We describe a bibliometric network characterizing co-authorship collaborations in the entire Italian academic community. The network, consisting of 38,220 nodes and 507,050 edges, is built upon two distinct data sources: faculty information provided by the Italian Ministry of University and Research and publications available in Semantic Scholar. Both nodes and edges are associated with a large variety of semantic data, including gender, bibliometric indexes, authors' and publications' research fields, and temporal information. While linking data between the two original sources posed many challenges, the network has been carefully validated to assess its reliability and to understand its graph-theoretic characteristics. By resembling several features of social networks, our dataset can be profitably leveraged in experimental studies in the wide social network analytics domain as well as in more specific bibliometric contexts. , The proposed network is built starting from two distinct data sources:

the entire dataset dump from Semantic Scholar (with particular emphasis on the authors and papers datasets) the entire list of Italian faculty members as maintained by Cineca (under appointment by the Italian Ministry of University and Research).

By means of a custom name-identity recognition algorithm (details are available in the accompanying paper published in Scientific Data), the names of the authors in the Semantic Scholar dataset have been mapped against the names contained in the Cineca dataset and authors with no match (e.g., because of not being part of an Italian university) have been discarded. The remaining authors will compose the nodes of the network, which have been enriched with node-related (i.e., author-related) attributes. In order to build the network edges, we leveraged the papers dataset from Semantic Scholar: specifically, any two authors are said to be connected if there is at least one pap..., , # Data cleaning and enrichment through data integration: networking the Italian academia

https://doi.org/10.5061/dryad.wpzgmsbwj

Description of the data and file structure

This repository contains two main data files:

• edge_data_AGG.csv, the full network in comma-separated edge list format (this file contains mainly temporal co-authorship information);
• Coauthorship_Network_AGG.graphml, the full network in GraphML format.Â

along with several supplementary data, listed below, useful only to build the network (i.e., for reproducibility only):

• University-City-match.xlsx, an Excel file that maps the name of a university against the city where its respective headquarter is located;
• Areas-SS-CINECA-match.xlsx, an Excel file that maps the research areas in Cineca against the research areas in Semantic Scholar.

Description of the main data files

The Coauthorship_Network_AGG.graphml is intended to be the core file which c...

Establishment of a syndication flow from the department of Vendée (85) with data from the “Festivals and Events” schedule with enriched data: name, address, type of FMA (Fests and events), means of communication (landline phone, e-mail and website), social networks, GPS coordinates, labels, languages spoken, methods of payment accepted, opening dates, rates, details of visits, videos.

Our dataset provides detailed and precise insights into the business, commercial, and industrial aspects of any given area in the USA (Including Point of Interest (POI) Data and Foot Traffic. The dataset is divided into 150x150 sqm areas (geohash 7) and has over 50 variables. - Use it for different applications: Our combined dataset, which includes POI and foot traffic data, can be employed for various purposes. Different data teams use it to guide retailers and FMCG brands in site selection, fuel marketing intelligence, analyze trade areas, and assess company risk. Our dataset has also proven to be useful for real estate investment.- Get reliable data: Our datasets have been processed, enriched, and tested so your data team can use them more quickly and accurately.- Ideal for trainning ML models. The high quality of our geographic information layers results from more than seven years of work dedicated to the deep understanding and modeling of geospatial Big Data. Among the features that distinguished this dataset is the use of anonymized and user-compliant mobile device GPS location, enriched with other alternative and public data.- Easy to use: Our dataset is user-friendly and can be easily integrated to your current models. Also, we can deliver your data in different formats, like .csv, according to your analysis requirements. - Get personalized guidance: In addition to providing reliable datasets, we advise your analysts on their correct implementation.Our data scientists can guide your internal team on the optimal algorithms and models to get the most out of the information we provide (without compromising the security of your internal data).Answer questions like: - What places does my target user visit in a particular area? Which are the best areas to place a new POS?- What is the average yearly income of users in a particular area?- What is the influx of visits that my competition receives?- What is the volume of traffic surrounding my current POS?This dataset is useful for getting insights from industries like:- Retail & FMCG- Banking, Finance, and Investment- Car Dealerships- Real Estate- Convenience Stores- Pharma and medical laboratories- Restaurant chains and franchises- Clothing chains and franchisesOur dataset includes more than 50 variables, such as:- Number of pedestrians seen in the area.- Number of vehicles seen in the area.- Average speed of movement of the vehicles seen in the area.- Point of Interest (POIs) (in number and type) seen in the area (supermarkets, pharmacies, recreational locations, restaurants, offices, hotels, parking lots, wholesalers, financial services, pet services, shopping malls, among others). - Average yearly income range (anonymized and aggregated) of the devices seen in the area.Notes to better understand this dataset:- POI confidence means the average confidence of POIs in the area. In this case, POIs are any kind of location, such as a restaurant, a hotel, or a library. - Category confidences, for example"food_drinks_tobacco_retail_confidence" indicates how confident we are in the existence of food/drink/tobacco retail locations in the area. - We added predictions for The Home Depot and Lowe's Home Improvement stores in the dataset sample. These predictions were the result of a machine-learning model that was trained with the data. Knowing where the current stores are, we can find the most similar areas for new stores to open.How efficient is a Geohash?Geohash is a faster, cost-effective geofencing option that reduces input data load and provides actionable information. Its benefits include faster querying, reduced cost, minimal configuration, and ease of use.Geohash ranges from 1 to 12 characters. The dataset can be split into variable-size geohashes, with the default being geohash7 (150m x 150m).

Success.ai’s Startup Data with Contact Data for Startup Founders Worldwide provides businesses with unparalleled access to key entrepreneurs and decision-makers shaping the global startup landscape. With data sourced from over 170 million verified professional profiles, this dataset offers essential contact details, including work emails and direct phone numbers, for founders in various industries and regions.

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Establishment of a syndication flow from the department of Vendée (85) with the data from the “Restoration” schedule with enriched data: name, address, type of restaurant, means of communication (landline phone, e-mail and website), social networks, GPS coordinates, labels, languages spoken, payment methods accepted, opening dates, rates, details of visits, videos.

Charleston subdivision polygons with fields for: Average Year Built, Street Length, Sidewalk Length, Percent Sidewalks, Street Area, Street Tree Area, Percent Street Tree, Subdivision Tree Area, Percent Subdivision Tree, Paved Area, Percent Paved, Impervious Area, Percent Impervious, Morning Mean Temperature, Afternoon Mean Temperature, Evening Mean Temperature, Has Demographic Data, Total Population, Total Households, Black Population, Percent Black, Median Household Income, Population Below Poverty Line, Percent of Population Below Poverty Line, Population that Walks to Work, Percent of Population that Walks to Work, Households with 0 Vehicles, Percent of Households with 0 Vehicles, and Square Mileage. Areas and Percent calculated using ‘Summarize Within’ tool, demographic data obtained through ESRI’s ‘Enrich Data’ tool from American Community Survey data.

1. Targeted enrichment of conserved genomic regions (e.g., ultraconserved elements or UCEs) has emerged as a promising tool for inferring evolutionary history in many organismal groups. Because the UCE approach is still relatively new, much remains to be learned about how best to identify UCE loci and design baits to enrich them.

2. We test an updated UCE identification and bait design workflow for the insect order Hymenoptera, with a particular focus on ants. The new strategy augments a previous bait design for Hymenoptera by (a) changing the parameters by which conserved genomic regions are identified and retained, and (b) increasing the number of genomes used for locus identification and bait design. We perform in vitro validation of the approach in ants by synthesizing an ant-specific bait set that targets UCE loci and a set of “legacy” phylogenetic markers. Using this bait set, we generate new data for 84 taxa (16/17 ant subfamilies) and extract loci from an additional 17 genome-enabled taxa. We then use these data to examine UCE capture success and phylogenetic performance across ants. We also test the workability of extracting legacy markers from enriched samples and combining the data with published data sets.

3. The updated bait design (hym-v2) contained a total of 2,590-targeted UCE loci for Hymenoptera, significantly increasing the number of loci relative to the original bait set (hym-v1; 1,510 loci). Across 38 genome-enabled Hymenoptera and 84 enriched samples, experiments demonstrated a high and unbiased capture success rate, with the mean locus enrichment rate being 2,214 loci per sample. Phylogenomic analyses of ants produced a robust tree that included strong support for previously uncertain relationships. Complementing the UCE results, we successfully enriched legacy markers, combined the data with published Sanger data sets, and generated a comprehensive ant phylogeny containing 1,060 terminals.

4. Overall, the new UCE bait design strategy resulted in an enhanced bait set for genome-scale phylogenetics in ants and likely all of Hymenoptera. Our in vitro tests demonstrate the utility of the updated design workflow, providing evidence that this approach could be applied to any organismal group with available genomic information.

This feature service depicts the National Weather Service (NWS) watches, warnings, and advisories within the United States. Watches and warnings are classified into well over 100 categories. See event descriptions for full details. A warning is issued when a hazardous weather or hydrologic event is occurring, imminent or likely. A warning means weather conditions pose a threat to life or property. People in the path of the storm need to take protective action.A watch is used when the risk of a hazardous weather or hydrologic event has increased significantly, but its occurrence, location or timing is still uncertain. It is intended to provide enough lead time so those who need to set their plans in motion can do so. A watch means that hazardous weather is possible. People should have a plan of action in case a storm threatens, and they should listen for later information and possible warnings especially when planning travel or outdoor activities.An advisory is issued when a hazardous weather or hydrologic event is occurring, imminent or likely. Advisories are for less serious conditions than warnings, that cause significant inconvenience and if caution is not exercised, could lead to situations that may threaten life or property.SourceNational Weather Service RSS-CAP Warnings and Advisories: Public AlertsNational Weather Service Boundary Overlays: AWIPS Shapefile DatabaseSample DataSee Sample Layer Item for sample data during Weather inactivity!Update FrequencyThe services is updated every 5 minutes using the Aggregated Live Feeds methodology.The overlay data is checked and updated daily from the official AWIPS Shapefile Database.Area CoveredUnited States and TerritoriesWhat can you do with this layer?Customize the display of each attribute by using the Change Style option for any layer.Query the layer to display only specific types of weather watches and warnings.Add to a map with other weather data layers to provide insight on hazardous weather events.Use ArcGIS Online analysis tools, such as Enrich Data, to determine the potential impact of weather events on populations.Revisions:Feb 25, 2021: Revised service data upate workflow, improving stability and update interval.Process now checks for data updates every 5 minutes!Mar 3, 2021: Revised data processing to leverage VTEC parameter details to better align Event 'effective' dates with reported dates on Alert pages.Apr 17, 2023: Turned off popups for boundary Layers by default.Feb 1, 2024: Revised to leverage CAP v1.2 source endpoint. Update event link to use alert search.Feb 16, 2024: Revised event link to accomodate change in alert search endpoint.Jan 19, 2025: Added event 'Description' and 'Instructions', updated Pop-up.Jan 22, 2025: Exposed 'Hours Old' fields supporting last 'Updated', 'Effective', and 'Expiration' as +- age values for events.This map is provided for informational purposes and is not monitored 24/7 for accuracy and currency.If you would like to be alerted to potential issues or simply see when this Service will update next, please visit our Live Feed Status Page!

This feature service depicts the National Weather Service (NWS) watches, warnings, and advisories within the United States. Watches and warnings are classified into well over 100 categories. See event descriptions for full details. A warning is issued when a hazardous weather or hydrologic event is occurring, imminent or likely. A warning means weather conditions pose a threat to life or property. People in the path of the storm need to take protective action.A watch is used when the risk of a hazardous weather or hydrologic event has increased significantly, but its occurrence, location or timing is still uncertain. It is intended to provide enough lead time so those who need to set their plans in motion can do so. A watch means that hazardous weather is possible. People should have a plan of action in case a storm threatens, and they should listen for later information and possible warnings especially when planning travel or outdoor activities.An advisory is issued when a hazardous weather or hydrologic event is occurring, imminent or likely. Advisories are for less serious conditions than warnings, that cause significant inconvenience and if caution is not exercised, could lead to situations that may threaten life or property.SourceNational Weather Service RSS-CAP Warnings and Advisories: Public AlertsNational Weather Service Boundary Overlays: AWIPS Shapefile DatabaseSample DataSee Sample Layer Item for sample data during Weather inactivity!Update FrequencyThe services is updated every 5 minutes using the Aggregated Live Feeds methodology.The overlay data is checked and updated daily from the official AWIPS Shapefile Database.Area CoveredUnited States and TerritoriesWhat can you do with this layer?Customize the display of each attribute by using the Change Style option for any layer.Query the layer to display only specific types of weather watches and warnings.Add to a map with other weather data layers to provide insight on hazardous weather events.Use ArcGIS Online analysis tools, such as Enrich Data, to determine the potential impact of weather events on populations.Revisions:Feb 25, 2021: Revised service data upate workflow, improving stability and update interval.Process now checks for data updates every 5 minutes!Mar 3, 2021: Revised data processing to leverage VTEC parameter details to better align Event 'effective' dates with reported dates on Alert pages.Apr 17, 2023: Turned off popups for boundary Layers by default.Feb 1, 2024: Revised to leverage CAP v1.2 source endpoint. Update event link to use alert search.Feb 16, 2024: Revised event link to accomodate change in alert search endpoint.Jan 19, 2025: Added event 'Description' and 'Instructions', updated Pop-up.Jan 22, 2025: Exposed 'Hours Old' fields supporting last 'Updated', 'Effective', and 'Expiration' as +- age values for events.This map is provided for informational purposes and is not monitored 24/7 for accuracy and currency.If you would like to be alerted to potential issues or simply see when this Service will update next, please visit our Live Feed Status Page!

List of 1655 genes with a negative jGRP statistic meaning a down-regulation in LUAD tissues relative to normal tissues on the three LUAD data sets. Table S2. List of 1626 genes with a positive jGRP statistic meaning a up-regulation in LUAD tissues relative to normal tissues on the three LUAD data sets. Table S3. List of 42 KEGG pathways significantly enriched in the DEG lists of jGRP (τ = 0.7) by DAVID. Table S4. List of 57 KEGG pathways significantly enriched in the DEG lists of Fisher’s by DAVID. Table S5. List of 53 KEGG pathways significantly enriched in the DEG lists of AW by DAVID. Table S6. List of 40 KEGG pathways significantly enriched in the DEG lists of RP by DAVID. Table S7. List of 20 KEGG pathways significantly enriched in the DEG lists of Pooled cor by DAVID. (RAR 259 kb)

BackgroundHigh throughput molecular-interaction studies using immunoprecipitations (IP) or affinity purifications are powerful and widely used in biology research. One of many important applications of this method is to identify the set of RNAs that interact with a particular RNA-binding protein (RBP). Here, the unique statistical challenge presented is to delineate a specific set of RNAs that are enriched in one sample relative to another, typically a specific IP compared to a non-specific control to model background. The choice of normalization procedure critically impacts the number of RNAs that will be identified as interacting with an RBP at a given significance threshold – yet existing normalization methods make assumptions that are often fundamentally inaccurate when applied to IP enrichment data.MethodsIn this paper, we present a new normalization methodology that is specifically designed for identifying enriched RNA or DNA sequences in an IP. The normalization (called adaptive or AD normalization) uses a basic model of the IP experiment and is not a variant of mean, quantile, or other methodology previously proposed. The approach is evaluated statistically and tested with simulated and empirical data.Results and ConclusionsThe adaptive (AD) normalization method results in a greatly increased range in the number of enriched RNAs identified, fewer false positives, and overall better concordance with independent biological evidence, for the RBPs we analyzed, compared to median normalization. The approach is also applicable to the study of pairwise RNA, DNA and protein interactions such as the analysis of transcription factors via chromatin immunoprecipitation (ChIP) or any other experiments where samples from two conditions, one of which contains an enriched subset of the other, are studied.

This feature service depicts the National Weather Service (NWS) watches, warnings, and advisories within the United States. Watches and warnings are classified into 43 categories.A warning is issued when a hazardous weather or hydrologic event is occurring, imminent or likely. A warning means weather conditions pose a threat to life or property. People in the path of the storm need to take protective action.A watch is used when the risk of a hazardous weather or hydrologic event has increased significantly, but its occurrence, location or timing is still uncertain. It is intended to provide enough lead time so those who need to set their plans in motion can do so. A watch means that hazardous weather is possible. People should have a plan of action in case a storm threatens, and they should listen for later information and possible warnings especially when planning travel or outdoor activities.An advisory is issued when a hazardous weather or hydrologic event is occurring, imminent or likely. Advisories are for less serious conditions than warnings, that cause significant inconvenience and if caution is not exercised, could lead to situations that may threaten life or property.SourceNational Weather Service RSS-CAP Warnings and Advisories: Public AlertsNational Weather Service Boundary Overlays: AWIPS Shapefile DatabaseSample DataSee Sample Layer Item for sample data during Weather inactivity!Update FrequencyThe services is updated every 5 minutes using the Aggregated Live Feeds methodology.The overlay data is checked and updated daily from the official AWIPS Shapefile Database.Area CoveredUnited States and TerritoriesWhat can you do with this layer?Customize the display of each attribute by using the Change Style option for any layer.Query the layer to display only specific types of weather watches and warnings.Add to a map with other weather data layers to provide insight on hazardous weather events.Use ArcGIS Online analysis tools, such as Enrich Data, to determine the potential impact of weather events on populations.

Ecosystems are connected by flows of nutrients and organisms. Changes to connectivity and nutrient enrichment may destabilise ecosystem dynamics far from the nutrient source. We used gradostats to examine the effects of trophic connectivity (movement of consumers and producers) versus nutrient-only connectivity on the dynamics of Daphnia pulex (consumers) and algae (resources) in two metaecosystem configurations (linear vs. dendritic). We found that Daphnia peak population size and instability (coefficient of variation; CV) increased as distance from the nutrient input increased, but these effects were lower in metaecosystems connected by all trophic levels compared to nutrient-only connected systems and/or in dendritic compared to linear systems. We examined the effects of trophic connectivity (i.e. both trophic levels move rather than one or the other) using a generic model to qualitatively assess whether the expectations align with the ecosystem dynamics we observed. Analysis of our model shows that increased Daphnia population sizes and fluctuations in consumer-resource dynamics are expected with nutrient connectivity, with this pattern being more pronounced in linear rather than dendritic systems. These results confirm that connectivity may propagate and even amplify instability over a metaecosystem to communities distant from the source disturbance, and suggest a direction for future experiments, that recreate conditions closer to those found in natural systems.

Methods Our gradostat flasks contained simple communities of the water flea Daphnia pulex consuming a mix of three algal species (Pseudokirchneriella subcapitata, Scenedesmus quadricauda, Ankistrodesmus falcatus). This experiment employed a 2x2x2 factorial design to test the importance of ecosystem trophic connectivity (a treatment considering movement of medium only vs. movement of media, phytoplankton and Daphnia between flasks) and metaecosystem configuration (linear or dendritic) on the stability of Daphnia populations and algal communities with two levels of enriched medium input (regular and phosphorus-enriched). Four replicates of this whole design were established, for a total of 32 metaecosystems, run in 9 blocks due to time and space constraints. Each metaecosystem consisted of four “nodes” of 500 mL Erlenmeyer flasks with a foam stopper to allow for gas exchange (128 flasks total), seeded initially with 100 mL algal mix (total average algal density of 2.22 x106 +/- 1.3x104 cells/mL) to which 50 adult Daphnia with eggs (which produce broods of about 15 individuals each week in good conditions (Schwartz 1984) were added before topping off the flask to 500 mL with FLAMES media (Celis-Salgado et al. 2008). Configuration was controlled by unidirectionally connecting flasks in either a linear configuration (in →1→2→3→4→out) or a dendritic configuration (in →1, in→2, 1→3, 2→3, 3→4→out). We chose this as the simplest possible design in which a linear network could be compared to a branched network, with four nodes being the smallest possible number of nodes to create a dendritic configuration, and the two nodes branching into a third, similar to headwater in a river. Flasks were then connected by Tygon tubing and from an inflow reservoir of FLAMES medium (10 μgP/L) or enriched P (70 μgP /L) medium which was pumped through the array of flasks using peristaltic pumps (Watson-Marlow 503S/RL and Rainin Dynamix RP-1). Pumps were set on automatic timers to run for one hour each day at a speed adjusted to move a specific volume of media over that hour. The dilution rate was 10% of the total volume per for all flasks in the linear configurations and the “hub” (3) and “terminal” (4) nodes of the dendritic configurations (50 mL), and 5% per day (25 mL) for the “upstream” nodes in the dendritic configurations (Figure 1). We also controlled functional connectivity, contrasting metaecosystem dynamics when only nutrients moved versus the case when nutrients, resources and consumers moved. To block the flow of organisms in the nutrient-only connectivity treatment, outflow tubing was placed inside an 80-µm nylon mesh held in place with the stopper. Due to colony formation of the phytoplankton and clogging of the mesh, this proved to be an effective retention mechanism also for the algal resources, thus we believe flow of algae was significantly reduced in these treatments compared the trophic connectivity treatments. Though it is possible a small portion of single cells were able to pass through, Scenedesmus is known to form four-cell colonies in the presence of consumers (which we also observed in our algal counts), which are too large to pass through the mesh. As D. pulex were unable to fit through the tubing or survive moving through the peristaltic pumps, in the trophic connectivity treatment, D. pulex were manually moved using a 2mL transfer pipette at a rate of 10% of the population per day (20% were moved after each sampling count as sampling was only done every two days) in all linear nodes and the hub and terminal dendritic nodes, and 5% per day (10% moved after sampling) in the upstream dendritic nodes, in the same downstream direction as media. This type of passive movement at the flow rate of the system would be typical of planktonic animals in rivers that cannot swim upstream. Inflow stock solutions were prepared using FLAMES media (10 μgP/L). Finally, we modified our inflow reservoirs to contain either additionally P-enriched (high P) or regular (low P) FLAMES media. To increase P in the additionally nutrient-enriched treatment without changing pH, 132 μg/L of H2KPO4 and 168 μg/L H2KPO4 were added to our increased P treatment inflow stock solution. For the less-phosphorus treatment, no additional phosphorus was added, but 218 μg/L KCl were added to control for the K added to the high-P medium. See Figure S1 for a photograph of the experimental setup. Experimental Sampling The gradostats were sampled every other day for 30 days. In each node, the concentration of each algal species was measured using a haemocytometer. To estimate Daphnia population size, a 2mL plastic transfer pipette was used to gently agitate, and then sample each node. The number of individuals and two age classes (adult or juvenile) in the pipette were determined and then replaced to the experimental flask. This process was repeated five times, and the average D. pulex count of the five samples was used to estimate Daphnia density/2mL (total number estimated per flask = sampled count average *250). A pilot experiment testing this method proved it had an average error of 17.41 %, equating to 2.5 Daphnia more or less than the expected count at known densities; there is no reason to believe this error was systematic in one direction or the other, or to be systematically biased among our treatments. On Day 30 of the experiment, 40mL samples were taken from each flask to be analysed for total phosphorus concentration (TP). Phosphorus samples were analysed using a standard protocol (Wetzel and Likens 2013) at the GRIL-Université du Québec à Montréal analytical laboratory. Statistical Analysis To quantify the instability of Daphnia populations in experimental gradostats, we determined the peak total Dapnhia population size (as estimated by our density samples) and the coefficient of variation (CV) of Daphnia population size over the course of the experiment. These variables were calculated for each node within each gradostat, as well as in aggregate summed across all nodes for additive Daphnia metapopulation peak and CV. Similarly, population CV and peak density were calculated for each species of alga but we analyse here values based on total algal community density (sum of all species present), as Pseudorkirchinella and Ankistrodesmus were undetectable in most flasks for most of the experiment. Scenedesmus was mostly observed in 4-cell colonies, which is common in the presence of consumers, but we counted the total number of cells, not colonies. All analyses of experimental gradostat data were conducted in R version 4 (Team 2020). Statistical tests of the hypothesis were two-sided and with a level of significance of α=0.05. To determine whether metaecosystem connectivity, configuration and nutrient enrichment, as well as node position (1 upstream to 4 terminal), influenced node Daphnia population instability downstream of the nutrient enrichment source, we analysed the effects of these factors on mean Daphnia population and algal community peak values, on mean Daphnia population and algal community CV log-transformed (natural logarithm) values, and on mean final TP concentrations values, using linear mixed-effects models with the four factors as fixed effects. The mixed model included a random effect for ‘system’ which allowed us to account for a possible clustering in the response variables since the four nodes were connected as metaecosystems. For each of these models, pairwise interactions between factors were tested and terms for non-significant interactions were removed from the final models we report. Assumptions on the model errors (randomness, normality, and homoscedasticity) and the presence of possible influential observations or outliers were assessed with diagnostic plots of the model residuals. Robust standard errors (Huang and Li 2022) were used to adjust for heteroscedasticity. We also measured Daphnia metapopulation and algal metacommunity instability at the scale of the entire metaecosystem. To determine whether metaecosystem connectivity, configuration and nutrient enrichment influenced Daphnia metapopulation and algal metacommunity instability, we analysed the effects of these factors on mean Daphnia metapopulation and algal metacommunity peak values, and on mean CV values, using linear mixed-effects models with the three factors as fixed effects, using the block in which a metaecosystem was run

Establishment of a syndication flow from the department of Vendée (85) with the data of the “Hotels”, “Campings”, “Residences of holidays”, “Villages de vacances”, “Group accommodation”, “Rental accommodation” and “Rental areas” with enriched data: name, address, type of accommodation, means of communication (landline phone, e-mail and website), social networks, GPS coordinates, labels, languages spoken, payment methods accepted, opening dates, rates, details of visits, videos.

This feature service depicts the National Weather Service (NWS) watches, warnings, and advisories within the United States. Watches and warnings are classified into well over 100 categories. See event descriptions for full details. A warning is issued when a hazardous weather or hydrologic event is occurring, imminent or likely. A warning means weather conditions pose a threat to life or property. People in the path of the storm need to take protective action.A watch is used when the risk of a hazardous weather or hydrologic event has increased significantly, but its occurrence, location or timing is still uncertain. It is intended to provide enough lead time so those who need to set their plans in motion can do so. A watch means that hazardous weather is possible. People should have a plan of action in case a storm threatens, and they should listen for later information and possible warnings especially when planning travel or outdoor activities.An advisory is issued when a hazardous weather or hydrologic event is occurring, imminent or likely. Advisories are for less serious conditions than warnings, that cause significant inconvenience and if caution is not exercised, could lead to situations that may threaten life or property.SourceNational Weather Service RSS-CAP Warnings and Advisories: Public AlertsNational Weather Service Boundary Overlays: AWIPS Shapefile DatabaseSample DataSee Sample Layer Item for sample data during Weather inactivity!Update FrequencyThe services is updated every 5 minutes using the Aggregated Live Feeds methodology.The overlay data is checked and updated daily from the official AWIPS Shapefile Database.Area CoveredUnited States and TerritoriesWhat can you do with this layer?Customize the display of each attribute by using the Change Style option for any layer.Query the layer to display only specific types of weather watches and warnings.Add to a map with other weather data layers to provide insight on hazardous weather events.Use ArcGIS Online analysis tools, such as Enrich Data, to determine the potential impact of weather events on populations.Revisions:Feb 25, 2021: Revised service data upate workflow, improving stability and update interval.Process now checks for data updates every 5 minutes!Mar 3, 2021: Revised data processing to leverage VTEC parameter details to better align Event 'effective' dates with reported dates on Alert pages.Apr 17, 2023: Turned off popups for boundary Layers by default.Feb 1, 2024: Revised to leverage CAP v1.2 source endpoint. Update event link to use alert search.Feb 16, 2024: Revised event link to accomodate change in alert search endpoint.Jan 19, 2025: Added event 'Description' and 'Instructions', updated Pop-up.Jan 22, 2025: Exposed 'Hours Old' fields supporting last 'Updated', 'Effective', and 'Expiration' as +- age values for events.This map is provided for informational purposes and is not monitored 24/7 for accuracy and currency.If you would like to be alerted to potential issues or simply see when this Service will update next, please visit our Live Feed Status Page!

Arbuscular mycorrhizal fungi (AMF) facilitate ecosystem functioning through provision of plant hosts with phosphorus (P), especially where soil P is limiting. Changes in soil nutrient regimes are expected to impact AMF, but the direction of the impact may depend on context. We predicted that nitrogen (N)-only enrichment promotes plant invasions and exacerbates their P limitation, increasing the utility of AMF and promoting AMF diversity. We expected that enrichment with N, P and other nutrients similarly promotes plant invasions, but decreases the benefit and diversity of AMF because P is readily available for both native and exotic plants. We tested these hypotheses in eucalypt woodlands of south-western Australia, that occur on soils naturally low in P. We evaluated AMF communities within three modified ground-layer states representing different types of nutrient enrichment and associated plant invasions. We compared these modified states to near-natural reference woodlands. AMF richness varied across ground-layer states. The moderately invaded/N-enriched state showed the highest AMF richness, while the highly invaded/NP-enriched state showed the lowest AMF richness. The reference state and the weakly invaded/enriched state were intermediate. AMF richness and colonisation were higher in roots of exotic than native plant species. AMF community composition differed among ground-layer states, with the highly invaded/NP-enriched state being most distinct. Distinctions among states were often driven by family-level patterns. Reference and moderately invaded/N-enriched states each supported distinct groups of zero-radius operational taxonomic units (zOTUs) in Acaulosporaceae, Gigasporaceae and Glomeraceae, whereas Gigasporaceae and Glomeraceae were nearly absent from the highly invaded/NP-enriched state. Further, Diversisporaceae and Glomeraceae were most diverse in the moderately invaded/N-enriched state. Synthesis. Both the nature of soil nutrient enrichment and plant provenance matter for AMF. N-only enrichment of low-P soils increased AMF richness, likely due to introduction of AMF-dependent exotic plant species and exacerbation of their P-limitation. In contrast, multi-nutrient enrichment, decreased AMF richness potentially due to a decrease in host dependence on AMF, regardless of host provenance. The changes in AMF community composition with nutrient enrichment and plant invasion warrants further research into predicting the functional implications of these changes. Methods Site selection Sampling was centred on two nature reserves with areas of York gum (Eucalyptus loxophleba subsp. loxophleba) – Jam (Acacia acuminata) woodlands (hereafter York gum woodlands) in close to reference condition. The reserves are located in the central wheatbelt, Western Australia: Mount Caroline (31°45'25.3"S, 117°38'38.3"E) and Namelkatchem Nature Reserves (31°10’47.9”S, 117°11’18.1”E), and are ~ 70 km apart (Fig. 1). These reserves have a history of minimal livestock grazing and plant invasion, permitting the persistence of large areas of diverse understoreys dominated by native perennial and annual forbs and grasses (Prober and Wiehl 2012). The climate is Mediterranean type, with long-term (1990–2022) mean annual temperature and rainfall, respectively, of 17.8 °C and 321 mm at Mt Caroline and 17.6 °C and 333 mm at Namelkatchem (BoM, 2023). Our experimental design involved four ground-layer states representing different levels of nutrient enrichment and plant invasions as described above: a reference (control) state in near natural conditions with naturally low soil P and N, and three degraded states that we sought from different parts of the landscape: a weakly invaded/enriched state, a moderately invaded/N-enriched, and a highly invaded/NP-enriched state (Table 1; Fig. 1). We chose the term ‘ground-layer state’ to be consistent with previous research of this ecosystem (Prober et al. 2012). ‘Ground-layer’ is used rather than ‘understorey’ to distinguish the herbaceous ground-layer from a shrub layer that can occur in the woodland understorey. Two areas within each of the two nature reserves with few-to-no exotic plant species, were selected to sample the ‘reference’ state. Each reserve included localised patches invaded by exotic annuals, that likely arose historically due to localised disturbances (e.g. by introduced rabbits). These patches were used to represent the ‘moderately invaded/N-enriched state’, given that other studies have demonstrated that such exotic-invaded states of York gum woodlands are typically N-enriched (Prober and Wiehl, 2012). Because P-enriched areas rarely occur inside the nature reserves, to represent the ‘highly invaded/NP-enriched’ state we sampled four fertilised 2 m × 2 m plots from a pre-existing long-term nutrient addition experiment located ~1.5 km from Mount Caroline Nature reserve (31°46'56.43"S, 117°36'41.61"E; Fig. 1). The experiment was established in a grazed York gum woodland remnant in 2009, as part of the global Nutrient Network (NutNet) experiment (Borer et al. 2014). We also sampled four unfertilised plots from the same experiment, representing the ‘weakly invaded/enriched’ state that arose through historical sheep grazing, resulting in some plant invasion and N and P enrichment. To match the NutNet design, plots of 2 m × 2 m were established at both nature reserves for the reference and moderately invaded/N-enriched states. Plots were at least 20 m apart, with four replicate plots of each ground-layer state. Remnants of York gum woodlands are rare in the landscape; hence we chose the two closest nature reserves near the NutNet experiment. This was done to incorporate as much of the natural variation within Reference sites as possible. We note that a previous study found little variation in AMF communities among remnants of York gum woodlands across 200 km distance (Prober et al. 2015). The experimental plots from NutNet were arranged in a randomised complete block design with four blocks. Nutrients were added annually in autumn to plots representing the highly invaded/NP-enriched state, as 10 g N per m2·yr−1 of timed-release urea, 10 g P per m2·yr−1 as triple superphosphate, and 10 g K per m2·y−1 as potassium sulphate. These plots also received a once-off addition of other macro- and micronutrients in 2009: 100 g per m−2 of a mix containing iron (15%), sulphur (14%), magnesium (1.5%), manganese (2.5%), copper (1%), zinc (1%), boron (0.2%), and molybdenum (0.05%). Weakly invaded/enriched plots were dominated by native plant species and highly invaded/NP-enriched plots were dominated by exotic plant species. The experimental plots had been open to livestock grazing since European colonisation (1860s) until 2015. Sample collection and processing
Sampling occurred in August 2021 during the growing season. In each plot, plant community composition and cover were recorded. Then, rhizosphere soil and roots were collected from two plants of the six most abundant plant species. Samples from the two plants per species were pooled for a total of six samples per plot. Soils were thoroughly mixed in a sealable bag, collecting a subsample for DNA analyses. Roots were immediately stored in 98% ethanol pending processing. Soil and root samples were stored in 15 ml tubes and placed in dry ice during sampling and transport to the laboratory. Samples were stored at -80°C thereafter. Root samples were thoroughly cleaned with deionised water, and fine roots of < 2 mm were retained. Clean root samples were split in two: one part for DNA analyses and one for root colonisation assessment. Two plots had only five plant species, resulting in 94 soil and 94 root samples (Table S1). No permit was needed to access and sample plots from the NutNet experiment. Permits to access and sample the two nature reserves were granted by the Department of Biodiversity, Conservation and Attractions of Western Australia (Licence: FT61000839; Regulation 4: CE006388). Soil chemical analyses Soil samples were sent to CSBP Laboratories (Bibra Lake, Western Australia) for nutrient analyses. Plant-available P and K were measured using the Colwell test (Colwell, 1963; Rayment & Higginson, 1992). Organic carbon (OC) was determined according to Walkley & Black (1934). Ammonium-N, nitrate-N, and total N were measured as per Searle (1984). Soil pH was measured in CaCl2 in a solution ratio of 1:5 (Rayment & Lyons, 2012). Root colonisation Root subsamples allocated for measurement of root colonisation were cleared in 1 M KOH and stained with ink in vinegar (5% v/v) as described by Vierheilig et al. (1998). Colonization by AMF, including Glomeromycotina-AMF and Mucoromycotina-AMF, was scored using the line intercept method (McGonigle et al. 1990). One hundred intercept points were scored for each sample, and the percentage of root length colonized by AMF was calculated. DNA extraction and sequencing Root samples allocated for DNA analyses were cut into 5 mm pieces, homogenised, and ground with beads. DNA was extracted from 20 mg of root and 250 mg of soil material using the DNeasy Plant Pro kit and DNease PowerSoil Pro kit, respectively, (Qiagen, Carlsbad, USA). PCR amplification and sequencing was performed by the Australian Genome Research Facility. For each sample, 15 ng DNA were used to amplify the 18S rRNA gene using the AMF primer set AMV4.5NF and AMDGR (Sato et al., 2005). These primers accurately retrieve a wide range of AMF taxa, including both Glomeromycotina and Mucoromycotina subphyla (Orchard et al., 2017; Albornoz et al., 2022). Thermocycling was completed with an Applied Biosystem 384 Veriti and using Platinum SuperFi II mastermix (Life Technologies, Australia) for the primary PCR. Thermocycling consisted of an initial denaturation at 98℃ for 30 s followed by 30 cycles of 98℃ for 10 s, 60℃ for 10 s and 72℃ for 30 s. The final extension was

This feature service depicts the National Weather Service (NWS) watches, warnings, and advisories within the United States. Watches and warnings are classified into well over 100 categories. See event descriptions for full details. A warning is issued when a hazardous weather or hydrologic event is occurring, imminent or likely. A warning means weather conditions pose a threat to life or property. People in the path of the storm need to take protective action.A watch is used when the risk of a hazardous weather or hydrologic event has increased significantly, but its occurrence, location or timing is still uncertain. It is intended to provide enough lead time so those who need to set their plans in motion can do so. A watch means that hazardous weather is possible. People should have a plan of action in case a storm threatens, and they should listen for later information and possible warnings especially when planning travel or outdoor activities.An advisory is issued when a hazardous weather or hydrologic event is occurring, imminent or likely. Advisories are for less serious conditions than warnings, that cause significant inconvenience and if caution is not exercised, could lead to situations that may threaten life or property.SourceNational Weather Service RSS-CAP Warnings and Advisories: Public AlertsNational Weather Service Boundary Overlays: AWIPS Shapefile DatabaseSample DataSee Sample Layer Item for sample data during Weather inactivity!Update FrequencyThe services is updated every 5 minutes using the Aggregated Live Feeds methodology.The overlay data is checked and updated daily from the official AWIPS Shapefile Database.Area CoveredUnited States and TerritoriesWhat can you do with this layer?Customize the display of each attribute by using the Change Style option for any layer.Query the layer to display only specific types of weather watches and warnings.Add to a map with other weather data layers to provide insight on hazardous weather events.Use ArcGIS Online analysis tools, such as Enrich Data, to determine the potential impact of weather events on populations.Revisions:Feb 25, 2021: Revised service data upate workflow, improving stability and update interval.Process now checks for data updates every 5 minutes!Mar 3, 2021: Revised data processing to leverage VTEC parameter details to better align Event 'effective' dates with reported dates on Alert pages.Apr 17, 2023: Turned off popups for boundary Layers by default.Feb 1, 2024: Revised to leverage CAP v1.2 source endpoint. Update event link to use alert search.Feb 16, 2024: Revised event link to accomodate change in alert search endpoint.Jan 19, 2025: Added event 'Description' and 'Instructions', updated Pop-up.Jan 22, 2025: Exposed 'Hours Old' fields supporting last 'Updated', 'Effective', and 'Expiration' as +- age values for events.This map is provided for informational purposes and is not monitored 24/7 for accuracy and currency.If you would like to be alerted to potential issues or simply see when this Service will update next, please visit our Live Feed Status Page!

This feature service depicts the National Weather Service (NWS) watches, warnings, and advisories within the United States. Watches and warnings are classified into 43 categories.A warning is issued when a hazardous weather or hydrologic event is occurring, imminent or likely. A warning means weather conditions pose a threat to life or property. People in the path of the storm need to take protective action.A watch is used when the risk of a hazardous weather or hydrologic event has increased significantly, but its occurrence, location or timing is still uncertain. It is intended to provide enough lead time so those who need to set their plans in motion can do so. A watch means that hazardous weather is possible. People should have a plan of action in case a storm threatens, and they should listen for later information and possible warnings especially when planning travel or outdoor activities.An advisory is issued when a hazardous weather or hydrologic event is occurring, imminent or likely. Advisories are for less serious conditions than warnings, that cause significant inconvenience and if caution is not exercised, could lead to situations that may threaten life or property.SourceNational Weather Service RSS-CAP Warnings and Advisories: Public AlertsNational Weather Service Boundary Overlays: AWIPS Shapefile DatabaseSample DataSee Sample Layer Item for sample data during Weather inactivity!Update FrequencyThe services is updated every 5 minutes using the Aggregated Live Feeds methodology.The overlay data is checked and updated daily from the official AWIPS Shapefile Database.Area CoveredUnited States and TerritoriesWhat can you do with this layer?Customize the display of each attribute by using the Change Style option for any layer.Query the layer to display only specific types of weather watches and warnings.Add to a map with other weather data layers to provide insight on hazardous weather events.Use ArcGIS Online analysis tools, such as Enrich Data, to determine the potential impact of weather events on populations.This map is provided for informational purposes and is not monitored 24/7 for accuracy and currency.Additional information on Watches and Warnings.

Supplementary materials (Appendix A and B) for the article:

Traffic Information Enrichment: Creating Long-Term Traffic Speed Prediction Ensemble Model for Better Navigation through Waypoints

Abstract: Traffic speed prediction for a selected road segment from a short-term and long-term perspective is among the fundamental issues of intelligent transportation systems (ITS). During the course of the past two decades, many artefacts (e.g., models) have been designed dealing with traffic speed prediction. However, no satisfactory solution has been found for the issue of a long-term prediction for days and weeks using the vast spatial and temporal data. This article aims to introduce a long-term traffic speed prediction ensemble model using country-scale historic traffic data from 37,002 km of roads, which constitutes 66% of all roads in the Czech Republic. The designed model comprises three submodels and combines parametric and nonparametric approaches in order to acquire a good-quality prediction that can enrich available real-time traffic information. Furthermore, the model is set into a conceptual design which expects its usage for the improvement of navigation through waypoints (e.g., delivery service, goods distribution, police patrol) and the estimated arrival time. The model validation is carried out using the same network of roads, and the model predicts traffic speed in the period of 1 week. According to the performed validation of average speed prediction at a given hour, it can be stated that the designed model achieves good results, with mean absolute error of 4.67 km/h. The achieved results indicate that the designed solution can effectively predict the long-term speed information using large-scale spatial and temporal data, and that this solution is suitable for use in ITS.

Simunek, M., & Smutny, Z. (2021). Traffic Information Enrichment: Creating Long-Term Traffic Speed Prediction Ensemble Model for Better Navigation through Waypoints. Applied Sciences, 11(1), 315. https://doi.org/10.3390/app11010315

Appendix A Examples of the deviation between the average speed and the FreeFlowSpeed for selected hours.

Appendix B The text file provides a complete overview of all road segments on which basis summary test results were calculated in Section 6 of the article.

Highly complex and dynamic protein mixtures are hardly comprehensively resolved by direct shotgun proteomic analysis. As many proteins of biological interest are of low abundance, numerous analytical methodologies have been developed to reduce sample complexity and go deeper into proteomes. The present work describes an analytical strategy to perform cysteinyl-peptide subset enrichment and relative quantification through successive cysteine and amine-isobaric tagging. A cysteine-reactive covalent capture tag (C3T) allowed derivatization of cysteines and specific isolation on a covalent capture (CC) resin. The 6-plex amine-reactive tandem mass tags (TMT) served for relative quantification of the targeted peptides. The strategy was first evaluated on a model protein mixture with increasing concentrations to assess the specificity of the enrichment and the quantitative performances of the workflow. It was then applied to human cerebrospinal fluid (CSF) from post-mortem and ante-mortem samples. These studies confirmed the specificity of the C3T and the CC technique to cysteine-containing peptides. The model protein mixture analysis showed high precision and accuracy of the quantification with coefficients of variation and mean absolute errors of less than 10% on average. The CSF experiments demonstrated the potential of the strategy to study complex biological samples and identify differential brain-related proteins. In addition, the quantification data were highly correlated with a classical TMT experiment (i.e., without C3T cysteine-tagging and enrichment steps). Altogether, these results legitimate the use of this quantitative C3T strategy to enrich and relatively quantify cysteine-containing peptides in complex mixtures.