Abstract: Emperor penguins (Aptenodytes forsteri) and leopard seals (Hydrurga leptonyx) are iconic, top predators in Antarctica. Understanding their physiological ecology is essential to the assessment of their adaptability to the threats of climate change, pollution, and overfishing. The proposed research has multipronged objectives. Prior results suggest that Emperor penguins have flexible (vs. static) aerobic dive limits (ADL) that vary with the type of dive, and that the role of heart rate in utilization of oxygen stores also varies with dive type. A series of physiological measurements are proposed with backpack electrocardiogram recorders, that will allow further delineation of patterns and interrelationships among heart rate, dive behavior, and oxygen stores. Importantly, the research will be done on free diving emperors, and not individuals confined to a dive hole, thereby providing a more genuine measure of diving physiology and behavior. A separate objective is to examine foraging behavior of leopard seals, using a backpack digital camera and time depth recorder. Leopard seal behavior and prey intake is poorly quantified, but known to be significant. Accordingly the research is somewhat exploratory but will provide important baseline data. Finally, the P.I. proposes to continue long term overflight censuses of Emperor penguin colonies in the Ross Sea. Broader impacts include collaboration with National Geographic television, graduate student training, and development of sedation techniques for leopard seals.

The emperor penguin, Aptenodytes forsteri, is the premier avian diver and a top predator in the Antarctic ecosystem. The routine occurrence of 500-m diver during foraging trips to sea is both a physiological and behavior enigma. The objectives of this project address how and why emperors dive as deep and long as they do. The project examines four major topics in the diving biology of emperor penguins: pressure tolerance, oxygen store management, end-organ tolerance of diving hypoxemia/ischemia, and deep-dive foraging behavior. These subjects are relevant to the role of the emperor as a top predator in the Antarctic ecosystem, and to critical concepts in diving physiology, including decompression sickness, nitrogen narcosis, shallow water blackout, hypoxemic tolerance, and extension of aerobic dive time. The following hypotheses will be tested: 1) Prevention of nitrogen narcosis and decompression sickness in emperor penguins is achieved by inhibition of pulmonary gas exchange at depth. 2) Shallow water black out does not occur because of greater cerebral hypoxemic tolerance, and, in deep dives, because of resumption of pulmonary gas exchange during final ascent. 3) The rate of depletion of the blood oxygen store is a function of depth of dive and heart rate. 4) The aerobic dive limit (ADL) reflects the onset of lactate accumulation in locomotory muscle, not total depletion of all oxygen stores. 5) Elevation of tissue antioxidant capacity and free-radical scavenging enzyme activities protect against the routine ischemia/reperfusion which occur during diving. 6) During deep dives, the Antarctic silverfish, Pleuorogramma antarcticum, is the primary prey item for emperors. In addition to evaluation of the hypotheses below, the project has broader impacts in several areas such as partnership with foreign and national institutes and organizations (e.g., the National Institute of Polar Research of Japan, Centro de Investigacioines del Noroeste of Mexico, National Geographic, the University of Texas Southwestern Medical Center, and Sea World). Participation in National Geographic television documentaries will provide unique educational opportunities for the general public; development of state-of-the-art technology (e.g., blood oxygen electrode recorders, blood samplers, and miniaturized digital cameras) will lay the groundwork for future research by this group and others; and the effects of the B15 iceberg on breeding success of emperor penguins will continue to be evaluated with population censuses during planned fieldwork at several Ross Sea emperor penguin colonies.

Metadata record for data from ASAC Project 484 See the link below for public details on this project. ---- Public Summary from Project ---- Emperor penguins are the only birds that breed in the Antarctic winter. They feed mainly on fish and squid but also ingest krill. Changes in food availability due to oceanographic or climatic factors, or to the extent of sea ice (through the processes of global warming) will have a direct impact on the breeding success and population size of the penguins. By counting the number of males that incubate at mid-winter each year, we can monitor trends in their population size. Counts of fledglings in spring (November) tell us how successful the penguins bred. The download file contains an excel spreadsheet which presents a summary of known Emperor Penguin colonies in the area of the Australian Antarctic Territory (AAT), and a file which details counts of male emperor penguins at the Taylor Glacier colony. A description of the column headings used in the spreadsheet is below. Colony: Colony name lat, long: latitude and longitude of colony discovered: date colony was discovered current est pop (BP): Current estimated population size in breeding pairs - current as at date the colony was last seen last seen: date the colony was last seen counting method: method used to count the breeding pairs in the colony comments: any applicable comments reference: references relating to the colony Taken from the 2009-2010 Progress Report: Public summary of the season progress: Population size of colonies fluctuates which is why long term monitoring studies are necessary to detect trends. At the emperor penguin colony at Taylor Glacier, monitored continuously since 1988, a slight downward trend is apparent but is not (yet?) statistically significant. The colony was visited three times: once in winter to obtain an estimate of the number of adults in the colony (roughly equivalent to the number of breeding pairs), and twice during the late chick rearing season to estimate breeding success. The count of adults in 2009 was the lowest on record. Reasons for this are still unknown.

Metadata record for data from ASAC Project 1252 See the link below for public details on this project.

Currently three datasets are attached to this metadata record. Dive data collected in 1988, track data from adult birds collected in 1994 and track data from fledglings collected in 1995.

Dive data are available in Microsoft Word format, while the track data are available in Microsoft Excel format.

A readme file (txt) is included in each download file to explain column headings, etc.

---- Public Summary from Project ----
To breed successfully the winter-breeding emperor penguins must fatten on two occasions: once before the onset of moult in January, and again prior to the commencement of the new breeding season in March. Interference with the capacity of the penguins to fatten in summer might be detrimental to the their breeding performance and survival later on in winter. This study seeks to determine the likely impact of commercial fishing operations on emperor penguin colonies at the Mawson Coast. More specifically, the data pertains to the locations of emperor penguins when fattening prior to the moult, and prior to the new breeding season.

Project objectives:
1. To determine the extent and location of foraging areas of post-breeding adult Emperor penguins in summer.
3. To determine the extent and locations of foraging areas of fledgling Emperor penguins on their first trip to sea.
4. To identify interseasonal and interannual variations in foraging areas in conjunction with changes in seaice conditions and compare these with results from different colonies.
5. To survey the coastline of the AAT to verify the existence (or non-existence) of Emperor penguin colonies.

Emperor penguins are icons of Antarctic wildlife and their conservation is of paramount interest to the wider community. They are also key consumers of marine resources in several areas and consequently there is great potential for interactions between feeding penguins and harvesting of fish and krill. Emperor penguins are one of the few species to breed on the fast ice (although there are three known land-based colonies, one of which has all but ceased to exist in recent years). Thus, the breeding habitat of Emperor penguins is subject to direct alteration as a result of climate change. Colonies of Emperors are found across a wide latitudinal range, from deep in the Ross Sea to the tip of the Antarctic Peninsula. This range includes breeding areas where significant changes in seaice are not (yet?) thought to be occurring to areas where seaice is changing rapidly. Accordingly, studies at multiple locations will provide valuable clues on how this species will be affected by a warming Antarctic. Additionally, Emperor penguins are large animals that live in a relatively small number of discrete locations. It is therefore more than feasible, using an international effort, to study an entire species and to make some predictions about their response to a warming world and to current and future fishing practices. This project aims to make the first steps towards an overall conservation assessment of Emperor penguins through studies in several locations around the Antarctic continent. Should these attempts be successful, then a more ambitious international project will be launched to take a species-wide perspective.

The emperor penguin dives deeper and longer, fasts longer, and endures the harshest weather conditions of all diving birds. It spends about four and half months per annum deep in Antarctic pack ice away from shore and stations, and thus is largely unavailable for study. This time includes preparation for the molt, and travel to the colony to breed, a time period in which great swings in body weight occur. This study will fill an important gap in what we know about the biology of the annual cycle of the emperor by examining the molt-post molt period. The P.I. proposes to traverse the Amundsen and Bellingshausen seas on the Oden, to locate and tag emperor penguins during the molt season. The objectives are to (1) Place satellite tags on 20 adult post molt birds to determine their route, rate of travel, and diving behavior as they return back to their breeding colonies, (2) Obtain an index of body condition, (3) Collect guano to determine the type of food consumed by emperor penguins in the region, (4) Conduct shipboard surveys to sight and plot the location and abundance of adult and juvenile birds on the ship's track. The PI hypothesizes that bird dives will be shallow during the initial post-molt phase, and that food will consist primarily of krill; that there will be differential dispersal of birds from the Ross Sea vs. Marie Byrd Land, with Ross Sea birds traveling farther; and that the greatest adult mortality occurs during the molt and early post molt period. Broader impacts include training of a post doc, a graduate student, and an aquarium volunteer. The P.I. also will present findings through a website, through public lectures, and in collaboration with the Birch aquarium.

Metadata record for data from ASAC Project 2954.
See the link below for public details on this project.

Public
The primary goal of this project is to determine the status and origin of diseases in Emperor Penguins at Auster Rookery near Mawson Station, Antarctica. We will investigate the origins of such disease and the role humans may have played. We will sample adults and chicks in order to isolate and describe the pathogens. A high percentage of Emperor Penguin chicks have antibodies to infectious bursal disease (IBDV). This study will investigate the role the adults play in transmitting IBDV to their chicks. The high prevalence of IBDV antibodies should help us to isolate the virus and discover its origin.

Project objectives:
Status of Disease in the Emperor Penguins of Auster Rookery

1) To determine the prevalence of disease in adult Emperor Penguins (Aptenodytes forsteri) at Auster Rookery. Over 65% of Emperor Penguin chicks at Auster Rookery had serum antibodies to Infectious Bursal Disease Virus (IBDV) in December 1995 (Gardner et al. 1997). We have no information on the presence of the same antibodies on adult Emperor Penguins. We will focus on IBDV, but will also test for other common avian diseases.

2) To repeat the sampling of Emperor Penguin Chicks by Gardner et al. (1997) in order to compare the prevalence of IBDV in Emperor Penguin chicks in 2008 with 1995.

3) To determine the seasonal progression of IBDV antibody prevalence in both adults and chicks.

4) To determine the possible source(s) of viral infection in Emperor Penguin chicks. Because Gardner et al. (1997) had no information on adult Emperor Penguins we do not know how the chicks are exposed to IBDV. Gardner et al. (1997) suggested that poultry waste from the nearby Mawson Station may be a source of virus for the Emperor Penguin chicks. Penguins do not scavenge food, however, so the source of infection must be either from the environment, local predators/scavenger, or from parents feeding their young. By sampling both adults and chicks in different parts of the season, we will determine when antibodies first appear in the chicks. If they have antibodies in the early season, then scavengers/predators can not account for their exposure to IBDV.

5) To determine the source of the IBDV. We will attempt to isolate virus from Emperor Penguins in order to identify the strains responsible for the antibody reactions in Emperor Penguins. Using reverse transcription polymerase chain reaction (RT-PCR), we will sequence genes from the virus which can be compared with known gene sequences from serotypes available from GenBank.

6) To monitor the chick mortality and conduct field necropsies of the Emperor Penguins at the Auster colony to determine whether IBDV or other diseases are a factor in reproductive success.

7) To contribute to a conservation strategy for Antarctic wildlife. By developing information on the importance and origins of disease in Emperor Penguins we can clarify the role of human visitors in the transmission of disease in Antarctic wildlife.

Progress against objectives:
Excellent progress has been made towards all the stated goals.

By spending the winter of 2008 at Mawson Station we had access to the emperor penguin colony at Auster. We successfully sampled 400 adults and 200 chicks as stated in the proposal. We now have the samples back in Australia and analyses are beginning. No sample analysis for disease could be undertaken while still in Antarctica.

The samples will 1) give us a determination of the prevalence of IBDV antibodies (also some other disease viruses) for both adults and chicks. 2) One of our sampling periods was a repeat of the sampling conducted by Gardner et al. so we can compare the prevalence of IBDV antibodies in chicks from 2 different years and relate that to the prevalence in adults. 4) We conducted our sampling at four different times during the winter so that we were able to sample chicks before any other species visited the colony, then sample them again 6 weeks after skuas and giant petrels were in the area. 5) In order to determine the source of IBDV we want to determine its RNA sequence. To that end, a full set of samples have been sent to Dr. Daral Jackwood at Ohio State University, a colleague and IBDV expert. He has just begun to analyse the samples to isolate and sequence the IBDV RNA. 6) We monitored chick mortality with visits to the colony on average once per week. We noted approximately 800 dead chicks and collected 120 of the chicks for field necropsies. They mostly died of starvation with a few exceptions. We found parasites in one dead chick. We also collected 9 carcasses of adult emperor penguins. Eight of the 9 were females who all died with complications of egg laying. 7) This goal will require the completion of all the analyses for us to make conservation recommendations.



This collection of files represents the data (including samples) collected for Project 2954 on Emperor Penguins.

This project was based on collecting samples from Emperor penguins throughout the winter season. There were 5 sets of samples.
1-Adults during courtship in May
2-Adults during hatch period in early August
3-Chicks at ca 5 weeks of age--soon after leaving the constant care of their parents
4- Adults in early summer (mid-November)
5-Chicks in early summer (late November)

Sampling at these intervals it was hoped we would be able to discriminate when or how the IBD antibodies appear in this population.


The files are:
1) Project 2954-Emperor IBD Antibodies PrevalenceSummary
This is a summary file of all the Virus Neutralization tests to determine the presence of antibodies to Infectious Bursal Disease (IBD) in the 5 groups of Emperor Penguins.

2) Project 2954-Emperor IBD summary graph
This is a graphical representation of the summary information for IBDV antibodies

3) Project 2954-Emperor Penguin Egg Samples-2008
This is a listing of the abandoned eggs collected over the winter. The eggs were weighed and measured. Many were sampled. We took 5ml of yolk and stored the samples in -80 C for future analysis.

4) Project 2954-Emperor Penguin sampling2008
This is the master file of sampling. Each penguin we handled was given a sample identification (but no permanent identifying marks). Each was weighed, measured, inspected for ticks and samples taken. This file identifies which samples were obtained. In addition it represents some analyses. The sexes of many of the penguins have been determined genetically with PCR. That is included in the file.

5) Project 2954-Emperor Penguin Serology NDV and AI 2008
This file is a listing of all the serum samples and the results of Hemagglutination Inhibition test (HAI) for antibodies to Newcastle Disease Virus (NDV) and an antibody ELISA test for Avian Influenza. No sign of any exposure to NDV or AI in Emperor penguins.

6) Project 2954-Disease Status Poster-Peng Conf 2010
This Powerpoint file is a single oversized page that presents much of the results that we have to date. It was presented at the 7th International Conference on the Biology of Penguins, in Boston USA, Sept 2010

7) Project 2954-Searching for IBDV in Emperors with RT-PCR
This MS WORD file is the summary of methods and results of testing tissue samples from chick carcasses found at the colony. Bursa and spleen tissue was preserved and sent to Daral Jackwood in Ohio USA. He conducted real time Reverse Transcriptase PCR on the samples to try to find the IBD virus. That is essential for the full identification of the source of antibodies in these penguins. Unfortunately all results were all negative. We hope with a new proposal to expand our testing to find this virus.

This dataset includes an inventory of emperor penguins captured after their molt in February 2023. Observations recorded include capture date, instrumentation, body mass, flipper length, and samples collected.

Emperor penguin neutral SNP datasetEP_final.vcf

Comparison between at-sea-ecological studies that equipped emperor penguins over the last 30 years.

High-resolution wildlife photography of emperor penguins in their natural Antarctic habitat, featuring full-body detection annotations for penguin individuals.

Creching emperor penguin (Aptenodytes forsteri) chickswere exposed to two overflights by an S-76 twin engine helicopter at 1000 m: a current operational guideline for helicopter activity in Antarctica. The flights were conducted on the same day but under different wind conditions: a morning flight with a 10 kt (18 km.hr-1) katabatic blowing perpendicular to the direction of helicopter travel and an afternoon flight with virtually no wind. Background noise levels recorded in the morning, before the helicopter flight, were significantly higher than in the afternoon, but these differences were not detectable when the helicopter was overhead. There were also no significant differences in the way chicks responded to helicopters between the morning and afternoon flight. All chicks became more vigilant when the helicopter approached and 69% either walked or ran, generally moving less than 10 m toward other chicks (i.e. not scattering). Most chicks (83%) displayed flipper-flapping, probably indicating nervous apprehension. This behaviour was seldom displayed in the absence of disturbance. Although all effects were relatively transitory, results support the introduction of more conservative guidelines for helicopter operations around breeding localities of this species.

The fields in this dataset are:

Time Action Date Lying Standing Walking Preening Flapping

Antarctic wildlife images featuring emperor penguins in their natural icy habitat, annotated for object detection to identify and track these flightless birds.

Locations for all known colonies are taken from published analyses [8]. Sea ice trend values are also taken from recent analyses [13], with values for each colony location represented by the geographically closest pixels. Shaded values indicate colonies that are in areas of current significant sea ice loss.

Adult male and female emperor penguins (Aptenodytes forsteri) were fitted with satellite transmitters at Pointe-Géologie (Adélie Land), Dumont d'Urville Sea coast, in November 2005. Nine of 30 data sets were selected for analyses to investigate the penguins' diving behaviour at high resolution (doi:10.1594/PANGAEA.633708, doi:10.1594/PANGAEA.633709, doi:10.1594/PANGAEA.633710, doi:10.1594/PANGAEA.633711). The profiles are in synchrony with foraging trips of the birds during austral spring (doi:10.1594/PANGAEA.472171, doi:10.1594/PANGAEA.472173, doi:10.1594/PANGAEA.472164, doi:10.1594/PANGAEA.472160, doi:10.1594/PANGAEA.472161). Corresponding high resolution winter data (n = 5; archived elsewhere) were provided by A. Ancel, Centre d'Ecologie et Physiologie Energétiques, CNRS, Strasbourg, France. Air-breathing divers tend to increase their overall dive duration with increasing dive depth. In most penguin species, this occurs due to increasing transit (descent and ascent) durations but also because the duration of the bottom phase of the dive increases with increasing depth. We interpreted the efficiency with which emperor penguins can exploit different diving depths by analysing dive depth profile data of nine birds studied during the early and late chick-rearing period in Adélie Land, Antarctica. Another eight datasets of dive depth and duration frequency recordings (doi:10.1594/PANGAEA.472150, doi:10.1594/PANGAEA.472152, doi:10.1594/PANGAEA.472154, doi:10.1594/PANGAEA.472155, doi:10.1594/PANGAEA.472142, doi:10.1594/PANGAEA.472144, doi:10.1594/PANGAEA.472146, doi:10.1594/PANGAEA.472147), which backup the analysed high resolution depth profile data, and dive depth and duration frequency recordings of another bird (doi:10.1594/PANGAEA.472156, doi:10.1594/PANGAEA.472148) did not match the requirement of high resolution for analyses. Eleven additional data sets provide information on the overall foraging distribution of emperor penguins during the period analysed (doi:10.1594/PANGAEA.472157, doi:10.1594/PANGAEA.472158, doi:10.1594/PANGAEA.472162, doi:10.1594/PANGAEA.472163, doi:10.1594/PANGAEA.472166, doi:10.1594/PANGAEA.472167, doi:10.1594/PANGAEA.472168, doi:10.1594/PANGAEA.472170, doi:10.1594/PANGAEA.472172, doi:10.1594/PANGAEA.472174, doi:10.1594/PANGAEA.472175).

1. Long-term photographic recordings of animal populations provide unique insights in ecological and evolutionary processes. However, image acquisition at remote locations under harsh climatic conditions is highly challenging. 2. We present a robust, energetically self-sufficient and remote-controlled observatory designed to operate year-round in the Antarctic at temperatures below -50 °C and wind speeds above 150 km/h. The observatory is equipped with multiple overview cameras and a high resolution steerable camera with a telephoto lens for capturing images with high spatial and temporal resolution. 3. Our observatory has been in operation since 2013 to investigate an emperor penguin (Aptenodytes forsteri) colony at Atka Bay near the German Neumayer III research station. Data recorded by this observatory give novel biological insights in animal life cycle and demographic trends, but also in collective and individual behaviour. As an example, we present data showing how wind speed and direction influence movements of the entire colony and of individual penguins. We also estimate daily fluctuations in the total number of individuals present at the breeding site. 4. Our results demonstrate that remote-controlled observation systems can bridge the gap between remote sensing, simple time-lapse recording setups, and on-site observations by human investigators to collect unique biological datasets of undisturbed animal populations.

MEE_SPOT_Fig5_ColonyLocomotion Meteorological data and time lapse image recordings (04/02/2013 to 04/07/2013) used to evaluate the influence of wind speed and wind direction on the position of the Atka Bay emperor penguin colony MEE_SPOT_Fig6_SingleLocomotion Video (4008x2672, 5 fps, 60s) recorded on 07/22/2013 at 11:40:47 UTC used to evaluate the movement characteristics of single emperor penguins at the huddle boundaries MEE_SPOT_Fig4_Abundance High resolution panoramic images for 04/01/2014 to 04/21/2014 used to evaluate emperor penguin numbers and arrival pattern

The derivation of a model ensemble useful for predicting changes in penguin habitat.

As seabirds emperor penguins spent a large proportion of their lives at sea. For food they depend entirely on marine resources. Young penguins rarely return to their natal colonies after their first year. Satellite tracking will give us insights into where foraging areas may be that are important for these birds. This tracking work is part of a multi-species study funded by the Integrated Marine Observation System (IMOS).

These data are from penguins from the Amanda Bay area, and for the 2010-2011 season.

These files do not have any missing values. The data sets are ready for export or copy into any analysis program.

• Consensus SNP calls dataset from RADseq data for the Emperor penguin.Input files for the analyses described in Cristofari et al. 2016, "Full circumpolar migration ensures evolutionary unity in the Emperor penguin":- Extended Bayesian Skyline Plot input files,- fastsimcoal2 analysis input files,- ngsAdmix genotype likelihood input file,- generic Structure / fastStructure input file,- Migrate-n analysis input files,

This dataset contains physiological samples, including blood, feather, scat, and whisker material, collected from Emperor Penguins (Aptenodytes forsteri)and Weddell Seals (Leptonychotes weddellii) at Cape Crozier and across the Ross Sea in 2024. The samples were collected to investigate diet composition and population genetic structure using DNA analysis. These data support research into trophic ecology, foraging behaviour, and connectivity of Antarctic marine predator populations within the Ross Sea ecosystem.

GET DATA: michelle.larue@canterbury.ac.nz