology and findings provide a starting point for exploring the origins, phenotypic consequences, and evolutionary fate of this largely unexplored form of genomic polymorphism. Overall design: Array CGH
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This dataset is about books. It has 1 row and is filtered where the book is The compassionate God : an exercise in the theology of transposition. It features 6 columns including author, publication date, book publisher, and ISBN.
resultats-elections-transpose
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ABSTRACT Objective: The present article aims at reporting the clinical case of a patient who suffered trauma at two years of age, causing almost complete apical displacement of the deciduous maxillary left central incisor and of the permanent incisor. Methods: Ectopia secondary to intrusion was minimized by surgical removal of the ectopic tooth, and the left permanent canine was submitted to orthodontic traction to replace the extracted tooth. Results: The treatment period lasted 36 months, resulting in correct occlusion and a good aesthetic outcome. Conclusions: Dental transposition carried out by means of orthopedic traction is a good alternative in cases of a very unfavorable ectopic tooth position.
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