3 datasets found
  1. Data from: X-ray CT data with semantic annotations for the paper "A workflow...

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    Updated May 2, 2024
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    Agricultural Research Service (2024). X-ray CT data with semantic annotations for the paper "A workflow for segmenting soil and plant X-ray CT images with deep learning in Google’s Colaboratory" [Dataset]. https://catalog.data.gov/dataset/x-ray-ct-data-with-semantic-annotations-for-the-paper-a-workflow-for-segmenting-soil-and-p-d195a
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    Dataset updated
    May 2, 2024
    Dataset provided by
    Agricultural Research Servicehttps://www.ars.usda.gov/
    Description

    Leaves from genetically unique Juglans regia plants were scanned using X-ray micro-computed tomography (microCT) on the X-ray μCT beamline (8.3.2) at the Advanced Light Source (ALS) in Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA USA). Soil samples were collected in Fall of 2017 from the riparian oak forest located at the Russell Ranch Sustainable Agricultural Institute at the University of California Davis. The soil was sieved through a 2 mm mesh and was air dried before imaging. A single soil aggregate was scanned at 23 keV using the 10x objective lens with a pixel resolution of 650 nanometers on beamline 8.3.2 at the ALS. Additionally, a drought stressed almond flower bud (Prunus dulcis) from a plant housed at the University of California, Davis, was scanned using a 4x lens with a pixel resolution of 1.72 µm on beamline 8.3.2 at the ALS Raw tomographic image data was reconstructed using TomoPy. Reconstructions were converted to 8-bit tif or png format using ImageJ or the PIL package in Python before further processing. Images were annotated using Intel’s Computer Vision Annotation Tool (CVAT) and ImageJ. Both CVAT and ImageJ are free to use and open source. Leaf images were annotated in following Théroux-Rancourt et al. (2020). Specifically, Hand labeling was done directly in ImageJ by drawing around each tissue; with 5 images annotated per leaf. Care was taken to cover a range of anatomical variation to help improve the generalizability of the models to other leaves. All slices were labeled by Dr. Mina Momayyezi and Fiona Duong.To annotate the flower bud and soil aggregate, images were imported into CVAT. The exterior border of the bud (i.e. bud scales) and flower were annotated in CVAT and exported as masks. Similarly, the exterior of the soil aggregate and particulate organic matter identified by eye were annotated in CVAT and exported as masks. To annotate air spaces in both the bud and soil aggregate, images were imported into ImageJ. A gaussian blur was applied to the image to decrease noise and then the air space was segmented using thresholding. After applying the threshold, the selected air space region was converted to a binary image with white representing the air space and black representing everything else. This binary image was overlaid upon the original image and the air space within the flower bud and aggregate was selected using the “free hand” tool. Air space outside of the region of interest for both image sets was eliminated. The quality of the air space annotation was then visually inspected for accuracy against the underlying original image; incomplete annotations were corrected using the brush or pencil tool to paint missing air space white and incorrectly identified air space black. Once the annotation was satisfactorily corrected, the binary image of the air space was saved. Finally, the annotations of the bud and flower or aggregate and organic matter were opened in ImageJ and the associated air space mask was overlaid on top of them forming a three-layer mask suitable for training the fully convolutional network. All labeling of the soil aggregate and soil aggregate images was done by Dr. Devin Rippner. These images and annotations are for training deep learning models to identify different constituents in leaves, almond buds, and soil aggregates Limitations: For the walnut leaves, some tissues (stomata, etc.) are not labeled and only represent a small portion of a full leaf. Similarly, both the almond bud and the aggregate represent just one single sample of each. The bud tissues are only divided up into buds scales, flower, and air space. Many other tissues remain unlabeled. For the soil aggregate annotated labels are done by eye with no actual chemical information. Therefore particulate organic matter identification may be incorrect. Resources in this dataset:Resource Title: Annotated X-ray CT images and masks of a Forest Soil Aggregate. File Name: forest_soil_images_masks_for_testing_training.zipResource Description: This aggregate was collected from the riparian oak forest at the Russell Ranch Sustainable Agricultural Facility. The aggreagate was scanned using X-ray micro-computed tomography (microCT) on the X-ray μCT beamline (8.3.2) at the Advanced Light Source (ALS) in Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA USA) using the 10x objective lens with a pixel resolution of 650 nanometers. For masks, the background has a value of 0,0,0; pores spaces have a value of 250,250, 250; mineral solids have a value= 128,0,0; and particulate organic matter has a value of = 000,128,000. These files were used for training a model to segment the forest soil aggregate and for testing the accuracy, precision, recall, and f1 score of the model.Resource Title: Annotated X-ray CT images and masks of an Almond bud (P. Dulcis). File Name: Almond_bud_tube_D_P6_training_testing_images_and_masks.zipResource Description: Drought stressed almond flower bud (Prunis dulcis) from a plant housed at the University of California, Davis, was scanned by X-ray micro-computed tomography (microCT) on the X-ray μCT beamline (8.3.2) at the Advanced Light Source (ALS) in Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA USA) using the 4x lens with a pixel resolution of 1.72 µm using. For masks, the background has a value of 0,0,0; air spaces have a value of 255,255, 255; bud scales have a value= 128,0,0; and flower tissues have a value of = 000,128,000. These files were used for training a model to segment the almond bud and for testing the accuracy, precision, recall, and f1 score of the model.Resource Software Recommended: Fiji (ImageJ),url: https://imagej.net/software/fiji/downloads Resource Title: Annotated X-ray CT images and masks of Walnut leaves (J. Regia) . File Name: 6_leaf_training_testing_images_and_masks_for_paper.zipResource Description: Stems were collected from genetically unique J. regia accessions at the 117 USDA-ARS-NCGR in Wolfskill Experimental Orchard, Winters, California USA to use as scion, and were grafted by Sierra Gold Nursery onto a commonly used commercial rootstock, RX1 (J. microcarpa × J. regia). We used a common rootstock to eliminate any own-root effects and to simulate conditions for a commercial walnut orchard setting, where rootstocks are commonly used. The grafted saplings were repotted and transferred to the Armstrong lathe house facility at the University of California, Davis in June 2019, and kept under natural light and temperature. Leaves from each accession and treatment were scanned using X-ray micro-computed tomography (microCT) on the X-ray μCT beamline (8.3.2) at the Advanced Light Source (ALS) in Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA USA) using the 10x objective lens with a pixel resolution of 650 nanometers. For masks, the background has a value of 170,170,170; Epidermis value= 85,85,85; Mesophyll value= 0,0,0; Bundle Sheath Extension value= 152,152,152; Vein value= 220,220,220; Air value = 255,255,255.Resource Software Recommended: Fiji (ImageJ),url: https://imagej.net/software/fiji/downloads

  2. Labeled high-resolution orthoimagery time-series of an alluvial river...

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    Updated Nov 20, 2023
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    Daniel Buscombe; Daniel Buscombe (2023). Labeled high-resolution orthoimagery time-series of an alluvial river corridor; Elwha River, Washington, USA. [Dataset]. http://doi.org/10.5281/zenodo.10155783
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    zipAvailable download formats
    Dataset updated
    Nov 20, 2023
    Dataset provided by
    Zenodohttp://zenodo.org/
    Authors
    Daniel Buscombe; Daniel Buscombe
    License

    Attribution 4.0 (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/
    License information was derived automatically

    Time period covered
    Nov 18, 2023
    Area covered
    Elwha River, United States, Washington
    Description

    Labeled high-resolution orthoimagery time-series of an alluvial river corridor; Elwha River, Washington, USA.

    Daniel Buscombe, Marda Science LLC

    There are two datasets in this data release:

    1. Model training dataset. A manually (or semi-manually) labeled image dataset that was used to train and evaluate a machine (deep) learning model designed to identify subaerial accumulations of large wood, alluvial sediment, water, and vegetation in orthoimagery of alluvial river corridors in forested catchments.

    2. Model output dataset. A labeled image dataset that uses the aforementioned model to estimate subaerial accumulations of large wood, alluvial sediment, water, and vegetation in a larger orthoimagery dataset of alluvial river corridors in forested catchments.

    All of these label data are derived from raw gridded data that originate from the U.S. Geological Survey (Ritchie et al., 2018). That dataset consists of 14 orthoimages of the Middle Reach (MR, in between the former Aldwell and Mills reservoirs) and 14 corresponding Lower Reach (LR, downstream of the former Mills reservoir) of the Elwha River, Washington, collected between the period 2012-04-07 and 2017-09-22. That orthoimagery was generated using SfM photogrammetry (following Over et al., 2021) using a photographic camera mounted to an aircraft wing. The imagery capture channel change as it evolved under a ~20 Mt sediment pulse initiated by the removal of the two dams. The two reaches are the ~8 km long Middle Reach (MR) and the lower-gradient ~7 km long Lower Reach (LR).

    The orthoimagery have been labeled (pixelwise, either manually or by an automated process) according to the following classes (inter class in the label data in parentheses):

    1. vegetation / other (0)

    2. water (1)

    3. sediment (2)

    4. large wood (3)

    1. Model training dataset.

    Imagery was labeled using a combination of the open-source software Doodler (Buscombe et al., 2021; https://github.com/Doodleverse/dash_doodler) and hand-digitization using QGIS at 1:300 scale, rasterizeing the polygons, and gridded and clipped in the same way as all other gridded data. Doodler facilitates relatively labor-free dense multiclass labeling of natural imagery, enabling relatively rapid training dataset creation. The final training dataset consists of 4382 images and corresponding labels, each 1024 x 1024 pixels and representing just over 5% of the total data set. The training data are sampled approximately equally in time and in space among both reaches. All training and validation samples purposefully included all four label classes, to avoid model training and evaluation problems associated with class imbalance (Buscombe and Goldstein, 2022).

    Data are provided in geoTIFF format. The imagery and label grids (imagery) are reprojected to be co-located in the NAD83(2011) / UTM zone 10N projection, and to consist of 0.125 x 0.125m pixels.

    Pixel-wise labels measurements such as these facilitate development and evaluation of image segmentation, image classification, object-based image-analysis (OBIA), and object-in-image detection models, and numerous potential other machine learning models for the general purposes of river corridor classification, description, enumeration, inventory, and process or state quantification. For example this dataset may serve in transfer learning contexts for application in different river or coastal environments or for different tasks or class ontologies.

    Files:

    1. Labels_used_for_model_training_Buscombe_Labeled_high_resolution_orthoimagery_time_series_of_an_alluvial_river_corridor_Elwha_River_Washington_USA.zip, 63 MB, label tiffs

    2. Model_training_ images1of4.zip, 1.5 GB, imagery tiffs

    3. Model_training_ images2of4.zip, 1.5 GB, imagery tiffs

    4. Model_training_ images3of4.zip, 1.7 GB, imagery tiffs

    5. Model_training_ images4of4.zip, 1.6 GB, imagery tiffs

    2. Model output dataset.

    Imagery was labeled using a deep-learning based semantic segmentation model (Buscombe, 2023) trained specifically for the task using the Segmentation Gym (Buscombe and Goldstein, 2022) modeling suite. We use the software package Segmentation Gym (Buscombe and Goldstein, 2022) to fine-tune a Segformer (Xie et al., 2021) deep learning model for semantic image segmentation. We take the instance (i.e. model architecture and trained weights) of the model of Xie et al. (2021), itself fine-tuned on ADE20k dataset (Zhou et al., 2019) at resolution 512x512 pixels, and fine-tune it on our 1024x1024 pixel training data consisting of 4-class label images.

    The spatial extent of the imagery in the MR is 455157.2494695878122002,5316532.9804129302501678 : 457076.1244695878122002,5323771.7304129302501678. Imagery width is 15351 pixels and imagery height is 57910 pixels. The spatial extent of the imagery in the LR is 457704.9227139975992031,5326631.3750646486878395 : 459241.6727139975992031,5333311.0000646486878395. Imagery width is 12294 pixels and imagery height is 53437 pixels. Data are provided in Cloud-Optimzed geoTIFF (COG) format. The imagery and label grids (imagery) are reprojected to be co-located in the NAD83(2011) / UTM zone 10N projection, and to consist of 0.125 x 0.125m pixels. All grids have been clipped to the union of extents of active channel margins during the period of interest.

    Reach-wide pixel-wise measurements such as these facilitate comparison of wood and sediment storage at any scale or location. These data may be useful for studying the morphodynamics of wood-sediment interactions in other geomorphically complex channels, wood storage in channels, the role of wood in ecosystems and conservation or restoration efforts.

    Files:

    1. Elwha_MR_labels_Buscombe_Labeled_high_resolution_orthoimagery_time_series_of_an_alluvial_river_corridor_Elwha_River_Washington_USA.zip, 9.67 MB, label COGs from Elwha River Middle Reach (MR)

    2. ElwhaMR_ imagery_ part1_ of 2.zip, 566 MB, imagery COGs from Elwha River Middle Reach (MR)

    3. ElwhaMR imagery_ part2_ of_ 2.zip, 618 MB, imagery COGs from Elwha River Middle Reach (MR)

    3. Elwha_LR_labels_Buscombe_Labeled_high_resolution_orthoimagery_time_series_of_an_alluvial_river_corridor_Elwha_River_Washington_USA.zip, 10.96 MB, label COGs from Elwha River Lower Reach (LR)

    4. ElwhaLR_ imagery_ part1_ of 2.zip, 622 MB, imagery COGs from Elwha River Middle Reach (MR)

    5. ElwhaLR imagery_ part2_ of_ 2.zip, 617 MB, imagery COGs from Elwha River Middle Reach (MR)

    This dataset was created using open-source tools of the Doodleverse, a software ecosystem for geoscientific image segmentation, by Daniel Buscombe (https://github.com/dbuscombe-usgs) and Evan Goldstein (https://github.com/ebgoldstein). Thanks to the contributors of the Doodleverse!. Thanks especially Sharon Fitzpatrick (https://github.com/2320sharon) and Jaycee Favela for contributing labels.

    References

    • Buscombe, D. (2023). Doodleverse/Segmentation Gym SegFormer models for 4-class (other, water, sediment, wood) segmentation of RGB aerial orthomosaic imagery (v1.0) [Data set]. Zenodo. https://doi.org/10.5281/zenodo.8172858

    • Buscombe, D., Goldstein, E. B., Sherwood, C. R., Bodine, C., Brown, J. A., Favela, J., et al. (2021). Human-in-the-loop segmentation of Earth surface imagery. Earth and Space Science, 9, e2021EA002085. https://doi.org/10.1029/2021EA002085

    • Buscombe, D., & Goldstein, E. B. (2022). A reproducible and reusable pipeline for segmentation of geoscientific imagery. Earth and Space Science, 9, e2022EA002332. https://doi.org/10.1029/2022EA002332 See: https://github.com/Doodleverse/segmentation_gym

    • Over, J.R., Ritchie, A.C., Kranenburg, C.J., Brown, J.A., Buscombe, D., Noble, T., Sherwood, C.R., Warrick, J.A., and Wernette, P.A., 2021, Processing coastal imagery with Agisoft Metashape Professional Edition, version 1.6—Structure from motion workflow documentation: U.S. Geological Survey Open-File Report 2021–1039, 46 p., https://doi.org/10.3133/ofr20211039.

    • Ritchie, A.C., Curran, C.A., Magirl, C.S., Bountry, J.A., Hilldale, R.C., Randle, T.J., and Duda, J.J., 2018, Data in support of 5-year sediment budget and morphodynamic analysis of Elwha River following dam removals: U.S. Geological Survey data release, https://doi.org/10.5066/F7PG1QWC.

    • Xie, E., Wang, W., Yu, Z., Anandkumar, A., Alvarez, J.M. and Luo, P., 2021. SegFormer: Simple and efficient design for semantic segmentation with transformers. Advances in Neural Information Processing Systems, 34, pp.12077-12090.

    • Zhou, B., Zhao, H., Puig, X., Xiao, T., Fidler, S., Barriuso, A. and Torralba, A., 2019. Semantic understanding of scenes through the ade20k dataset. International Journal of Computer Vision, 127, pp.302-321.


  3. TreeSatAI Benchmark Archive for Deep Learning in Forest Applications

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    • data.niaid.nih.gov
    bin, pdf, zip
    Updated Jul 16, 2024
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    Christian Schulz; Christian Schulz; Steve Ahlswede; Steve Ahlswede; Christiano Gava; Patrick Helber; Patrick Helber; Benjamin Bischke; Benjamin Bischke; Florencia Arias; Michael Förster; Michael Förster; Jörn Hees; Jörn Hees; Begüm Demir; Begüm Demir; Birgit Kleinschmit; Birgit Kleinschmit; Christiano Gava; Florencia Arias (2024). TreeSatAI Benchmark Archive for Deep Learning in Forest Applications [Dataset]. http://doi.org/10.5281/zenodo.6598391
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    pdf, zip, binAvailable download formats
    Dataset updated
    Jul 16, 2024
    Dataset provided by
    Zenodohttp://zenodo.org/
    Authors
    Christian Schulz; Christian Schulz; Steve Ahlswede; Steve Ahlswede; Christiano Gava; Patrick Helber; Patrick Helber; Benjamin Bischke; Benjamin Bischke; Florencia Arias; Michael Förster; Michael Förster; Jörn Hees; Jörn Hees; Begüm Demir; Begüm Demir; Birgit Kleinschmit; Birgit Kleinschmit; Christiano Gava; Florencia Arias
    License

    Attribution 4.0 (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/
    License information was derived automatically

    Description

    Context and Aim

    Deep learning in Earth Observation requires large image archives with highly reliable labels for model training and testing. However, a preferable quality standard for forest applications in Europe has not yet been determined. The TreeSatAI consortium investigated numerous sources for annotated datasets as an alternative to manually labeled training datasets.

    We found the federal forest inventory of Lower Saxony, Germany represents an unseen treasure of annotated samples for training data generation. The respective 20-cm Color-infrared (CIR) imagery, which is used for forestry management through visual interpretation, constitutes an excellent baseline for deep learning tasks such as image segmentation and classification.

    Description

    The data archive is highly suitable for benchmarking as it represents the real-world data situation of many German forest management services. One the one hand, it has a high number of samples which are supported by the high-resolution aerial imagery. On the other hand, this data archive presents challenges, including class label imbalances between the different forest stand types.

    The TreeSatAI Benchmark Archive contains:

    • 50,381 image triplets (aerial, Sentinel-1, Sentinel-2)

    • synchronized time steps and locations

    • all original spectral bands/polarizations from the sensors

    • 20 species classes (single labels)

    • 12 age classes (single labels)

    • 15 genus classes (multi labels)

    • 60 m and 200 m patches

    • fixed split for train (90%) and test (10%) data

    • additional single labels such as English species name, genus, forest stand type, foliage type, land cover

    The geoTIFF and GeoJSON files are readable in any GIS software, such as QGIS. For further information, we refer to the PDF document in the archive and publications in the reference section.

    Version history

    v1.0.0 - First release

    Citation

    Ahlswede et al. (in prep.)

    GitHub

    Full code examples and pre-trained models from the dataset article (Ahlswede et al. 2022) using the TreeSatAI Benchmark Archive are published on the GitHub repositories of the Remote Sensing Image Analysis (RSiM) Group (https://git.tu-berlin.de/rsim/treesat_benchmark). Code examples for the sampling strategy can be made available by Christian Schulz via email request.

    Folder structure

    We refer to the proposed folder structure in the PDF file.

    • Folder “aerial” contains the aerial imagery patches derived from summertime orthophotos of the years 2011 to 2020. Patches are available in 60 x 60 m (304 x 304 pixels). Band order is near-infrared, red, green, and blue. Spatial resolution is 20 cm.

    • Folder “s1” contains the Sentinel-1 imagery patches derived from summertime mosaics of the years 2015 to 2020. Patches are available in 60 x 60 m (6 x 6 pixels) and 200 x 200 m (20 x 20 pixels). Band order is VV, VH, and VV/VH ratio. Spatial resolution is 10 m.

    • Folder “s2” contains the Sentinel-2 imagery patches derived from summertime mosaics of the years 2015 to 2020. Patches are available in 60 x 60 m (6 x 6 pixels) and 200 x 200 m (20 x 20 pixels). Band order is B02, B03, B04, B08, B05, B06, B07, B8A, B11, B12, B01, and B09. Spatial resolution is 10 m.

    • The folder “labels” contains a JSON string which was used for multi-labeling of the training patches. Code example of an image sample with respective proportions of 94% for Abies and 6% for Larix is: "Abies_alba_3_834_WEFL_NLF.tif": [["Abies", 0.93771], ["Larix", 0.06229]]

    • The two files “test_filesnames.lst” and “train_filenames.lst” define the filenames used for train (90%) and test (10%) split. We refer to this fixed split for better reproducibility and comparability.

    • The folder “geojson” contains geoJSON files with all the samples chosen for the derivation of training patch generation (point, 60 m bounding box, 200 m bounding box).

    CAUTION: As we could not upload the aerial patches as a single zip file on Zenodo, you need to download the 20 single species files (aerial_60m_…zip) separately. Then, unzip them into a folder named “aerial” with a subfolder named “60m”. This structure is recommended for better reproducibility and comparability to the experimental results of Ahlswede et al. (2022),

    Join the archive

    Model training, benchmarking, algorithm development… many applications are possible! Feel free to add samples from other regions in Europe or even worldwide. Additional remote sensing data from Lidar, UAVs or aerial imagery from different time steps are very welcome. This helps the research community in development of better deep learning and machine learning models for forest applications. You might have questions or want to share code/results/publications using that archive? Feel free to contact the authors.

    Project description

    This work was part of the project TreeSatAI (Artificial Intelligence with Satellite data and Multi-Source Geodata for Monitoring of Trees at Infrastructures, Nature Conservation Sites and Forests). Its overall aim is the development of AI methods for the monitoring of forests and woody features on a local, regional and global scale. Based on freely available geodata from different sources (e.g., remote sensing, administration maps, and social media), prototypes will be developed for the deep learning-based extraction and classification of tree- and tree stand features. These prototypes deal with real cases from the monitoring of managed forests, nature conservation and infrastructures. The development of the resulting services by three enterprises (liveEO, Vision Impulse and LUP Potsdam) will be supported by three research institutes (German Research Center for Artificial Intelligence, TU Remote Sensing Image Analysis Group, TUB Geoinformation in Environmental Planning Lab).

    Publications

    Ahlswede et al. (2022, in prep.): TreeSatAI Dataset Publication

    Ahlswede S., Nimisha, T.M., and Demir, B. (2022, in revision): Embedded Self-Enhancement Maps for Weakly Supervised Tree Species Mapping in Remote Sensing Images. IEEE Trans Geosci Remote Sens

    Schulz et al. (2022, in prep.): Phenoprofiling

    Conference contributions

    S. Ahlswede, N. T. Madam, C. Schulz, B. Kleinschmit and B. Demіr, "Weakly Supervised Semantic Segmentation of Remote Sensing Images for Tree Species Classification Based on Explanation Methods", IEEE International Geoscience and Remote Sensing Symposium, Kuala Lumpur, Malaysia, 2022.

    C. Schulz, M. Förster, S. Vulova, T. Gränzig and B. Kleinschmit, “Exploring the temporal fingerprints of mid-European forest types from Sentinel-1 RVI and Sentinel-2 NDVI time series”, IEEE International Geoscience and Remote Sensing Symposium, Kuala Lumpur, Malaysia, 2022.

    C. Schulz, M. Förster, S. Vulova and B. Kleinschmit, “The temporal fingerprints of common European forest types from SAR and optical remote sensing data”, AGU Fall Meeting, New Orleans, USA, 2021.

    B. Kleinschmit, M. Förster, C. Schulz, F. Arias, B. Demir, S. Ahlswede, A. K. Aksoy, T. Ha Minh, J. Hees, C. Gava, P. Helber, B. Bischke, P. Habelitz, A. Frick, R. Klinke, S. Gey, D. Seidel, S. Przywarra, R. Zondag and B. Odermatt, “Artificial Intelligence with Satellite data and Multi-Source Geodata for Monitoring of Trees and Forests”, Living Planet Symposium, Bonn, Germany, 2022.

    C. Schulz, M. Förster, S. Vulova, T. Gränzig and B. Kleinschmit, (2022, submitted): “Exploring the temporal fingerprints of sixteen mid-European forest types from Sentinel-1 and Sentinel-2 time series”, ForestSAT, Berlin, Germany, 2022.

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Agricultural Research Service (2024). X-ray CT data with semantic annotations for the paper "A workflow for segmenting soil and plant X-ray CT images with deep learning in Google’s Colaboratory" [Dataset]. https://catalog.data.gov/dataset/x-ray-ct-data-with-semantic-annotations-for-the-paper-a-workflow-for-segmenting-soil-and-p-d195a
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Data from: X-ray CT data with semantic annotations for the paper "A workflow for segmenting soil and plant X-ray CT images with deep learning in Google’s Colaboratory"

Related Article
Explore at:
Dataset updated
May 2, 2024
Dataset provided by
Agricultural Research Servicehttps://www.ars.usda.gov/
Description

Leaves from genetically unique Juglans regia plants were scanned using X-ray micro-computed tomography (microCT) on the X-ray μCT beamline (8.3.2) at the Advanced Light Source (ALS) in Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA USA). Soil samples were collected in Fall of 2017 from the riparian oak forest located at the Russell Ranch Sustainable Agricultural Institute at the University of California Davis. The soil was sieved through a 2 mm mesh and was air dried before imaging. A single soil aggregate was scanned at 23 keV using the 10x objective lens with a pixel resolution of 650 nanometers on beamline 8.3.2 at the ALS. Additionally, a drought stressed almond flower bud (Prunus dulcis) from a plant housed at the University of California, Davis, was scanned using a 4x lens with a pixel resolution of 1.72 µm on beamline 8.3.2 at the ALS Raw tomographic image data was reconstructed using TomoPy. Reconstructions were converted to 8-bit tif or png format using ImageJ or the PIL package in Python before further processing. Images were annotated using Intel’s Computer Vision Annotation Tool (CVAT) and ImageJ. Both CVAT and ImageJ are free to use and open source. Leaf images were annotated in following Théroux-Rancourt et al. (2020). Specifically, Hand labeling was done directly in ImageJ by drawing around each tissue; with 5 images annotated per leaf. Care was taken to cover a range of anatomical variation to help improve the generalizability of the models to other leaves. All slices were labeled by Dr. Mina Momayyezi and Fiona Duong.To annotate the flower bud and soil aggregate, images were imported into CVAT. The exterior border of the bud (i.e. bud scales) and flower were annotated in CVAT and exported as masks. Similarly, the exterior of the soil aggregate and particulate organic matter identified by eye were annotated in CVAT and exported as masks. To annotate air spaces in both the bud and soil aggregate, images were imported into ImageJ. A gaussian blur was applied to the image to decrease noise and then the air space was segmented using thresholding. After applying the threshold, the selected air space region was converted to a binary image with white representing the air space and black representing everything else. This binary image was overlaid upon the original image and the air space within the flower bud and aggregate was selected using the “free hand” tool. Air space outside of the region of interest for both image sets was eliminated. The quality of the air space annotation was then visually inspected for accuracy against the underlying original image; incomplete annotations were corrected using the brush or pencil tool to paint missing air space white and incorrectly identified air space black. Once the annotation was satisfactorily corrected, the binary image of the air space was saved. Finally, the annotations of the bud and flower or aggregate and organic matter were opened in ImageJ and the associated air space mask was overlaid on top of them forming a three-layer mask suitable for training the fully convolutional network. All labeling of the soil aggregate and soil aggregate images was done by Dr. Devin Rippner. These images and annotations are for training deep learning models to identify different constituents in leaves, almond buds, and soil aggregates Limitations: For the walnut leaves, some tissues (stomata, etc.) are not labeled and only represent a small portion of a full leaf. Similarly, both the almond bud and the aggregate represent just one single sample of each. The bud tissues are only divided up into buds scales, flower, and air space. Many other tissues remain unlabeled. For the soil aggregate annotated labels are done by eye with no actual chemical information. Therefore particulate organic matter identification may be incorrect. Resources in this dataset:Resource Title: Annotated X-ray CT images and masks of a Forest Soil Aggregate. File Name: forest_soil_images_masks_for_testing_training.zipResource Description: This aggregate was collected from the riparian oak forest at the Russell Ranch Sustainable Agricultural Facility. The aggreagate was scanned using X-ray micro-computed tomography (microCT) on the X-ray μCT beamline (8.3.2) at the Advanced Light Source (ALS) in Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA USA) using the 10x objective lens with a pixel resolution of 650 nanometers. For masks, the background has a value of 0,0,0; pores spaces have a value of 250,250, 250; mineral solids have a value= 128,0,0; and particulate organic matter has a value of = 000,128,000. These files were used for training a model to segment the forest soil aggregate and for testing the accuracy, precision, recall, and f1 score of the model.Resource Title: Annotated X-ray CT images and masks of an Almond bud (P. Dulcis). File Name: Almond_bud_tube_D_P6_training_testing_images_and_masks.zipResource Description: Drought stressed almond flower bud (Prunis dulcis) from a plant housed at the University of California, Davis, was scanned by X-ray micro-computed tomography (microCT) on the X-ray μCT beamline (8.3.2) at the Advanced Light Source (ALS) in Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA USA) using the 4x lens with a pixel resolution of 1.72 µm using. For masks, the background has a value of 0,0,0; air spaces have a value of 255,255, 255; bud scales have a value= 128,0,0; and flower tissues have a value of = 000,128,000. These files were used for training a model to segment the almond bud and for testing the accuracy, precision, recall, and f1 score of the model.Resource Software Recommended: Fiji (ImageJ),url: https://imagej.net/software/fiji/downloads Resource Title: Annotated X-ray CT images and masks of Walnut leaves (J. Regia) . File Name: 6_leaf_training_testing_images_and_masks_for_paper.zipResource Description: Stems were collected from genetically unique J. regia accessions at the 117 USDA-ARS-NCGR in Wolfskill Experimental Orchard, Winters, California USA to use as scion, and were grafted by Sierra Gold Nursery onto a commonly used commercial rootstock, RX1 (J. microcarpa × J. regia). We used a common rootstock to eliminate any own-root effects and to simulate conditions for a commercial walnut orchard setting, where rootstocks are commonly used. The grafted saplings were repotted and transferred to the Armstrong lathe house facility at the University of California, Davis in June 2019, and kept under natural light and temperature. Leaves from each accession and treatment were scanned using X-ray micro-computed tomography (microCT) on the X-ray μCT beamline (8.3.2) at the Advanced Light Source (ALS) in Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA USA) using the 10x objective lens with a pixel resolution of 650 nanometers. For masks, the background has a value of 170,170,170; Epidermis value= 85,85,85; Mesophyll value= 0,0,0; Bundle Sheath Extension value= 152,152,152; Vein value= 220,220,220; Air value = 255,255,255.Resource Software Recommended: Fiji (ImageJ),url: https://imagej.net/software/fiji/downloads

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