Government Facilities in Frederick County, Maryland

Detailed information about the Organisation ORG-GIS2-RIPE.

GIS2.GIS.fibertext

FunCoup network information for gene GIS2 in Arabidopsis thaliana. GIS2_ARATH Zinc finger protein GIS2

Protein-Protein, Genetic, and Chemical Interactions for Sammons MA (2011):Saccharomyces cerevisiae Gis2 interacts with the translation machinery and is orthogonal to myotonic dystrophy type 2 protein ZNF9. curated by BioGRID (https://thebiogrid.org); ABSTRACT: The myotonic dystrophy type 2 protein ZNF9/CNBP is a small nucleic acid binding protein proposed to act as a regulator of transcription and translation. The precise functions and activity of this protein are poorly understood. Previous studies suggested that ZNF9 regulates translation and facilitates the process of cap-independent translation through interactions with mRNA and the translating ribosome. To help determine the role played by ZNF9 in the activation translation initiation, we combined genetic and biochemical analysis of the putative ZNF9 ortholog GIS2, in the budding yeast Saccharomyces cerevisiae. Purification of the Gis2p protein followed by mass spectrometry based-proteomic analysis identified a large number of co-purifying ribosomal subunits and translation factors, strongly suggesting that Gis2p interacts with the protein translation machinery. Polysome profiling and ribosome isolation experiments confirm that Gis2p physically interacts with the translating ribosome. Interestingly, expression of yeast Gis2p in HEK293T cells activates cap-independent translation driven by the 5'UTR of the ODC gene. These data suggest that Gis2 is functionally orthologous to ZNF9 and acts as a cap-independent translation factor.

This entry represents a group of C2H2 transcription factors, including GIS, ZFP8 and GIS2 from Arabidopsis. They play partially redundant and essential roles in inflorescence trichome initiation and in its regulation by GA and cytokinins .

GIS2_YEAST Zinc finger protein GIS2

Protein-Protein, Genetic, and Chemical Interactions for GIS2 (Arabidopsis thaliana (Columbia)) curated by BioGRID (https://thebiogrid.org); DEFINITION: protein GLABROUS INFLORESCENCE STEMS 2

Protein-Protein, Genetic, and Chemical Interactions for GIS2 (Saccharomyces cerevisiae (S288c)) curated by BioGRID (https://thebiogrid.org); DEFINITION: Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2

2D island map GIS files

The yeast protein PBP1 is implicated in very diverse pathways. Intriguingly, its deletion mitigates the toxicity of human neurodegeneration factors. Here, we performed label-free quantitative global proteomics to identify crucial downstream factors, either without stress or under cell stress conditions (heat and NaN3). Compared to the wildtype BY4741 strain, PBP1 deletion always triggered downregulation of the key bioenergetics enzyme KGD2 and the prion protein RNQ1 as well as upregulation of the leucine biosynthesis enzyme LEU1. Without stress, enrichment of stress response factors was consistently detected for both deletion mutants; upon stress, these factors were more pronounced. The selective analysis of components of stress granules and P-bodies revealed a prominent downregulation of GIS2. Our yeast data are in good agreement with a global proteomics and metabolomics publication that the PBP1 ortholog ATAXIN-2 (ATXN2) knockout (KO) in mouse results in mitochondrial deficits in leucine/fatty acid catabolism and bioenergetics, with an obesity phenotype. Furthermore, our data provide the completely novel insight that PBP1 mutations in stress periods involve GIS2, a plausible scenario in view of previous data that both PBP1 and GIS2 relocalize from ribosomes to stress granules, interact with poly(A)-binding protein in translation regulation and prevent mitochondrial precursor overaccumulation stress (mPOS). This may be relevant for human diseases like spinocerebellar ataxias, amyotrophic lateral sclerosis, and the metabolic syndrome.

This dataset contains forest features in Frederick County. Existng forest outlines that have been changed by cutting, burning, or other means of removal were reflected in the deliverable. Any new forest features were captured at the outer edges of the tree trunks. Forest is defined as the edge of a tree mass of 10,000 square feet or greater, not less than 35' in length and follows along the outer edge of the tree trunks.

OLS_Regression

EarthAdvantage_Data_Geocoded

Validation of results for 2D and 3D noise mapping.

Discover the worldwide distribution of the Gis surname. Present in 82 countries with 5,370 registered people.

GetisG_HotSpots_Avg_HES

Noise monitoring location, date and time.

Description of noise monitoring stations.

The Comprehensive Land Use Plan is a subset of various layers, which are intended to represent how Frederick County may develop within the next 20 - 30 years and is sanctioned by the Frederick County Board of County Commissioners. These data sets, which include the Comprehensive Land Use Plan Designations, Community Facilities, Highway Plan, and Community Growth Boundaries, should be used together in order to fully represent and understand how Frederick County may grow within the next 20-30 years. Using the individual layers alone may misrepresent the Comprehensive Land Use Plan. The most recent Countywide Comprehensive Plan was adopted on April 8, 2010. This data is not a reflection of current ground conditions nor should it be used for engineering or surveying purposes. The Community Growth Boundaries indicate areas where residential and commercial development should take place. The data was adopted as part of the April 2010 Countywide Comprehensive Plan. The Areas were amended September 13, 2012. This data should be used in conjunction with the other Comprehensive Land Use Plan layers in order to fully understand and properly represent the Comprehensive Land Use Plan. This data is for display purposes only.