Despite animal testing being a controversial topic for many years, it is still widely used globally to assess the safety of products and test the efficacy of new treatments and products. In 2020, the United States was the world’s largest user of animals in research and testing, with around 20 million animals used research and testing, followed by China where it is estimated that around 16 million animals were used in research and testing in that year. Animal testing is used especially in the medical, cosmetic, and chemical industries.

Animal Testing in the EU

The European Union also reported some 9.4 million animals used research and testing as of 2020. Basic research, and translational and applied research are the two leading purposes of animal testing in the European Union. Mice represent the most commonly used animal in research and testing in the EU, representing almost half of all animals used in research and testing, followed by fish and rats.

Animal Testing in Great Britain

Animal testing in Great Britain was most common in basic scientific research on the nervous system and the immune system, and most procedures on animals for scientific experiments in that year in Great Britain were conducted by universities and medical schools. As in the EU, mice were the most commonly used animals in research and testing, followed by domestic fowl and rats.

In 2019, 797,546 animals were used for research in research facilities in the United States. This is an increase from the previous year, when about 780,070 animals were used for research in the country.

This statistic displays the total number of animals used in scientific research in the European Union from 2015 to 2022, including only animals used for the first time each year. In 2022, some 8.4 million animals were used in scientific research in all EU member states and Norway, an increase of over 400 thousand compared to the previous year.

This statistic shows the likelihood of consumers in the United States to stop purchasing from their favorite cosmetics/makeup brand if it were reported that the brand test their products on animals, as of April 2017. During the survey, 32 percent of consumers reported that they would very likely stop purchasing from their favorite brand if they tested on animals.

Global Animals Used in Research and Testing market size 2025 was XX Million. Animals Used in Research and Testing Industry compound annual growth rate (CAGR) will be XX% from 2025 till 2033.

This statistic displays the share of purposes for scientific research on animals in the 27 European Union countries (including Norway) in 2019. The main purpose for testing on animals was for basic research, with some 45 percent.

This statistic displays the number of animals used in scientific research in Ireland in 2023. Mice were the most commonly used animal, accounting for nearly 87 thousand uses, followed by rats at more than eight thousand.

This statistic shows how likely consumers in the United States would be to stop purchasing from their favorite cosmetics/makeup brand if it were reported that they test their products on animals, as of April 2017, by age. During the survey, ** percent of respondents aged 35 to 54 years reported that they would very likely stop purchasing from their favorite brand if they tested on animals.

This statistic shows the moral stance of Americans regarding medical testing on animals in 2018. During the survey, 54 percent of respondents stated that they think medical testing on animals is morally acceptable, while 2 percent said it depends.

Financial overview and grant giving statistics of American Fund For Alternatives To Animal Research

Financial overview and grant giving statistics of Center for Animal Research and Education Inc.

The Animal Experiment Mice market plays a critical role in biomedical research, facilitating advancements in medicine, genetics, and toxicology. Mice are frequently the preferred animal model due to their physiological and genetic similarities to humans, making them invaluable for studying diseases, testing drugs, a

Ruminant Imports Official Bluetongue Test Results IRL 2017 to 2021 . Published by Department of Agriculture, Food and the Marine. Available under the license cc-by (CC-BY-4.0).Bluetongue ELISA test results of ruminants (cattle, sheep and goats) imported into Ireland over the period Jan 2017 to Dec 2021. ELISA used detects all serotypes of Bluetongue (wild virus and current vaccines) currently found in Europe. As quarantine procedures encourage double sampling of imported animals, some results will be from the same animal tested twice. ...

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In-Vitro Toxicity Testing Market Size 2025-2029

The in-vitro toxicity testing market size is forecast to increase by USD 8.19 billion, at a CAGR of 11.7% between 2024 and 2029. Increasing use of in-vitro toxicity testing by companies to reduce drug failures will drive the in-vitro toxicity testing market.

Major Market Trends & Insights

North America dominated the market and accounted for a 39% growth during the forecast period.
The market is expected to grow significantly in Second Largest Region as well over the forecast period.
By the End-user, the Pharmaceutical companies sub-segment was valued at USD billion in 2023
By the Type, the Cytotoxicity testing sub-segment accounted for the largest market revenue share in 2023

Market Size & Forecast

Market Opportunities: USD 172.11 billion
Future Opportunities: USD 8.19 billion 
CAGR : 11.7%
North America: Largest market in 2023

Market Summary

The In-Vitro Toxicity Testing Market is expanding as a critical alternative to traditional animal testing methods. The market's growth is primarily driven by ethical concerns, stringent global regulations, and technological advancements like cellular assays, high-throughput screening, and the development of 3D cell cultures. The pharmaceutical and cosmetics sectors are major adopters, with over 60% of pharmaceutical companies now incorporating in-vitro assays into their safety assessment protocols to reduce costs and accelerate time-to-market.
These innovations enable precise testing for endpoints like genotoxicity, dermal toxicity, and ocular toxicity, which are vital for ensuring product safety. The market also focuses on areas like in-silico toxicology for predictive modeling. The dynamics of this market are mirrored in related fields such as the Drug Discovery and Development and Personalized Medicine markets, where similar pressures for cost-effective, high-precision testing methods are present.

What will be the Size of the In-Vitro Toxicity Testing Market during the forecast period?

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How is the In-Vitro Toxicity Testing Market Segmented and what are the key trends of market segmentation?

The in-vitro toxicity testing industry research report provides comprehensive data (region-wise segment analysis), with forecasts and estimates in 'USD million' for the period 2025-2029, as well as historical data from 2019-2023 for the following segments.

End-user

  Pharmaceutical companies
  Academic and research institutions
  Others


Type

  Cytotoxicity testing
  Genotoxicity testing
  Organ-specific toxicity testing
  Phototoxicity testing
  Dermal toxicity testing


Technology

  Cell culture technology
  High throughput screening
  OMICS technology
  Computational toxicology


Geography

  North America

    US
    Canada


  Europe

    France
    Germany
    Italy
    UK


  APAC

    China
    India
    Japan


  South America

    Brazil


  Rest of World (ROW)

By End-user Insights

The pharmaceutical companies segment is estimated to witness significant growth during the forecast period.

In-vitro toxicity testing plays a pivotal role in the drug development process for pharmaceutical and biotechnology companies. With the increasing complexity of the drug development process, which includes clinical trials, regulatory processes, safety studies, approval processes, and quality checks, the chances of drug rejection at later stages have increased. Historically, lack of in-vitro toxicity testing was a significant reason for drug approval failures. To mitigate these risks, companies are turning to in-vitro toxicity testing. This testing method employs various assays, such as cell cycle arrest, data analysis software, caspase activity, reactive oxygen species, LDH assay, cell proliferation, OECD guidelines, apoptosis induction, in vitro genotoxicity, chromosome aberration assay, high-content screening, EC50 determination, enzyme activity, ISO standards, MTT assay, gene expression, automated microscopy, oxidative stress, GLP compliance, inflammation markers, DNA damage, cytotoxicity testing, cell viability assay, necrosis detection, protein expression, Ames test, toxicity prediction models, IC50 determination, comet assay, and image analysis.

These tests help assess the potential toxic effects of drugs on various cell types and predict their safety profiles. For instance, cell cycle arrest assays determine the ability of a substance to halt the progression of the cell cycle, while caspase activity assays measure apoptosis induction. Reactive oxygen species and LDH assays assess oxidative stress and cell damage, respectively. The importance of in-vitro toxicity testing is underscored by the following statistics. Currently, approximately 35% of drugs entering clinical trials fail due to safety concerns. Moreover, about 40% of dru