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Replication pack, FSE2018 submission #164: ------------------------------------------
**Working title:** Ecosystem-Level Factors Affecting the Survival of Open-Source Projects: A Case Study of the PyPI Ecosystem **Note:** link to data artifacts is already included in the paper. Link to the code will be included in the Camera Ready version as well. Content description =================== - **ghd-0.1.0.zip** - the code archive. This code produces the dataset files described below - **settings.py** - settings template for the code archive. - **dataset_minimal_Jan_2018.zip** - the minimally sufficient version of the dataset. This dataset only includes stats aggregated by the ecosystem (PyPI) - **dataset_full_Jan_2018.tgz** - full version of the dataset, including project-level statistics. It is ~34Gb unpacked. This dataset still doesn't include PyPI packages themselves, which take around 2TB. - **build_model.r, helpers.r** - R files to process the survival data (`survival_data.csv` in **dataset_minimal_Jan_2018.zip**, `common.cache/survival_data.pypi_2008_2017-12_6.csv` in **dataset_full_Jan_2018.tgz**) - **Interview protocol.pdf** - approximate protocol used for semistructured interviews. - LICENSE - text of GPL v3, under which this dataset is published - INSTALL.md - replication guide (~2 pages)
Replication guide ================= Step 0 - prerequisites ---------------------- - Unix-compatible OS (Linux or OS X) - Python interpreter (2.7 was used; Python 3 compatibility is highly likely) - R 3.4 or higher (3.4.4 was used, 3.2 is known to be incompatible) Depending on detalization level (see Step 2 for more details): - up to 2Tb of disk space (see Step 2 detalization levels) - at least 16Gb of RAM (64 preferable) - few hours to few month of processing time Step 1 - software ---------------- - unpack **ghd-0.1.0.zip**, or clone from gitlab: git clone https://gitlab.com/user2589/ghd.git git checkout 0.1.0 `cd` into the extracted folder. All commands below assume it as a current directory. - copy `settings.py` into the extracted folder. Edit the file: * set `DATASET_PATH` to some newly created folder path * add at least one GitHub API token to `SCRAPER_GITHUB_API_TOKENS` - install docker. For Ubuntu Linux, the command is `sudo apt-get install docker-compose` - install libarchive and headers: `sudo apt-get install libarchive-dev` - (optional) to replicate on NPM, install yajl: `sudo apt-get install yajl-tools` Without this dependency, you might get an error on the next step, but it's safe to ignore. - install Python libraries: `pip install --user -r requirements.txt` . - disable all APIs except GitHub (Bitbucket and Gitlab support were not yet implemented when this study was in progress): edit `scraper/init.py`, comment out everything except GitHub support in `PROVIDERS`. Step 2 - obtaining the dataset ----------------------------- The ultimate goal of this step is to get output of the Python function `common.utils.survival_data()` and save it into a CSV file: # copy and paste into a Python console from common import utils survival_data = utils.survival_data('pypi', '2008', smoothing=6) survival_data.to_csv('survival_data.csv') Since full replication will take several months, here are some ways to speedup the process: ####Option 2.a, difficulty level: easiest Just use the precomputed data. Step 1 is not necessary under this scenario. - extract **dataset_minimal_Jan_2018.zip** - get `survival_data.csv`, go to the next step ####Option 2.b, difficulty level: easy Use precomputed longitudinal feature values to build the final table. The whole process will take 15..30 minutes. - create a folder `
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This dataset includes all the data and R code needed to reproduce the analyses in a forthcoming manuscript:Copes, W. E., Q. D. Read, and B. J. Smith. Environmental influences on drying rate of spray applied disinfestants from horticultural production services. PhytoFrontiers, DOI pending.Study description: Instructions for disinfestants typically specify a dose and a contact time to kill plant pathogens on production surfaces. A problem occurs when disinfestants are applied to large production areas where the evaporation rate is affected by weather conditions. The common contact time recommendation of 10 min may not be achieved under hot, sunny conditions that promote fast drying. This study is an investigation into how the evaporation rates of six commercial disinfestants vary when applied to six types of substrate materials under cool to hot and cloudy to sunny weather conditions. Initially, disinfestants with low surface tension spread out to provide 100% coverage and disinfestants with high surface tension beaded up to provide about 60% coverage when applied to hard smooth surfaces. Disinfestants applied to porous materials were quickly absorbed into the body of the material, such as wood and concrete. Even though disinfestants evaporated faster under hot sunny conditions than under cool cloudy conditions, coverage was reduced considerably in the first 2.5 min under most weather conditions and reduced to less than or equal to 50% coverage by 5 min. Dataset contents: This dataset includes R code to import the data and fit Bayesian statistical models using the model fitting software CmdStan, interfaced with R using the packages brms and cmdstanr. The models (one for 2022 and one for 2023) compare how quickly different spray-applied disinfestants dry, depending on what chemical was sprayed, what surface material it was sprayed onto, and what the weather conditions were at the time. Next, the statistical models are used to generate predictions and compare mean drying rates between the disinfestants, surface materials, and weather conditions. Finally, tables and figures are created. These files are included:Drying2022.csv: drying rate data for the 2022 experimental runWeather2022.csv: weather data for the 2022 experimental runDrying2023.csv: drying rate data for the 2023 experimental runWeather2023.csv: weather data for the 2023 experimental rundisinfestant_drying_analysis.Rmd: RMarkdown notebook with all data processing, analysis, and table creation codedisinfestant_drying_analysis.html: rendered output of notebookMS_figures.R: additional R code to create figures formatted for journal requirementsfit2022_discretetime_weather_solar.rds: fitted brms model object for 2022. This will allow users to reproduce the model prediction results without having to refit the model, which was originally fit on a high-performance computing clusterfit2023_discretetime_weather_solar.rds: fitted brms model object for 2023data_dictionary.xlsx: descriptions of each column in the CSV data files
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TwitterTo make this a seamless process, I cleaned the data and delete many variables that I thought were not important to our dataset. I then uploaded all of those files to Kaggle for each of you to download. The rideshare_data has both lyft and uber but it is still a cleaned version from the dataset we downloaded from Kaggle.
You can easily subset the data into the car types that you will be modeling by first loading the csv into R, here is the code for how you do this:
df<-read.csv('uber.csv')
df_black<-subset(uber_df, uber_df$name == 'Black')
write.csv(df_black, "nameofthefileyouwanttosaveas.csv")
getwd()
Your data will be in front of the world's largest data science community. What questions do you want to see answered?
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TwitterThis archive contains code and data for reproducing the analysis for “Replication Data for Revisiting ‘The Rise and Decline’ in a Population of Peer Production Projects”. Depending on what you hope to do with the data you probabbly do not want to download all of the files. Depending on your computation resources you may not be able to run all stages of the analysis. The code for all stages of the analysis, including typesetting the manuscript and running the analysis, is in code.tar. If you only want to run the final analysis or to play with datasets used in the analysis of the paper, you want intermediate_data.7z or the uncompressed tab and csv files. The data files are created in a four-stage process. The first stage uses the program “wikiq” to parse mediawiki xml dumps and create tsv files that have edit data for each wiki. The second stage generates all.edits.RDS file which combines these tsvs into a dataset of edits from all the wikis. This file is expensive to generate and at 1.5GB is pretty big. The third stage builds smaller intermediate files that contain the analytical variables from these tsv files. The fourth stage uses the intermediate files to generate smaller RDS files that contain the results. Finally, knitr and latex typeset the manuscript. A stage will only run if the outputs from the previous stages do not exist. So if the intermediate files exist they will not be regenerated. Only the final analysis will run. The exception is that stage 4, fitting models and generating plots, always runs. If you only want to replicate from the second stage onward, you want wikiq_tsvs.7z. If you want to replicate everything, you want wikia_mediawiki_xml_dumps.7z.001 wikia_mediawiki_xml_dumps.7z.002, and wikia_mediawiki_xml_dumps.7z.003. These instructions work backwards from building the manuscript using knitr, loading the datasets, running the analysis, to building the intermediate datasets. Building the manuscript using knitr This requires working latex, latexmk, and knitr installations. Depending on your operating system you might install these packages in different ways. On Debian Linux you can run apt install r-cran-knitr latexmk texlive-latex-extra. Alternatively, you can upload the necessary files to a project on Overleaf.com. Download code.tar. This has everything you need to typeset the manuscript. Unpack the tar archive. On a unix system this can be done by running tar xf code.tar. Navigate to code/paper_source. Install R dependencies. In R. run install.packages(c("data.table","scales","ggplot2","lubridate","texreg")) On a unix system you should be able to run make to build the manuscript generalizable_wiki.pdf. Otherwise you should try uploading all of the files (including the tables, figure, and knitr folders) to a new project on Overleaf.com. Loading intermediate datasets The intermediate datasets are found in the intermediate_data.7z archive. They can be extracted on a unix system using the command 7z x intermediate_data.7z. The files are 95MB uncompressed. These are RDS (R data set) files and can be loaded in R using the readRDS. For example newcomer.ds <- readRDS("newcomers.RDS"). If you wish to work with these datasets using a tool other than R, you might prefer to work with the .tab files. Running the analysis Fitting the models may not work on machines with less than 32GB of RAM. If you have trouble, you may find the functions in lib-01-sample-datasets.R useful to create stratified samples of data for fitting models. See line 89 of 02_model_newcomer_survival.R for an example. Download code.tar and intermediate_data.7z to your working folder and extract both archives. On a unix system this can be done with the command tar xf code.tar && 7z x intermediate_data.7z. Install R dependencies. install.packages(c("data.table","ggplot2","urltools","texreg","optimx","lme4","bootstrap","scales","effects","lubridate","devtools","roxygen2")). On a unix system you can simply run regen.all.sh to fit the models, build the plots and create the RDS files. Generating datasets Building the intermediate files The intermediate files are generated from all.edits.RDS. This process requires about 20GB of memory. Download all.edits.RDS, userroles_data.7z,selected.wikis.csv, and code.tar. Unpack code.tar and userroles_data.7z. On a unix system this can be done using tar xf code.tar && 7z x userroles_data.7z. Install R dependencies. In R run install.packages(c("data.table","ggplot2","urltools","texreg","optimx","lme4","bootstrap","scales","effects","lubridate","devtools","roxygen2")). Run 01_build_datasets.R. Building all.edits.RDS The intermediate RDS files used in the analysis are created from all.edits.RDS. To replicate building all.edits.RDS, you only need to run 01_build_datasets.R when the int... Visit https://dataone.org/datasets/sha256%3Acfa4980c107154267d8eb6dc0753ed0fde655a73a062c0c2f5af33f237da3437 for complete metadata about this dataset.
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Categorical scatterplots with R for biologists: a step-by-step guide
Benjamin Petre1, Aurore Coince2, Sophien Kamoun1
1 The Sainsbury Laboratory, Norwich, UK; 2 Earlham Institute, Norwich, UK
Weissgerber and colleagues (2015) recently stated that ‘as scientists, we urgently need to change our practices for presenting continuous data in small sample size studies’. They called for more scatterplot and boxplot representations in scientific papers, which ‘allow readers to critically evaluate continuous data’ (Weissgerber et al., 2015). In the Kamoun Lab at The Sainsbury Laboratory, we recently implemented a protocol to generate categorical scatterplots (Petre et al., 2016; Dagdas et al., 2016). Here we describe the three steps of this protocol: 1) formatting of the data set in a .csv file, 2) execution of the R script to generate the graph, and 3) export of the graph as a .pdf file.
Protocol
• Step 1: format the data set as a .csv file. Store the data in a three-column excel file as shown in Powerpoint slide. The first column ‘Replicate’ indicates the biological replicates. In the example, the month and year during which the replicate was performed is indicated. The second column ‘Condition’ indicates the conditions of the experiment (in the example, a wild type and two mutants called A and B). The third column ‘Value’ contains continuous values. Save the Excel file as a .csv file (File -> Save as -> in ‘File Format’, select .csv). This .csv file is the input file to import in R.
• Step 2: execute the R script (see Notes 1 and 2). Copy the script shown in Powerpoint slide and paste it in the R console. Execute the script. In the dialog box, select the input .csv file from step 1. The categorical scatterplot will appear in a separate window. Dots represent the values for each sample; colors indicate replicates. Boxplots are superimposed; black dots indicate outliers.
• Step 3: save the graph as a .pdf file. Shape the window at your convenience and save the graph as a .pdf file (File -> Save as). See Powerpoint slide for an example.
Notes
• Note 1: install the ggplot2 package. The R script requires the package ‘ggplot2’ to be installed. To install it, Packages & Data -> Package Installer -> enter ‘ggplot2’ in the Package Search space and click on ‘Get List’. Select ‘ggplot2’ in the Package column and click on ‘Install Selected’. Install all dependencies as well.
• Note 2: use a log scale for the y-axis. To use a log scale for the y-axis of the graph, use the command line below in place of command line #7 in the script.
replicates
graph + geom_boxplot(outlier.colour='black', colour='black') + geom_jitter(aes(col=Replicate)) + scale_y_log10() + theme_bw()
References
Dagdas YF, Belhaj K, Maqbool A, Chaparro-Garcia A, Pandey P, Petre B, et al. (2016) An effector of the Irish potato famine pathogen antagonizes a host autophagy cargo receptor. eLife 5:e10856.
Petre B, Saunders DGO, Sklenar J, Lorrain C, Krasileva KV, Win J, et al. (2016) Heterologous Expression Screens in Nicotiana benthamiana Identify a Candidate Effector of the Wheat Yellow Rust Pathogen that Associates with Processing Bodies. PLoS ONE 11(2):e0149035
Weissgerber TL, Milic NM, Winham SJ, Garovic VD (2015) Beyond Bar and Line Graphs: Time for a New Data Presentation Paradigm. PLoS Biol 13(4):e1002128
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Pathogen diversity resulting in quasispecies can enable persistence and adaptation to host defenses and therapies. However, accurate quasispecies characterization can be impeded by errors introduced during sample handling and sequencing which can require extensive optimizations to overcome. We present complete laboratory and bioinformatics workflows to overcome many of these hurdles. The Pacific Biosciences single molecule real-time platform was used to sequence PCR amplicons derived from cDNA templates tagged with universal molecular identifiers (SMRT-UMI). Optimized laboratory protocols were developed through extensive testing of different sample preparation conditions to minimize between-template recombination during PCR and the use of UMI allowed accurate template quantitation as well as removal of point mutations introduced during PCR and sequencing to produce a highly accurate consensus sequence from each template. Handling of the large datasets produced from SMRT-UMI sequencing was facilitated by a novel bioinformatic pipeline, Probabilistic Offspring Resolver for Primer IDs (PORPIDpipeline), that automatically filters and parses reads by sample, identifies and discards reads with UMIs likely created from PCR and sequencing errors, generates consensus sequences, checks for contamination within the dataset, and removes any sequence with evidence of PCR recombination or early cycle PCR errors, resulting in highly accurate sequence datasets. The optimized SMRT-UMI sequencing method presented here represents a highly adaptable and established starting point for accurate sequencing of diverse pathogens. These methods are illustrated through characterization of human immunodeficiency virus (HIV) quasispecies.
Methods
This serves as an overview of the analysis performed on PacBio sequence data that is summarized in Analysis Flowchart.pdf and was used as primary data for the paper by Westfall et al. "Optimized SMRT-UMI protocol produces highly accurate sequence datasets from diverse populations – application to HIV-1 quasispecies"
Five different PacBio sequencing datasets were used for this analysis: M027, M2199, M1567, M004, and M005
For the datasets which were indexed (M027, M2199), CCS reads from PacBio sequencing files and the chunked_demux_config files were used as input for the chunked_demux pipeline. Each config file lists the different Index primers added during PCR to each sample. The pipeline produces one fastq file for each Index primer combination in the config. For example, in dataset M027 there were 3–4 samples using each Index combination. The fastq files from each demultiplexed read set were moved to the sUMI_dUMI_comparison pipeline fastq folder for further demultiplexing by sample and consensus generation with that pipeline. More information about the chunked_demux pipeline can be found in the README.md file on GitHub.
The demultiplexed read collections from the chunked_demux pipeline or CCS read files from datasets which were not indexed (M1567, M004, M005) were each used as input for the sUMI_dUMI_comparison pipeline along with each dataset's config file. Each config file contains the primer sequences for each sample (including the sample ID block in the cDNA primer) and further demultiplexes the reads to prepare data tables summarizing all of the UMI sequences and counts for each family (tagged.tar.gz) as well as consensus sequences from each sUMI and rank 1 dUMI family (consensus.tar.gz). More information about the sUMI_dUMI_comparison pipeline can be found in the paper and the README.md file on GitHub.
The consensus.tar.gz and tagged.tar.gz files were moved from sUMI_dUMI_comparison pipeline directory on the server to the Pipeline_Outputs folder in this analysis directory for each dataset and appended with the dataset name (e.g. consensus_M027.tar.gz). Also in this analysis directory is a Sample_Info_Table.csv containing information about how each of the samples was prepared, such as purification methods and number of PCRs. There are also three other folders: Sequence_Analysis, Indentifying_Recombinant_Reads, and Figures. Each has an .Rmd file with the same name inside which is used to collect, summarize, and analyze the data. All of these collections of code were written and executed in RStudio to track notes and summarize results.
Sequence_Analysis.Rmd has instructions to decompress all of the consensus.tar.gz files, combine them, and create two fasta files, one with all sUMI and one with all dUMI sequences. Using these as input, two data tables were created, that summarize all sequences and read counts for each sample that pass various criteria. These are used to help create Table 2 and as input for Indentifying_Recombinant_Reads.Rmd and Figures.Rmd. Next, 2 fasta files containing all of the rank 1 dUMI sequences and the matching sUMI sequences were created. These were used as input for the python script compare_seqs.py which identifies any matched sequences that are different between sUMI and dUMI read collections. This information was also used to help create Table 2. Finally, to populate the table with the number of sequences and bases in each sequence subset of interest, different sequence collections were saved and viewed in the Geneious program.
To investigate the cause of sequences where the sUMI and dUMI sequences do not match, tagged.tar.gz was decompressed and for each family with discordant sUMI and dUMI sequences the reads from the UMI1_keeping directory were aligned using geneious. Reads from dUMI families failing the 0.7 filter were also aligned in Genious. The uncompressed tagged folder was then removed to save space. These read collections contain all of the reads in a UMI1 family and still include the UMI2 sequence. By examining the alignment and specifically the UMI2 sequences, the site of the discordance and its case were identified for each family as described in the paper. These alignments were saved as "Sequence Alignments.geneious". The counts of how many families were the result of PCR recombination were used in the body of the paper.
Using Identifying_Recombinant_Reads.Rmd, the dUMI_ranked.csv file from each sample was extracted from all of the tagged.tar.gz files, combined and used as input to create a single dataset containing all UMI information from all samples. This file dUMI_df.csv was used as input for Figures.Rmd.
Figures.Rmd used dUMI_df.csv, sequence_counts.csv, and read_counts.csv as input to create draft figures and then individual datasets for eachFigure. These were copied into Prism software to create the final figures for the paper.
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This dataset provides grayscale pixel values for brain tumor MRI images, stored in a CSV format for simplified access and ease of use. The goal is to create a "MNIST-like" dataset for brain tumors, where each row in the CSV file represents the pixel values of a single image in its original resolution. This format makes it convenient for researchers and developers to quickly load and analyze MRI data for brain tumor detection, classification, and segmentation tasks without needing to handle large image files directly.
Brain tumor classification and segmentation are critical tasks in medical imaging, and datasets like these are valuable for developing and testing machine learning and deep learning models. While there are several publicly available brain tumor image datasets, they often consist of large image files that can be challenging to process. This CSV-based dataset addresses that by providing a compact and accessible format. Potential use cases include: - Tumor Classification: Identifying different types of brain tumors, such as glioma, meningioma, and pituitary tumors, or distinguishing between tumor and non-tumor images. - Tumor Segmentation: Applying pixel-level classification and segmentation techniques for tumor boundary detection. - Educational and Rapid Prototyping: Ideal for educational purposes or quick experimentation without requiring large image processing capabilities.
This dataset is structured as a single CSV file where each row represents an image, and each column represents a grayscale pixel value. The pixel values are stored as integers ranging from 0 (black) to 255 (white).
This dataset is intended for research and educational purposes only. Users are encouraged to cite and credit the original data sources if using this dataset in any publications or projects. This is a derived CSV version aimed to simplify access and usability for machine learning and data science applications.
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Database of Uniaxial Cyclic and Tensile Coupon Tests for Structural Metallic Materials
Background
This dataset contains data from monotonic and cyclic loading experiments on structural metallic materials. The materials are primarily structural steels and one iron-based shape memory alloy is also included. Summary files are included that provide an overview of the database and data from the individual experiments is also included.
The files included in the database are outlined below and the format of the files is briefly described. Additional information regarding the formatting can be found through the post-processing library (https://github.com/ahartloper/rlmtp/tree/master/protocols).
Usage
Included Files
File Format: Downsampled Data
These are the "LP_
These data files can be easily loaded using the pandas library in Python through:
import pandas
data = pandas.read_csv(data_file, index_col=0)
The data is formatted so it can be used directly in RESSPyLab (https://github.com/AlbanoCastroSousa/RESSPyLab). Note that the column names "e_true" and "Sigma_true" were kept for backwards compatibility reasons with RESSPyLab.
File Format: Unreduced Data
These are the "LP_
The data can be loaded and used similarly to the downsampled data.
File Format: Overall_Summary
The overall summary file provides data on all the test specimens in the database. The columns include:
File Format: Summarized_Mechanical_Props_Campaign
Meant to be loaded in Python as a pandas DataFrame with multi-indexing, e.g.,
tab1 = pd.read_csv('Summarized_Mechanical_Props_Campaign_' + date + version + '.csv',
index_col=[0, 1, 2, 3], skipinitialspace=True, header=[0, 1],
keep_default_na=False, na_values='')
Caveats
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Overview
This dataset is the repository for the following paper submitted to Data in Brief:
Kempf, M. A dataset to model Levantine landcover and land-use change connected to climate change, the Arab Spring and COVID-19. Data in Brief (submitted: December 2023).
The Data in Brief article contains the supplement information and is the related data paper to:
Kempf, M. Climate change, the Arab Spring, and COVID-19 - Impacts on landcover transformations in the Levant. Journal of Arid Environments (revision submitted: December 2023).
Description/abstract
The Levant region is highly vulnerable to climate change, experiencing prolonged heat waves that have led to societal crises and population displacement. Since 2010, the area has been marked by socio-political turmoil, including the Syrian civil war and currently the escalation of the so-called Israeli-Palestinian Conflict, which strained neighbouring countries like Jordan due to the influx of Syrian refugees and increases population vulnerability to governmental decision-making. Jordan, in particular, has seen rapid population growth and significant changes in land-use and infrastructure, leading to over-exploitation of the landscape through irrigation and construction. This dataset uses climate data, satellite imagery, and land cover information to illustrate the substantial increase in construction activity and highlights the intricate relationship between climate change predictions and current socio-political developments in the Levant.
Folder structure
The main folder after download contains all data, in which the following subfolders are stored are stored as zipped files:
“code” stores the above described 9 code chunks to read, extract, process, analyse, and visualize the data.
“MODIS_merged” contains the 16-days, 250 m resolution NDVI imagery merged from three tiles (h20v05, h21v05, h21v06) and cropped to the study area, n=510, covering January 2001 to December 2022 and including January and February 2023.
“mask” contains a single shapefile, which is the merged product of administrative boundaries, including Jordan, Lebanon, Israel, Syria, and Palestine (“MERGED_LEVANT.shp”).
“yield_productivity” contains .csv files of yield information for all countries listed above.
“population” contains two files with the same name but different format. The .csv file is for processing and plotting in R. The .ods file is for enhanced visualization of population dynamics in the Levant (Socio_cultural_political_development_database_FAO2023.ods).
“GLDAS” stores the raw data of the NASA Global Land Data Assimilation System datasets that can be read, extracted (variable name), and processed using code “8_GLDAS_read_extract_trend” from the respective folder. One folder contains data from 1975-2022 and a second the additional January and February 2023 data.
“built_up” contains the landcover and built-up change data from 1975 to 2022. This folder is subdivided into two subfolder which contain the raw data and the already processed data. “raw_data” contains the unprocessed datasets and “derived_data” stores the cropped built_up datasets at 5 year intervals, e.g., “Levant_built_up_1975.tif”.
Code structure
1_MODIS_NDVI_hdf_file_extraction.R
This is the first code chunk that refers to the extraction of MODIS data from .hdf file format. The following packages must be installed and the raw data must be downloaded using a simple mass downloader, e.g., from google chrome. Packages: terra. Download MODIS data from after registration from: https://lpdaac.usgs.gov/products/mod13q1v061/ or https://search.earthdata.nasa.gov/search (MODIS/Terra Vegetation Indices 16-Day L3 Global 250m SIN Grid V061, last accessed, 09th of October 2023). The code reads a list of files, extracts the NDVI, and saves each file to a single .tif-file with the indication “NDVI”. Because the study area is quite large, we have to load three different (spatially) time series and merge them later. Note that the time series are temporally consistent.
2_MERGE_MODIS_tiles.R
In this code, we load and merge the three different stacks to produce large and consistent time series of NDVI imagery across the study area. We further use the package gtools to load the files in (1, 2, 3, 4, 5, 6, etc.). Here, we have three stacks from which we merge the first two (stack 1, stack 2) and store them. We then merge this stack with stack 3. We produce single files named NDVI_final_*consecutivenumber*.tif. Before saving the final output of single merged files, create a folder called “merged” and set the working directory to this folder, e.g., setwd("your directory_MODIS/merged").
3_CROP_MODIS_merged_tiles.R
Now we want to crop the derived MODIS tiles to our study area. We are using a mask, which is provided as .shp file in the repository, named "MERGED_LEVANT.shp". We load the merged .tif files and crop the stack with the vector. Saving to individual files, we name them “NDVI_merged_clip_*consecutivenumber*.tif. We now produced single cropped NDVI time series data from MODIS. The repository provides the already clipped and merged NDVI datasets.
4_TREND_analysis_NDVI.R
Now, we want to perform trend analysis from the derived data. The data we load is tricky as it contains 16-days return period across a year for the period of 22 years. Growing season sums contain MAM (March-May), JJA (June-August), and SON (September-November). December is represented as a single file, which means that the period DJF (December-February) is represented by 5 images instead of 6. For the last DJF period (December 2022), the data from January and February 2023 can be added. The code selects the respective images from the stack, depending on which period is under consideration. From these stacks, individual annually resolved growing season sums are generated and the slope is calculated. We can then extract the p-values of the trend and characterize all values with high confidence level (0.05). Using the ggplot2 package and the melt function from reshape2 package, we can create a plot of the reclassified NDVI trends together with a local smoother (LOESS) of value 0.3.To increase comparability and understand the amplitude of the trends, z-scores were calculated and plotted, which show the deviation of the values from the mean. This has been done for the NDVI values as well as the GLDAS climate variables as a normalization technique.
5_BUILT_UP_change_raster.R
Let us look at the landcover changes now. We are working with the terra package and get raster data from here: https://ghsl.jrc.ec.europa.eu/download.php?ds=bu (last accessed 03. March 2023, 100 m resolution, global coverage). Here, one can download the temporal coverage that is aimed for and reclassify it using the code after cropping to the individual study area. Here, I summed up different raster to characterize the built-up change in continuous values between 1975 and 2022.
6_POPULATION_numbers_plot.R
For this plot, one needs to load the .csv-file “Socio_cultural_political_development_database_FAO2023.csv” from the repository. The ggplot script provided produces the desired plot with all countries under consideration.
7_YIELD_plot.R
In this section, we are using the country productivity from the supplement in the repository “yield_productivity” (e.g., "Jordan_yield.csv". Each of the single country yield datasets is plotted in a ggplot and combined using the patchwork package in R.
8_GLDAS_read_extract_trend
The last code provides the basis for the trend analysis of the climate variables used in the paper. The raw data can be accessed https://disc.gsfc.nasa.gov/datasets?keywords=GLDAS%20Noah%20Land%20Surface%20Model%20L4%20monthly&page=1 (last accessed 9th of October 2023). The raw data comes in .nc file format and various variables can be extracted using the [“^a variable name”] command from the spatraster collection. Each time you run the code, this variable name must be adjusted to meet the requirements for the variables (see this link for abbreviations: https://disc.gsfc.nasa.gov/datasets/GLDAS_CLSM025_D_2.0/summary, last accessed 09th of October 2023; or the respective code chunk when reading a .nc file with the ncdf4 package in R) or run print(nc) from the code or use names(the spatraster collection). Choosing one variable, the code uses the MERGED_LEVANT.shp mask from the repository to crop and mask the data to the outline of the study area.From the processed data, trend analysis are conducted and z-scores were calculated following the code described above. However, annual trends require the frequency of the time series analysis to be set to value = 12. Regarding, e.g., rainfall, which is measured as annual sums and not means, the chunk r.sum=r.sum/12 has to be removed or set to r.sum=r.sum/1 to avoid calculating annual mean values (see other variables). Seasonal subset can be calculated as described in the code. Here, 3-month subsets were chosen for growing seasons, e.g. March-May (MAM), June-July (JJA), September-November (SON), and DJF (December-February, including Jan/Feb of the consecutive year).From the data, mean values of 48 consecutive years are calculated and trend analysis are performed as describe above. In the same way, p-values are extracted and 95 % confidence level values are marked with dots on the raster plot. This analysis can be performed with a much longer time series, other variables, ad different spatial extent across the globe due to the availability of the GLDAS variables.
(9_workflow_diagramme) this simple code can be used to plot a workflow diagram and is detached from the actual analysis.
Conceptualization, Methodology, Software, Validation, Formal analysis, Investigation, Resources, Data Curation, Writing - Original Draft, Writing - Review & Editing, Visualization, Supervision, Project administration, and Funding acquisition: Michael
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This dataset is retrieved from the user Mobius page, where it's generated by respondents to a distributed survey via Amazon Mechanical Turk between 03.12.2016-05.12.2016. I woıuld like to thank Möbius and everyone responsible for the work.
Bellabeat Case Study 1 2022-11-14 1. Introduction Hello everyone, my name is Nur Simais and this project is part of Google Data Analytics Professional Certificate. There have been multiple skills and skillsets learned throughout this course that can mainly be categorized under soft and hard skills. Also, this case study I have chosen is about the company calles “Bellabeat”, a fitness tracker device. The company is founded in 2013 by Urška Sršen and Sando Mur. It gradually gained recognition and expanded in many countires.(https://bellabeat.com/) Adding this brief info about the company, I’d like to say that doing the business analysis will help the company to see how it can achieve it’s goals and what can be done as to improve more.
During the analysis process, I will be using the Google’s “Ask-Prepare-Process-Analyze-Share-Act” Framework that I have learned throughout this certification and apply the tools and skillsets into it.
1.ASK
1.1 Business Task The goal of this project is to analyze smart device usage data in order to gain insight into how consumers use non-Bellabeat smart devices and how to apply these insights into Bellabeat’s marketing strategy using these three questions:
What are some trends in smart device usage? How could these trends apply to Bellabeat customers? How could these trends help influence Bellabeat marketing strategy?
2.PREPARE Prepare the Data and Libraries in RStudio Collect the data required for analysis but since the data is available on Kaggle publicly, FitBit Fitness Tracker Data (CC0: Public Domain) and download the dataset.
There are 18 packages but after examining the excel docs, I decided to use these 8 datasets: dailyActivity_merged.csv, heartrate_seconds_merged.csv, hourlyCalories_merged.csv, hourlyIntensities_merged.csv, hourlySteps_merged.csv, minuteMETsNarrow_merged.csv, sleepDay_merged.csv, weightLogInfo_merged.csv 2.1 Install and load the packages Install the RStudio libraries for analysis and visualizations
install.packages("tidyverse") # core package for cleaning and analysis
install.packages("lubridate") # date library mdy()
install.packages("janitor") # clean_names() to consists only _, character, numbers, and letters.
install.packages("dplyr") #helps to check the garmmar of data manioulation
Load the libraries
library(tidyverse)
library(janitor) ##
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library(lubridate)
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library(dplyr) Having loaded tidyverse package, the rest of the essential packages (ggplot2, dplyr, and tidyr) are loaded as well.
2.2 Importing and Preparing the Dataset Upload the archived dataset to RStudio by clicking the Upload button in the bottom right pane.
File will be saved in a new folder named “Fitabase Data 4.12.16-5.12.16”. Importing the datasets and renaming them.
daily_activity <- read.csv("dailyActivity_merged.csv") heartrate_seconds <- read_csv("heartrate_seconds_merged.csv")
##
spec() to retrieve the full column specification for this data.show_col_types = FALSE to quiet this message.hourly_calories <- read_csv("hourlyCalories_merged.csv")
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The Online Retail Sales Dataset, often referred to as the Online Retail.csv file, is an extensive and comprehensive collection of data points relating to e-commerce transactions. This dataset provides a detailed view of sales activities within the online retail sector, covering numerous essential attributes necessary for a quantitative understanding of consumer behavior and the overall business performance.
One of the key elements covered in this dataset is 'InvoiceNo', which is a unique identifier for each transaction taking place in this retail environment. Given its uniqueness, it serves as a primary key for distinguishing individual transactions. It's worthwhile to note that these Invoice Numbers are numerical values.
Another important attribute included here is 'StockCode'. Each product listed or sold on this online retail platform has been assigned with its unique identification code or StockCode. These codes are also numerical values that offer another layer to clearly classify items and distinguish one from another.
For further understanding, every product comes with a basic description noted under the 'Description' column. In textual form, these descriptions provide insights into what exactly each product item entails. Aside from aiding identification efforts, they can potentially open avenues for text-based analysis such as sentiment analysis or keyword flagging based on product trends.
'Moving onto details about transactions themselves', we have two crucial columns: 'Quantity' and 'UnitPrice'. As their names suggest, these show respectively how many particular units of an item were sold per transaction and at what price per unit was sold at.
Further adding detail to our transactions information comes 'InvoiceDate', which records when each separate purchase occurred down to accurate date & time records. This data can be pivotal in recognizing sales patterns throughout different periods or predicting future trends based on historical timing behavior.
Finally yet importantly comes our global indicator - The ‘Country’ column specifies various countries where customers reside who interacts with this particular online platform regularly by making purchases. This application allows us insights into the geographical dispersion of user base across various countries, potentially providing us insights into regional preferences or global market segmentation.
Ith such a wealth of detailed transaction records and customer information, the Online Retail.csv dataset stands as an invaluable tool for those looking to delve deep into online retail sales data analysis. The possibilities with this dataset are vast, ranging from shaping efficient marketing strategies based on geographical data to predicting sales & growth metrics using historical behavior and much more
Here's how to make best use of this dataset:
Getting Started Before you start analyzing your data – you'll have to load it into statistical software such as Python (using pandas library) or R. The dataset is saved in .csv file format which supports easy reading into most data manipulation software.
Understand The Fields
InvoiceNo: Each transaction made has an associated unique numerical identifier called InvoiceNo. Consider it like a receipt code - these allow for tracking individual transactions.
StockCode: To identify each product uniquely during analysis, refer to each StockCode value which is essentially a product identification code.
Description: A brief textual description about each product that can be invaluable when dealing with categories for market-basket type analysis.
Quantity: Each row lists out how many units of a particular item were involved in a single transaction - watch out for very large values as they might represent bulk orders.
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TwitterThis dataset contains files reconstructing single-cell data presented in 'Reference transcriptomics of porcine peripheral immune cells created through bulk and single-cell RNA sequencing' by Herrera-Uribe & Wiarda et al. 2021. Samples of peripheral blood mononuclear cells (PBMCs) were collected from seven pigs and processed for single-cell RNA sequencing (scRNA-seq) in order to provide a reference annotation of porcine immune cell transcriptomics at enhanced, single-cell resolution. Analysis of single-cell data allowed identification of 36 cell clusters that were further classified into 13 cell types, including monocytes, dendritic cells, B cells, antibody-secreting cells, numerous populations of T cells, NK cells, and erythrocytes. Files may be used to reconstruct the data as presented in the manuscript, allowing for individual query by other users. Scripts for original data analysis are available at https://github.com/USDA-FSEPRU/PorcinePBMCs_bulkRNAseq_scRNAseq. Raw data are available at https://www.ebi.ac.uk/ena/browser/view/PRJEB43826. Funding for this dataset was also provided by NRSP8: National Animal Genome Research Program (https://www.nimss.org/projects/view/mrp/outline/18464). Resources in this dataset:Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - All Cells 10X Format. File Name: PBMC7_AllCells.zipResource Description: Zipped folder containing PBMC counts matrix, gene names, and cell IDs. Files are as follows: matrix of gene counts* (matrix.mtx.gx) gene names (features.tsv.gz) cell IDs (barcodes.tsv.gz) *The ‘raw’ count matrix is actually gene counts obtained following ambient RNA removal. During ambient RNA removal, we specified to calculate non-integer count estimations, so most gene counts are actually non-integer values in this matrix but should still be treated as raw/unnormalized data that requires further normalization/transformation. Data can be read into R using the function Read10X().Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - All Cells Metadata. File Name: PBMC7_AllCells_meta.csvResource Description: .csv file containing metadata for cells included in the final dataset. Metadata columns include: nCount_RNA = the number of transcripts detected in a cell nFeature_RNA = the number of genes detected in a cell Loupe = cell barcodes; correspond to the cell IDs found in the .h5Seurat and 10X formatted objects for all cells prcntMito = percent mitochondrial reads in a cell Scrublet = doublet probability score assigned to a cell seurat_clusters = cluster ID assigned to a cell PaperIDs = sample ID for a cell celltypes = cell type ID assigned to a cellResource Title: Herrera-Uribe & Wiarda et al. PBMCs - All Cells PCA Coordinates. File Name: PBMC7_AllCells_PCAcoord.csvResource Description: .csv file containing first 100 PCA coordinates for cells. Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - All Cells t-SNE Coordinates. File Name: PBMC7_AllCells_tSNEcoord.csvResource Description: .csv file containing t-SNE coordinates for all cells.Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - All Cells UMAP Coordinates. File Name: PBMC7_AllCells_UMAPcoord.csvResource Description: .csv file containing UMAP coordinates for all cells.Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - CD4 T Cells t-SNE Coordinates. File Name: PBMC7_CD4only_tSNEcoord.csvResource Description: .csv file containing t-SNE coordinates for only CD4 T cells (clusters 0, 3, 4, 28). A dataset of only CD4 T cells can be re-created from the PBMC7_AllCells.h5Seurat, and t-SNE coordinates used in publication can be re-assigned using this .csv file.Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - CD4 T Cells UMAP Coordinates. File Name: PBMC7_CD4only_UMAPcoord.csvResource Description: .csv file containing UMAP coordinates for only CD4 T cells (clusters 0, 3, 4, 28). A dataset of only CD4 T cells can be re-created from the PBMC7_AllCells.h5Seurat, and UMAP coordinates used in publication can be re-assigned using this .csv file.Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - Gamma Delta T Cells UMAP Coordinates. File Name: PBMC7_GDonly_UMAPcoord.csvResource Description: .csv file containing UMAP coordinates for only gamma delta T cells (clusters 6, 21, 24, 31). A dataset of only gamma delta T cells can be re-created from the PBMC7_AllCells.h5Seurat, and UMAP coordinates used in publication can be re-assigned using this .csv file.Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - Gamma Delta T Cells t-SNE Coordinates. File Name: PBMC7_GDonly_tSNEcoord.csvResource Description: .csv file containing t-SNE coordinates for only gamma delta T cells (clusters 6, 21, 24, 31). A dataset of only gamma delta T cells can be re-created from the PBMC7_AllCells.h5Seurat, and t-SNE coordinates used in publication can be re-assigned using this .csv file.Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - Gene Annotation Information. File Name: UnfilteredGeneInfo.txtResource Description: .txt file containing gene nomenclature information used to assign gene names in the dataset. 'Name' column corresponds to the name assigned to a feature in the dataset.Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - All Cells H5Seurat. File Name: PBMC7.tarResource Description: .h5Seurat object of all cells in PBMC dataset. File needs to be untarred, then read into R using function LoadH5Seurat().
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The retailer wants to target customers with suggestions on itemset that a customer is most likely to purchase .I was given dataset contains data of a retailer; the transaction data provides data around all the transactions that have happened over a period of time. Retailer will use result to grove in his industry and provide for customer suggestions on itemset, we be able increase customer engagement and improve customer experience and identify customer behavior. I will solve this problem with use Association Rules type of unsupervised learning technique that checks for the dependency of one data item on another data item.
Association Rule is most used when you are planning to build association in different objects in a set. It works when you are planning to find frequent patterns in a transaction database. It can tell you what items do customers frequently buy together and it allows retailer to identify relationships between the items.
Assume there are 100 customers, 10 of them bought Computer Mouth, 9 bought Mat for Mouse and 8 bought both of them. - bought Computer Mouth => bought Mat for Mouse - support = P(Mouth & Mat) = 8/100 = 0.08 - confidence = support/P(Mat for Mouse) = 0.08/0.09 = 0.89 - lift = confidence/P(Computer Mouth) = 0.89/0.10 = 8.9 This just simple example. In practice, a rule needs the support of several hundred transactions, before it can be considered statistically significant, and datasets often contain thousands or millions of transactions.
Number of Attributes: 7
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First, we need to load required libraries. Shortly I describe all libraries.
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Next, we need to upload Assignment-1_Data. xlsx to R to read the dataset.Now we can see our data in R.
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After we will clear our data frame, will remove missing values.
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To apply Association Rule mining, we need to convert dataframe into transaction data to make all items that are bought together in one invoice will be in ...
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Dataset generated by our Microwell-seq 3.0 technique.
Files: In order to save space, we've packaged our data into tar.gz format. Please unzip the files once you've successfully downloaded. RNA_WT_RData.tar.gz: Seurat object along with a metadata including cell barcodes, tissue source & cell type annotation, could be loaded into R environment and used directly. RNA_Tumor_RData.tar.gz: Seurat object along with a metadata including cell barcodes, tissue source, cell type annotation & potential cell state prediction(neoplastic, intermediate & non-neoplastic), could be loaded into R environment and used directly. RNA_WT_Dge.tar.gz: Digital Expression data (in .csv format) generated by Drop-seq tools, with batch effect removed by customed scripts. RNA_Tumor_Dge.tar.gz : Digital Expression data(in .csv format) generated by Drop-seq tools, with batch effect removed by customed scripts. ATAC_WT_SparseMatrix.tar.gz: scATAC-seq data in 10X-like format(matrix.mtx, barcodes.csv, features.csv), along with a metadata including cell barcodes, tissue source & cell type annotation. ATAC_Tumor_SparseMatrix.tar.gz: scATAC-seq data in 10X-like format(matrix.mtx, barcodes.csv, features.csv), along with a metadata including cell barcodes, tissue source, cell type annotation & potential cell state prediction(neoplastic, intermediate & non-neoplastic).
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This data repository feeds into the meta-repository setup for post-processing of GCAM-SAM outputs. GitHub link of meta-repository is: https://github.com/JGCRI/Kyle-etal_2022_EF
Folders: model/ is the static version of the model used to simulate 8 scenarios. See the GitHub GCAM-SAM repository to follow active development of this model. inputs/ folder contains input datasets and scripts used to prepare files while postprocessing. This is to be used with GitHub post-processing meta-repository. outdata/ contains GCAM-SAM output and post-processed output files used to plot figures.
Key files: SAM-matrix.dat is the consolidated GCAM-SAM output. Use proj_load.R in the metarepo to read the file. region_vals.csv has all 8 indicators in all 8 scenarios for years 2020 till 2100 on a 10 year time step.
Short introduction to the study:
In this paper sustainable agriculture matrix (SAM) is estimated to 2100 using Global Change Analysis Model (GCAM). We model combinatorial variations of yield intensification, dietary shift, and greenhouse gas mitigation scenarios. Findings include scenarios having significant tradeoffs across multiple environmental, economic, and social dimensions. Assessment of these multi-dimensional tradeoffs in a consistent framework improves the quality of information for decision-making.
Should you have any questions, feel free to reach out Page Kyle at pkyle@pnnl.gov.
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Objective: To develop a clinical informatics pipeline designed to capture large-scale structured EHR data for a national patient registry.
Materials and Methods: The EHR-R-REDCap pipeline is implemented using R-statistical software to remap and import structured EHR data into the REDCap-based multi-institutional Merkel Cell Carcinoma (MCC) Patient Registry using an adaptable data dictionary.
Results: Clinical laboratory data were extracted from EPIC Clarity across several participating institutions. Labs were transformed, remapped and imported into the MCC registry using the EHR labs abstraction (eLAB) pipeline. Forty-nine clinical tests encompassing 482,450 results were imported into the registry for 1,109 enrolled MCC patients. Data-quality assessment revealed highly accurate, valid labs. Univariate modeling was performed for labs at baseline on overall survival (N=176) using this clinical informatics pipeline.
Conclusion: We demonstrate feasibility of the facile eLAB workflow. EHR data is successfully transformed, and bulk-loaded/imported into a REDCap-based national registry to execute real-world data analysis and interoperability.
Methods eLAB Development and Source Code (R statistical software):
eLAB is written in R (version 4.0.3), and utilizes the following packages for processing: DescTools, REDCapR, reshape2, splitstackshape, readxl, survival, survminer, and tidyverse. Source code for eLAB can be downloaded directly (https://github.com/TheMillerLab/eLAB).
eLAB reformats EHR data abstracted for an identified population of patients (e.g. medical record numbers (MRN)/name list) under an Institutional Review Board (IRB)-approved protocol. The MCCPR does not host MRNs/names and eLAB converts these to MCCPR assigned record identification numbers (record_id) before import for de-identification.
Functions were written to remap EHR bulk lab data pulls/queries from several sources including Clarity/Crystal reports or institutional EDW including Research Patient Data Registry (RPDR) at MGB. The input, a csv/delimited file of labs for user-defined patients, may vary. Thus, users may need to adapt the initial data wrangling script based on the data input format. However, the downstream transformation, code-lab lookup tables, outcomes analysis, and LOINC remapping are standard for use with the provided REDCap Data Dictionary, DataDictionary_eLAB.csv. The available R-markdown ((https://github.com/TheMillerLab/eLAB) provides suggestions and instructions on where or when upfront script modifications may be necessary to accommodate input variability.
The eLAB pipeline takes several inputs. For example, the input for use with the ‘ehr_format(dt)’ single-line command is non-tabular data assigned as R object ‘dt’ with 4 columns: 1) Patient Name (MRN), 2) Collection Date, 3) Collection Time, and 4) Lab Results wherein several lab panels are in one data frame cell. A mock dataset in this ‘untidy-format’ is provided for demonstration purposes (https://github.com/TheMillerLab/eLAB).
Bulk lab data pulls often result in subtypes of the same lab. For example, potassium labs are reported as “Potassium,” “Potassium-External,” “Potassium(POC),” “Potassium,whole-bld,” “Potassium-Level-External,” “Potassium,venous,” and “Potassium-whole-bld/plasma.” eLAB utilizes a key-value lookup table with ~300 lab subtypes for remapping labs to the Data Dictionary (DD) code. eLAB reformats/accepts only those lab units pre-defined by the registry DD. The lab lookup table is provided for direct use or may be re-configured/updated to meet end-user specifications. eLAB is designed to remap, transform, and filter/adjust value units of semi-structured/structured bulk laboratory values data pulls from the EHR to align with the pre-defined code of the DD.
Data Dictionary (DD)
EHR clinical laboratory data is captured in REDCap using the ‘Labs’ repeating instrument (Supplemental Figures 1-2). The DD is provided for use by researchers at REDCap-participating institutions and is optimized to accommodate the same lab-type captured more than once on the same day for the same patient. The instrument captures 35 clinical lab types. The DD serves several major purposes in the eLAB pipeline. First, it defines every lab type of interest and associated lab unit of interest with a set field/variable name. It also restricts/defines the type of data allowed for entry for each data field, such as a string or numerics. The DD is uploaded into REDCap by every participating site/collaborator and ensures each site collects and codes the data the same way. Automation pipelines, such as eLAB, are designed to remap/clean and reformat data/units utilizing key-value look-up tables that filter and select only the labs/units of interest. eLAB ensures the data pulled from the EHR contains the correct unit and format pre-configured by the DD. The use of the same DD at every participating site ensures that the data field code, format, and relationships in the database are uniform across each site to allow for the simple aggregation of the multi-site data. For example, since every site in the MCCPR uses the same DD, aggregation is efficient and different site csv files are simply combined.
Study Cohort
This study was approved by the MGB IRB. Search of the EHR was performed to identify patients diagnosed with MCC between 1975-2021 (N=1,109) for inclusion in the MCCPR. Subjects diagnosed with primary cutaneous MCC between 2016-2019 (N= 176) were included in the test cohort for exploratory studies of lab result associations with overall survival (OS) using eLAB.
Statistical Analysis
OS is defined as the time from date of MCC diagnosis to date of death. Data was censored at the date of the last follow-up visit if no death event occurred. Univariable Cox proportional hazard modeling was performed among all lab predictors. Due to the hypothesis-generating nature of the work, p-values were exploratory and Bonferroni corrections were not applied.
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Cover crops provide many agroecosystem services, including weed suppression, which is partially exerted through release of allelopathic benzoxazinoid (BX) compounds. This research characterizes (1) changes in concentrations of BX compounds in shoots, roots, and soil at three growth stages (GS) of cereal rye (Secale cereale L.), and (2) their degradation over time following termination. Concentrations of shoot dominant BX compounds, DIBOA-glc and DIBOA, were least at GS 83 (boot). The root dominant BX compound, HMBOA-glc, concentration was least at GS 54 (elongation). Rhizosphere soil BX concentrations were 1000 times smaller than in root tissues. Dominant compounds in soil were HMBOA-glc and HMBOA. Concentrations of BX compounds were similar for soil near root crowns and between-rows. Soil BX concentrations following cereal rye termination declined exponentially over time in three of four treatments: incorporated shoots (S) and roots (R), no-till S+R (cereal rye rolled flat), and no-till R (shoots removed), but not in no-till S. On the day following cereal rye termination, soil concentrations of HMBOA-glc and HMBOA in these three treatments increased above initial concentrations. Concentrations of these two compounds decreased the fastest while DIBOA-glc declined the slowest (half-life of 4 d in no-till S+R soil). Placement of shoots on the surface of an area where cereal rye had not grown (no-till S) did not increase soil concentrations of BX compounds. The short duration and complex dynamics of BX compounds in soil prior to and following termination illustrate the limited window for enhancing weed suppression by cereal rye allelochemicals; valuable information for programs breeding for enhanced weed suppression. In addition to the data analyzed for this article, we also include the R code. Resources in this dataset:Resource Title: BX data following termination. File Name: FinalBXsForMatt-20200908.csvResource Description: For each sample, gives the time, depth, location, and plot treatment, and then the compound concentrations. This is the principal data set analyzed with the R (anal2-cleaned.r) code, see that code for use.Resource Title: BX compounds from 3rd sampling time before termination. File Name: soil2-20201123.csvResource Description: These data are for comparison with the post termination data. They were taken at the 3rd sampling time (pre-termination), a day prior to termination. Each sample is identified with a treatment, date, and plot location, in addition to the BX concentrations. See R code (anal2-cleaned.r) for how this file is used.Resource Title: Soil location (within row versus between row) values of BX compounds. File Name: s2b.csvResource Description: Each row gives the average BX compound for each soil location (within row versus between row) for the second sample for each plot. These data are combined with bx3 (the data set read in from the file , "FinalBXsForMatt-20200908.csv"). See R (anal2-cleaned.r) code for use.Resource Title: R code for analysis of the decay (post-termination) BX data.. File Name: anal2-cleaned.rResource Description: This is the R code used to analyze the termination data. It also creates and writes out some data subsets (used for analysis and plots) that are later read in.Resource Software Recommended: R version 3.6.3,url: https://www.R-project.org/ Resource Title: Tissue BX compounds. File Name: tissues20210728b.csvResource Description: Data file holding results from a tissue analysis for BX compounds, in ug, from shoots and roots, and at various sampling times. Read into the R file, anal1-cleaned.r where it is used in a statistical analysis and to create figures.Resource Title: BX compounds from soil with a live rye cover crop. File Name: soil2-20201214.csvResource Description: BX compounds (in ng/g dry wt), by treatment, sampling time, date, and plot ID. These are data are read into the R program, anal1-cleaned.r, for analysis and to create figures. These are soil samples taken from locations with a live rye plant cover crop.Resource Title: R code for BX analyses of soil under rye and plant tissues. File Name: anal1-cleaned.rResource Description: R code for analysis of the soil BX compounds under a live rye cover crop at different growing stages, and for the analysis of tissue BX compounds. In addition to statistical analyses, code in this file creates figures, also some statistical output that is used to create a file that is later read in for figure creation (s2-CLD20220730-Stage.csv).Resource Software Recommended: R version 3.6.3,url: https://www.R-project.org/ Resource Title: Description of data files for anal2-cleaned.r. File Name: readme2.txtResource Description: Describes the input files used in the R code in anal2-cleaned.r, including descriptions and formats for each field. The file also describes some output (results) files that were uploaded to this site. This is a plain ASCII text file.Resource Title: Estimates produced by anal2-cleaned.r from statistical modeling.. File Name: Estimates20201110.csvResource Description: Estimates produced by anal2-cleaned.r from statistical modeling (see readme2.txt)Resource Title: Summary statistics from anal2-cleaned.r. File Name: CV20210412.csvResource Description: Summary statistics from anal2-cleaned.r, used for plotsResource Title: Data summaries (same as CV20210412.csv), rescaled. File Name: RESCALE-20210412.csvResource Description: Same as "CV20210412.csv" except log of data have been rescaled to minimum at least zero and maximum one, see readme2.txtResource Title: Statistical summaries for different stages. File Name: s2-CLD20220730-Stage.csvResource Description: Statistical summaries used for creating a figure (not used in paper), used in anal1-cleaned.r; data for soil BX under living rye.Resource Title: Description of data files for anal1-cleaned.r. File Name: readme1.txtResource Description: Contains general descriptions of data imported into anal1-cleaned.r, and a description of each field. Also contains some descriptions of files output by anal1-cleaned.r, used to create tables or figures.
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Explanation/Overview: Corresponding dataset for the analyses and results achieved in the CS Track project in the research line on participation analyses, which is also reported in the publication "Does Volunteer Engagement Pay Off? An Analysis of User Participation in Online Citizen Science Projects", a conference paper for the conference CollabTech 2022: Collaboration Technologies and Social Computing and published as part of the Lecture Notes in Computer Science book series (LNCS,volume 13632) here. The usernames have been anonymised. Purpose: The purpose of this dataset is to provide the basis to reproduce the results reported in the associated deliverable, and in the above-mentioned publication. As such, it does not represent raw data, but rather files that already include certain analysis steps (like calculated degrees or other SNA-related measures), ready for analysis, visualisation and interpretation with R. Relatedness: The data of the different projects was derived from the forums of 7 Zooniverse projects based on similar discussion board features. The projects are: 'Galaxy Zoo', 'Gravity Spy', 'Seabirdwatch', 'Snapshot Wisconsin', 'Wildwatch Kenya', 'Galaxy Nurseries', 'Penguin Watch'. Content: In this Zenodo entry, several files can be found. The structure is as follows (files and folders and descriptions). corresponding_calculations.html Quarto-notebook to view in browser corresponding_calculations.qmd Quarto-notebook to view in RStudio assets data annotations annotations.csv List of annotations made per day for each of the analysed projects comments comments.csv Total list of comments with several data fields (i.e., comment id, text, reply_user_id) rolechanges 478_rolechanges.csv List of roles per user to determine number of role changes 1104_rolechanges.csv ... ... totalnetworkdata Edges 478_edges.csv Network data (edge set) for the given projects (without time slices) 1104_edges.csv ... ... Nodes 478_nodes.csv Network data (node set) for the given projects (without time slices) 1104_nodes.csv ... ... trajectories Network data (edge and node sets) for the given projects and all time slices (Q1 2016 - Q4 2021) 478 Edges edges_4782016_q1.csv edges_4782016_q2.csv edges_4782016_q3.csv edges_4782016_q4.csv ... Nodes nodes_4782016_q1.csv nodes_4782016_q4.csv nodes_4782016_q3.csv nodes_4782016_q2.csv ... 1104 Edges ... Nodes ... ... scripts datavizfuncs.R script for the data visualisation functions, automatically executed from within corresponding_calculations.qmd import.R script for the import of data, automatically executed from within corresponding_calculations.qmd corresponding_calculations_files files for the html/qmd view in the browser/RStudio Grouping: The data is grouped according to given criteria (e.g., project_title or time). Accordingly, the respective files can be found in the data structure
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This project is a collection of files to allow users to reproduce the model development and benchmarking in "Dawnn: single-cell differential abundance with neural networks" (Hall and Castellano, under review). Dawnn is a tool for detecting differential abundance in single-cell RNAseq datasets. It is available as an R package here. Please contact us if you are unable to reproduce any of the analysis in our paper. The files in this collection correspond to the benchmarking dataset based on single-cell RNAseq of mouse emrbyo cells.
FILES: Input data Dataset from: "A single-cell molecular map of mouse gastrulation and early organogenesis". Nature 566, pp490–495 (2019). The input data is loaded from the MouseGastrulationData R package. We upload here the RDS file generated by loading the dataset in process_mouse_cells.R in case the R package becomes unavailable
MouseGastrulationData_loaded_dataset.RDS Dataset loaded from MouseGastrulationData R package in process_mouse_cells.R (in call to EmbryoAtlasData function).
Data processing code
process_mouse_cells.R Generates benchmarking dataset from input data. (Loads input data; Runs the standard single-cell RNAseq pipeline). Follows Dann et al. Resulting dataset saved as mouse_gastrulation_data_regen.RDS. simulate_mouse_pc1_Rscript.R R code to simulate P(Condition_1)s for benchmarking. simulate_mouse_pc1_bash.sh Bash script to execute simulate_mouse_pc1_Rscript.R. Outputs stored in benchmark_dataset_mouse_pc1s_regen.csv. simulate_mouse_labels_Rscript.R R code to simulate labels for benchmarking. simulate_mouse_labels_bash.sh Bash script to execute simulate_mouse_labels_Rscript.R. Outputs stored in benchmark_dataset_mouse.csv.
Resulting datasets
mouse_gastrulation_data_regen.RDS Seurat dataset generated by process_mouse_cells.R. benchmark_dataset_mouse.csv Cell labels generated by simulate_mouse_labels_bash.sh. benchmark_dataset_mouse_pc1s_regen.csv P(Condition_1)s generated by simulate_mouse_pc1_bash.sh.
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Replication pack, FSE2018 submission #164: ------------------------------------------
**Working title:** Ecosystem-Level Factors Affecting the Survival of Open-Source Projects: A Case Study of the PyPI Ecosystem **Note:** link to data artifacts is already included in the paper. Link to the code will be included in the Camera Ready version as well. Content description =================== - **ghd-0.1.0.zip** - the code archive. This code produces the dataset files described below - **settings.py** - settings template for the code archive. - **dataset_minimal_Jan_2018.zip** - the minimally sufficient version of the dataset. This dataset only includes stats aggregated by the ecosystem (PyPI) - **dataset_full_Jan_2018.tgz** - full version of the dataset, including project-level statistics. It is ~34Gb unpacked. This dataset still doesn't include PyPI packages themselves, which take around 2TB. - **build_model.r, helpers.r** - R files to process the survival data (`survival_data.csv` in **dataset_minimal_Jan_2018.zip**, `common.cache/survival_data.pypi_2008_2017-12_6.csv` in **dataset_full_Jan_2018.tgz**) - **Interview protocol.pdf** - approximate protocol used for semistructured interviews. - LICENSE - text of GPL v3, under which this dataset is published - INSTALL.md - replication guide (~2 pages)
Replication guide ================= Step 0 - prerequisites ---------------------- - Unix-compatible OS (Linux or OS X) - Python interpreter (2.7 was used; Python 3 compatibility is highly likely) - R 3.4 or higher (3.4.4 was used, 3.2 is known to be incompatible) Depending on detalization level (see Step 2 for more details): - up to 2Tb of disk space (see Step 2 detalization levels) - at least 16Gb of RAM (64 preferable) - few hours to few month of processing time Step 1 - software ---------------- - unpack **ghd-0.1.0.zip**, or clone from gitlab: git clone https://gitlab.com/user2589/ghd.git git checkout 0.1.0 `cd` into the extracted folder. All commands below assume it as a current directory. - copy `settings.py` into the extracted folder. Edit the file: * set `DATASET_PATH` to some newly created folder path * add at least one GitHub API token to `SCRAPER_GITHUB_API_TOKENS` - install docker. For Ubuntu Linux, the command is `sudo apt-get install docker-compose` - install libarchive and headers: `sudo apt-get install libarchive-dev` - (optional) to replicate on NPM, install yajl: `sudo apt-get install yajl-tools` Without this dependency, you might get an error on the next step, but it's safe to ignore. - install Python libraries: `pip install --user -r requirements.txt` . - disable all APIs except GitHub (Bitbucket and Gitlab support were not yet implemented when this study was in progress): edit `scraper/init.py`, comment out everything except GitHub support in `PROVIDERS`. Step 2 - obtaining the dataset ----------------------------- The ultimate goal of this step is to get output of the Python function `common.utils.survival_data()` and save it into a CSV file: # copy and paste into a Python console from common import utils survival_data = utils.survival_data('pypi', '2008', smoothing=6) survival_data.to_csv('survival_data.csv') Since full replication will take several months, here are some ways to speedup the process: ####Option 2.a, difficulty level: easiest Just use the precomputed data. Step 1 is not necessary under this scenario. - extract **dataset_minimal_Jan_2018.zip** - get `survival_data.csv`, go to the next step ####Option 2.b, difficulty level: easy Use precomputed longitudinal feature values to build the final table. The whole process will take 15..30 minutes. - create a folder `