Pathogen diversity resulting in quasispecies can enable persistence and adaptation to host defenses and therapies. However, accurate quasispecies characterization can be impeded by errors introduced during sample handling and sequencing which can require extensive optimizations to overcome. We present complete laboratory and bioinformatics workflows to overcome many of these hurdles. The Pacific Biosciences single molecule real-time platform was used to sequence PCR amplicons derived from cDNA templates tagged with universal molecular identifiers (SMRT-UMI). Optimized laboratory protocols were developed through extensive testing of different sample preparation conditions to minimize between-template recombination during PCR and the use of UMI allowed accurate template quantitation as well as removal of point mutations introduced during PCR and sequencing to produce a highly accurate consensus sequence from each template. Handling of the large datasets produced from SMRT-UMI sequencing was facilitated by a novel bioinformatic pipeline, Probabilistic Offspring Resolver for Primer IDs (PORPIDpipeline), that automatically filters and parses reads by sample, identifies and discards reads with UMIs likely created from PCR and sequencing errors, generates consensus sequences, checks for contamination within the dataset, and removes any sequence with evidence of PCR recombination or early cycle PCR errors, resulting in highly accurate sequence datasets. The optimized SMRT-UMI sequencing method presented here represents a highly adaptable and established starting point for accurate sequencing of diverse pathogens. These methods are illustrated through characterization of human immunodeficiency virus (HIV) quasispecies. Methods This serves as an overview of the analysis performed on PacBio sequence data that is summarized in Analysis Flowchart.pdf and was used as primary data for the paper by Westfall et al. "Optimized SMRT-UMI protocol produces highly accurate sequence datasets from diverse populations – application to HIV-1 quasispecies" Five different PacBio sequencing datasets were used for this analysis: M027, M2199, M1567, M004, and M005 For the datasets which were indexed (M027, M2199), CCS reads from PacBio sequencing files and the chunked_demux_config files were used as input for the chunked_demux pipeline. Each config file lists the different Index primers added during PCR to each sample. The pipeline produces one fastq file for each Index primer combination in the config. For example, in dataset M027 there were 3–4 samples using each Index combination. The fastq files from each demultiplexed read set were moved to the sUMI_dUMI_comparison pipeline fastq folder for further demultiplexing by sample and consensus generation with that pipeline. More information about the chunked_demux pipeline can be found in the README.md file on GitHub. The demultiplexed read collections from the chunked_demux pipeline or CCS read files from datasets which were not indexed (M1567, M004, M005) were each used as input for the sUMI_dUMI_comparison pipeline along with each dataset's config file. Each config file contains the primer sequences for each sample (including the sample ID block in the cDNA primer) and further demultiplexes the reads to prepare data tables summarizing all of the UMI sequences and counts for each family (tagged.tar.gz) as well as consensus sequences from each sUMI and rank 1 dUMI family (consensus.tar.gz). More information about the sUMI_dUMI_comparison pipeline can be found in the paper and the README.md file on GitHub. The consensus.tar.gz and tagged.tar.gz files were moved from sUMI_dUMI_comparison pipeline directory on the server to the Pipeline_Outputs folder in this analysis directory for each dataset and appended with the dataset name (e.g. consensus_M027.tar.gz). Also in this analysis directory is a Sample_Info_Table.csv containing information about how each of the samples was prepared, such as purification methods and number of PCRs. There are also three other folders: Sequence_Analysis, Indentifying_Recombinant_Reads, and Figures. Each has an .Rmd file with the same name inside which is used to collect, summarize, and analyze the data. All of these collections of code were written and executed in RStudio to track notes and summarize results. Sequence_Analysis.Rmd has instructions to decompress all of the consensus.tar.gz files, combine them, and create two fasta files, one with all sUMI and one with all dUMI sequences. Using these as input, two data tables were created, that summarize all sequences and read counts for each sample that pass various criteria. These are used to help create Table 2 and as input for Indentifying_Recombinant_Reads.Rmd and Figures.Rmd. Next, 2 fasta files containing all of the rank 1 dUMI sequences and the matching sUMI sequences were created. These were used as input for the python script compare_seqs.py which identifies any matched sequences that are different between sUMI and dUMI read collections. This information was also used to help create Table 2. Finally, to populate the table with the number of sequences and bases in each sequence subset of interest, different sequence collections were saved and viewed in the Geneious program. To investigate the cause of sequences where the sUMI and dUMI sequences do not match, tagged.tar.gz was decompressed and for each family with discordant sUMI and dUMI sequences the reads from the UMI1_keeping directory were aligned using geneious. Reads from dUMI families failing the 0.7 filter were also aligned in Genious. The uncompressed tagged folder was then removed to save space. These read collections contain all of the reads in a UMI1 family and still include the UMI2 sequence. By examining the alignment and specifically the UMI2 sequences, the site of the discordance and its case were identified for each family as described in the paper. These alignments were saved as "Sequence Alignments.geneious". The counts of how many families were the result of PCR recombination were used in the body of the paper. Using Identifying_Recombinant_Reads.Rmd, the dUMI_ranked.csv file from each sample was extracted from all of the tagged.tar.gz files, combined and used as input to create a single dataset containing all UMI information from all samples. This file dUMI_df.csv was used as input for Figures.Rmd. Figures.Rmd used dUMI_df.csv, sequence_counts.csv, and read_counts.csv as input to create draft figures and then individual datasets for eachFigure. These were copied into Prism software to create the final figures for the paper.

Replication pack, FSE2018 submission #164:
------------------------------------------

**Working title:** Ecosystem-Level Factors Affecting the Survival of Open-Source Projects: 
A Case Study of the PyPI Ecosystem

**Note:** link to data artifacts is already included in the paper. 
Link to the code will be included in the Camera Ready version as well.


Content description
===================

- **ghd-0.1.0.zip** - the code archive. This code produces the dataset files 
 described below
- **settings.py** - settings template for the code archive.
- **dataset_minimal_Jan_2018.zip** - the minimally sufficient version of the dataset.
 This dataset only includes stats aggregated by the ecosystem (PyPI)
- **dataset_full_Jan_2018.tgz** - full version of the dataset, including project-level
 statistics. It is ~34Gb unpacked. This dataset still doesn't include PyPI packages
 themselves, which take around 2TB.
- **build_model.r, helpers.r** - R files to process the survival data 
  (`survival_data.csv` in **dataset_minimal_Jan_2018.zip**, 
  `common.cache/survival_data.pypi_2008_2017-12_6.csv` in 
  **dataset_full_Jan_2018.tgz**)
- **Interview protocol.pdf** - approximate protocol used for semistructured interviews.
- LICENSE - text of GPL v3, under which this dataset is published
- INSTALL.md - replication guide (~2 pages)

Replication guide
=================

Step 0 - prerequisites
----------------------

- Unix-compatible OS (Linux or OS X)
- Python interpreter (2.7 was used; Python 3 compatibility is highly likely)
- R 3.4 or higher (3.4.4 was used, 3.2 is known to be incompatible)

Depending on detalization level (see Step 2 for more details):
- up to 2Tb of disk space (see Step 2 detalization levels)
- at least 16Gb of RAM (64 preferable)
- few hours to few month of processing time

Step 1 - software
----------------

- unpack **ghd-0.1.0.zip**, or clone from gitlab:

   git clone https://gitlab.com/user2589/ghd.git
   git checkout 0.1.0
 
 `cd` into the extracted folder. 
 All commands below assume it as a current directory.
  
- copy `settings.py` into the extracted folder. Edit the file:
  * set `DATASET_PATH` to some newly created folder path
  * add at least one GitHub API token to `SCRAPER_GITHUB_API_TOKENS` 
- install docker. For Ubuntu Linux, the command is 
  `sudo apt-get install docker-compose`
- install libarchive and headers: `sudo apt-get install libarchive-dev`
- (optional) to replicate on NPM, install yajl: `sudo apt-get install yajl-tools`
 Without this dependency, you might get an error on the next step, 
 but it's safe to ignore.
- install Python libraries: `pip install --user -r requirements.txt` . 
- disable all APIs except GitHub (Bitbucket and Gitlab support were
 not yet implemented when this study was in progress): edit
 `scraper/init.py`, comment out everything except GitHub support
 in `PROVIDERS`.

Step 2 - obtaining the dataset
-----------------------------

The ultimate goal of this step is to get output of the Python function 
`common.utils.survival_data()` and save it into a CSV file:

  # copy and paste into a Python console
  from common import utils
  survival_data = utils.survival_data('pypi', '2008', smoothing=6)
  survival_data.to_csv('survival_data.csv')

Since full replication will take several months, here are some ways to speedup
the process:

####Option 2.a, difficulty level: easiest

Just use the precomputed data. Step 1 is not necessary under this scenario.

- extract **dataset_minimal_Jan_2018.zip**
- get `survival_data.csv`, go to the next step

####Option 2.b, difficulty level: easy

Use precomputed longitudinal feature values to build the final table.
The whole process will take 15..30 minutes.

- create a folder `

Each R script replicates all of the example code from one chapter from the book. All required data for each script are also uploaded, as are all data used in the practice problems at the end of each chapter. The data are drawn from a wide array of sources, so please cite the original work if you ever use any of these data sets for research purposes.

The data represent web-scraping of hyperlinks from a selection of environmental stewardship organizations that were identified in the 2017 NYC Stewardship Mapping and Assessment Project (STEW-MAP) (USDA 2017). There are two data sets: 1) the original scrape containing all hyperlinks within the websites and associated attribute values (see "README" file); 2) a cleaned and reduced dataset formatted for network analysis. For dataset 1: Organizations were selected from from the 2017 NYC Stewardship Mapping and Assessment Project (STEW-MAP) (USDA 2017), a publicly available, spatial data set about environmental stewardship organizations working in New York City, USA (N = 719). To create a smaller and more manageable sample to analyze, all organizations that intersected (i.e., worked entirely within or overlapped) the NYC borough of Staten Island were selected for a geographically bounded sample. Only organizations with working websites and that the web scraper could access were retained for the study (n = 78). The websites were scraped between 09 and 17 June 2020 to a maximum search depth of ten using the snaWeb package (version 1.0.1, Stockton 2020) in the R computational language environment (R Core Team 2020). For dataset 2: The complete scrape results were cleaned, reduced, and formatted as a standard edge-array (node1, node2, edge attribute) for network analysis. See "READ ME" file for further details. References: R Core Team. (2020). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL https://www.R-project.org/. Version 4.0.3. Stockton, T. (2020). snaWeb Package: An R package for finding and building social networks for a website, version 1.0.1. USDA Forest Service. (2017). Stewardship Mapping and Assessment Project (STEW-MAP). New York City Data Set. Available online at https://www.nrs.fs.fed.us/STEW-MAP/data/. This dataset is associated with the following publication: Sayles, J., R. Furey, and M. Ten Brink. How deep to dig: effects of web-scraping search depth on hyperlink network analysis of environmental stewardship organizations. Applied Network Science. Springer Nature, New York, NY, 7: 36, (2022).

analyze the health and retirement study (hrs) with r the hrs is the one and only longitudinal survey of american seniors. with a panel starting its third decade, the current pool of respondents includes older folks who have been interviewed every two years as far back as 1992. unlike cross-sectional or shorter panel surveys, respondents keep responding until, well, death d o us part. paid for by the national institute on aging and administered by the university of michigan's institute for social research, if you apply for an interviewer job with them, i hope you like werther's original. figuring out how to analyze this data set might trigger your fight-or-flight synapses if you just start clicking arou nd on michigan's website. instead, read pages numbered 10-17 (pdf pages 12-19) of this introduction pdf and don't touch the data until you understand figure a-3 on that last page. if you start enjoying yourself, here's the whole book. after that, it's time to register for access to the (free) data. keep your username and password handy, you'll need it for the top of the download automation r script. next, look at this data flowchart to get an idea of why the data download page is such a righteous jungle. but wait, good news: umich recently farmed out its data management to the rand corporation, who promptly constructed a giant consolidated file with one record per respondent across the whole panel. oh so beautiful. the rand hrs files make much of the older data and syntax examples obsolete, so when you come across stuff like instructions on how to merge years, you can happily ignore them - rand has done it for you. the health and retirement study only includes noninstitutionalized adults when new respondents get added to the panel (as they were in 1992, 1993, 1998, 2004, and 2010) but once they're in, they're in - respondents have a weight of zero for interview waves when they were nursing home residents; but they're still responding and will continue to contribute to your statistics so long as you're generalizing about a population from a previous wave (for example: it's possible to compute "among all americans who were 50+ years old in 1998, x% lived in nursing homes by 2010"). my source for that 411? page 13 of the design doc. wicked. this new github repository contains five scripts: 1992 - 2010 download HRS microdata.R loop through every year and every file, download, then unzip everything in one big party impor t longitudinal RAND contributed files.R create a SQLite database (.db) on the local disk load the rand, rand-cams, and both rand-family files into the database (.db) in chunks (to prevent overloading ram) longitudinal RAND - analysis examples.R connect to the sql database created by the 'import longitudinal RAND contributed files' program create tw o database-backed complex sample survey object, using a taylor-series linearization design perform a mountain of analysis examples with wave weights from two different points in the panel import example HRS file.R load a fixed-width file using only the sas importation script directly into ram with < a href="http://blog.revolutionanalytics.com/2012/07/importing-public-data-with-sas-instructions-into-r.html">SAScii parse through the IF block at the bottom of the sas importation script, blank out a number of variables save the file as an R data file (.rda) for fast loading later replicate 2002 regression.R connect to the sql database created by the 'import longitudinal RAND contributed files' program create a database-backed complex sample survey object, using a taylor-series linearization design exactly match the final regression shown in this document provided by analysts at RAND as an update of the regression on pdf page B76 of this document . click here to view these five scripts for more detail about the health and retirement study (hrs), visit: michigan's hrs homepage rand's hrs homepage the hrs wikipedia page a running list of publications using hrs notes: exemplary work making it this far. as a reward, here's the detailed codebook for the main rand hrs file. note that rand also creates 'flat files' for every survey wave, but really, most every analysis you c an think of is possible using just the four files imported with the rand importation script above. if you must work with the non-rand files, there's an example of how to import a single hrs (umich-created) file, but if you wish to import more than one, you'll have to write some for loops yourself. confidential to sas, spss, stata, and sudaan users: a tidal wave is coming. you can get water up your nose and be dragged out to sea, or you can grab a surf board. time to transition to r. :D

The objective behind attempting this dataset was to understand the predictors that contribute to the life expectancy around the world. I have used Linear Regression, Decision Tree and Random Forest for this purpose. Steps Involved: - Read the csv file - Data Cleaning: - Variables Country and Status were showing as having character data types. These had to be converted to factor - 2563 missing values were encountered with Population variable having the most of the missing values i.e 652 - Missing rows were dropped before we could run the analysis. 3) Run Linear Regression - Before running linear regression, 3 variables were dropped as they were not found to be having that much of an effect on the dependent variable i.e Life Expectancy. These 3 variables were Country, Year & Status. This meant we are now working with 19 variables (1 dependent and 18 independent variables) - We run the linear regression. Multiple R squared is 83% which means that independent variables can explain 83% change or variance in the dependent variable. - OULTLIER DETECTION. We check for outliers using IQR and find 54 outliers. These outliers are then removed before we run the regression analysis once again. Multiple R squared increased from 83% to 86%. - MULTICOLLINEARITY. We check for multicollinearity using the VIF model(Variance Inflation Factor). This is being done in case when two or more independent variables showing high correlation. The thumb rule is that absolute VIF values above 5 should be removed. We find 6 variables that have a VIF value higher than 5 namely Infant.deaths, percentage.expenditure,Under.five.deaths,GDP,thinness1.19,thinness5.9. Infant deaths and Under Five deaths have strong collinearity so we drop infant deaths(which has the higher VIF value). - When we run the linear regression model again, VIF value of Under.Five.Deaths goes down from 211.46 to 2.74 while the other variable's VIF values reduce very less. Variable thinness1.19 is now dropped and we run the regression once more. - Variable thinness5.9 whose absolute VIF value was 7.61 has now dropped to 1.95. GDP and Population are still having VIF value more than 5 but I decided against dropping these as I consider them to be important independent variables. - SET THE SEED AND SPLIT THE DATA INTO TRAIN AND TEST DATA. We run the train data and get multiple R squared of 86% and p value less than that of alpha which states that it is statistically significant. We use the train data to predict the test data to find out the RMSE and MAPE. We run the library(Metrics) for this purpose. - In Linear Regression, RMSE (Root Mean Squared Error) is 3.2. This indicates that on an average, the predicted values have an error of 3.2 years as compared to the actual life expectancy values. - MAPE (Mean Absolute Percentage Error) is 0.037. This indicates an accuracy prediction of 96.20% (1-0.037). - MAE (Mean Absolute Error) is 2.55. This indicates that on an average, the predicted values deviate by approximately 2.83 years from the actual values.

We use DECISION TREE MODEL for the analysis.

• Run the required libraries (rpart, rpart.plot, RColorBrewer, rattle).
• We run the decision tree analysis using rpart and plot the tree. We use fancyRpartPlot.
• We use 5 fold cross validation method with CP (complexity parameter) being 0.01.
• In Decision Tree , RMSE (Root Mean Squared Error) is 3.06. This indicates that on an average, the predicted values have an error of 3.06 years as compared to the actual life expectancy values.
• MAPE (Mean Absolute Percentage Error) is 0.035. This indicates an accuracy prediction of 96.45% (1-0.035).
• MAE (Mean Absolute Error) is 2.35. This indicates that on an average, the predicted values deviate by approximately 2.35 years from the actual values.

We use RANDOM FOREST for the analysis.

• Run library(randomForest)
• We use varImpPlot to find out which variables are most significant and least significant. Income composition is the most important followed by adult mortality and the least relevant independent variable is Population.
• Predict Life expectancy through random forest model.
• In Random Forest , RMSE (Root Mean Squared Error) is 1.73. This indicates that on an average, the predicted values have an error of 1.73 years as compared to the actual life expectancy values.
• MAPE (Mean Absolute Percentage Error) is 0.01. This indicates an accuracy prediction of 98.27% (1-0.01).
• MAE (Mean Absolute Error) is 1.14. This indicates that on an average, the predicted values deviate by approximately 1.14 years from the actual values.

Conclusion: Random Forest is the best model for predicting the life expectancy values as it has the lowest RMSE, MAPE and MAE.

Biological processes exhibit complex temporal dependencies due to the sequential nature of allocation decisions in organisms’ life-cycles, feedback loops, and two-way causality. Consequently, longitudinal data often contain cross-lags: the predictor variable depends on the response variable of the previous time-step. Although statisticians have warned that regression models that ignore such covariate endogeneity in time series are likely to be inappropriate, this has received relatively little attention in biology. Furthermore, the resulting degree of estimation bias remains largely unexplored.

We use a graphical model and numerical simulations to understand why and how regression models that ignore cross-lags can be biased, and how this bias depends on the length and number of time series. Ecological and evolutionary examples are provided to illustrate that cross-lags may be more common than is typically appreciated and that they occur in functionally different ways.

We show that rou...

This dataset includes all the data and R code needed to reproduce the analyses in a forthcoming manuscript:Copes, W. E., Q. D. Read, and B. J. Smith. Environmental influences on drying rate of spray applied disinfestants from horticultural production services. PhytoFrontiers, DOI pending.Study description: Instructions for disinfestants typically specify a dose and a contact time to kill plant pathogens on production surfaces. A problem occurs when disinfestants are applied to large production areas where the evaporation rate is affected by weather conditions. The common contact time recommendation of 10 min may not be achieved under hot, sunny conditions that promote fast drying. This study is an investigation into how the evaporation rates of six commercial disinfestants vary when applied to six types of substrate materials under cool to hot and cloudy to sunny weather conditions. Initially, disinfestants with low surface tension spread out to provide 100% coverage and disinfestants with high surface tension beaded up to provide about 60% coverage when applied to hard smooth surfaces. Disinfestants applied to porous materials were quickly absorbed into the body of the material, such as wood and concrete. Even though disinfestants evaporated faster under hot sunny conditions than under cool cloudy conditions, coverage was reduced considerably in the first 2.5 min under most weather conditions and reduced to less than or equal to 50% coverage by 5 min. Dataset contents: This dataset includes R code to import the data and fit Bayesian statistical models using the model fitting software CmdStan, interfaced with R using the packages brms and cmdstanr. The models (one for 2022 and one for 2023) compare how quickly different spray-applied disinfestants dry, depending on what chemical was sprayed, what surface material it was sprayed onto, and what the weather conditions were at the time. Next, the statistical models are used to generate predictions and compare mean drying rates between the disinfestants, surface materials, and weather conditions. Finally, tables and figures are created. These files are included:Drying2022.csv: drying rate data for the 2022 experimental runWeather2022.csv: weather data for the 2022 experimental runDrying2023.csv: drying rate data for the 2023 experimental runWeather2023.csv: weather data for the 2023 experimental rundisinfestant_drying_analysis.Rmd: RMarkdown notebook with all data processing, analysis, and table creation codedisinfestant_drying_analysis.html: rendered output of notebookMS_figures.R: additional R code to create figures formatted for journal requirementsfit2022_discretetime_weather_solar.rds: fitted brms model object for 2022. This will allow users to reproduce the model prediction results without having to refit the model, which was originally fit on a high-performance computing clusterfit2023_discretetime_weather_solar.rds: fitted brms model object for 2023data_dictionary.xlsx: descriptions of each column in the CSV data files

Overview

This dataset is the repository for the following paper submitted to Data in Brief:

Kempf, M. A dataset to model Levantine landcover and land-use change connected to climate change, the Arab Spring and COVID-19. Data in Brief (submitted: December 2023).

The Data in Brief article contains the supplement information and is the related data paper to:

Kempf, M. Climate change, the Arab Spring, and COVID-19 - Impacts on landcover transformations in the Levant. Journal of Arid Environments (revision submitted: December 2023).

Description/abstract

The Levant region is highly vulnerable to climate change, experiencing prolonged heat waves that have led to societal crises and population displacement. Since 2010, the area has been marked by socio-political turmoil, including the Syrian civil war and currently the escalation of the so-called Israeli-Palestinian Conflict, which strained neighbouring countries like Jordan due to the influx of Syrian refugees and increases population vulnerability to governmental decision-making. Jordan, in particular, has seen rapid population growth and significant changes in land-use and infrastructure, leading to over-exploitation of the landscape through irrigation and construction. This dataset uses climate data, satellite imagery, and land cover information to illustrate the substantial increase in construction activity and highlights the intricate relationship between climate change predictions and current socio-political developments in the Levant.

Folder structure

The main folder after download contains all data, in which the following subfolders are stored are stored as zipped files:

“code” stores the above described 9 code chunks to read, extract, process, analyse, and visualize the data.

“MODIS_merged” contains the 16-days, 250 m resolution NDVI imagery merged from three tiles (h20v05, h21v05, h21v06) and cropped to the study area, n=510, covering January 2001 to December 2022 and including January and February 2023.

“mask” contains a single shapefile, which is the merged product of administrative boundaries, including Jordan, Lebanon, Israel, Syria, and Palestine (“MERGED_LEVANT.shp”).

“yield_productivity” contains .csv files of yield information for all countries listed above.

“population” contains two files with the same name but different format. The .csv file is for processing and plotting in R. The .ods file is for enhanced visualization of population dynamics in the Levant (Socio_cultural_political_development_database_FAO2023.ods).

“GLDAS” stores the raw data of the NASA Global Land Data Assimilation System datasets that can be read, extracted (variable name), and processed using code “8_GLDAS_read_extract_trend” from the respective folder. One folder contains data from 1975-2022 and a second the additional January and February 2023 data.

“built_up” contains the landcover and built-up change data from 1975 to 2022. This folder is subdivided into two subfolder which contain the raw data and the already processed data. “raw_data” contains the unprocessed datasets and “derived_data” stores the cropped built_up datasets at 5 year intervals, e.g., “Levant_built_up_1975.tif”.

Code structure

1_MODIS_NDVI_hdf_file_extraction.R

This is the first code chunk that refers to the extraction of MODIS data from .hdf file format. The following packages must be installed and the raw data must be downloaded using a simple mass downloader, e.g., from google chrome. Packages: terra. Download MODIS data from after registration from: https://lpdaac.usgs.gov/products/mod13q1v061/ or https://search.earthdata.nasa.gov/search (MODIS/Terra Vegetation Indices 16-Day L3 Global 250m SIN Grid V061, last accessed, 09th of October 2023). The code reads a list of files, extracts the NDVI, and saves each file to a single .tif-file with the indication “NDVI”. Because the study area is quite large, we have to load three different (spatially) time series and merge them later. Note that the time series are temporally consistent.

2_MERGE_MODIS_tiles.R

In this code, we load and merge the three different stacks to produce large and consistent time series of NDVI imagery across the study area. We further use the package gtools to load the files in (1, 2, 3, 4, 5, 6, etc.). Here, we have three stacks from which we merge the first two (stack 1, stack 2) and store them. We then merge this stack with stack 3. We produce single files named NDVI_final_*consecutivenumber*.tif. Before saving the final output of single merged files, create a folder called “merged” and set the working directory to this folder, e.g., setwd("your directory_MODIS/merged").

3_CROP_MODIS_merged_tiles.R

Now we want to crop the derived MODIS tiles to our study area. We are using a mask, which is provided as .shp file in the repository, named "MERGED_LEVANT.shp". We load the merged .tif files and crop the stack with the vector. Saving to individual files, we name them “NDVI_merged_clip_*consecutivenumber*.tif. We now produced single cropped NDVI time series data from MODIS.
The repository provides the already clipped and merged NDVI datasets.

4_TREND_analysis_NDVI.R

Now, we want to perform trend analysis from the derived data. The data we load is tricky as it contains 16-days return period across a year for the period of 22 years. Growing season sums contain MAM (March-May), JJA (June-August), and SON (September-November). December is represented as a single file, which means that the period DJF (December-February) is represented by 5 images instead of 6. For the last DJF period (December 2022), the data from January and February 2023 can be added. The code selects the respective images from the stack, depending on which period is under consideration. From these stacks, individual annually resolved growing season sums are generated and the slope is calculated. We can then extract the p-values of the trend and characterize all values with high confidence level (0.05). Using the ggplot2 package and the melt function from reshape2 package, we can create a plot of the reclassified NDVI trends together with a local smoother (LOESS) of value 0.3.
To increase comparability and understand the amplitude of the trends, z-scores were calculated and plotted, which show the deviation of the values from the mean. This has been done for the NDVI values as well as the GLDAS climate variables as a normalization technique.

5_BUILT_UP_change_raster.R

Let us look at the landcover changes now. We are working with the terra package and get raster data from here: https://ghsl.jrc.ec.europa.eu/download.php?ds=bu (last accessed 03. March 2023, 100 m resolution, global coverage). Here, one can download the temporal coverage that is aimed for and reclassify it using the code after cropping to the individual study area. Here, I summed up different raster to characterize the built-up change in continuous values between 1975 and 2022.

6_POPULATION_numbers_plot.R

For this plot, one needs to load the .csv-file “Socio_cultural_political_development_database_FAO2023.csv” from the repository. The ggplot script provided produces the desired plot with all countries under consideration.

7_YIELD_plot.R

In this section, we are using the country productivity from the supplement in the repository “yield_productivity” (e.g., "Jordan_yield.csv". Each of the single country yield datasets is plotted in a ggplot and combined using the patchwork package in R.

8_GLDAS_read_extract_trend

The last code provides the basis for the trend analysis of the climate variables used in the paper. The raw data can be accessed https://disc.gsfc.nasa.gov/datasets?keywords=GLDAS%20Noah%20Land%20Surface%20Model%20L4%20monthly&page=1 (last accessed 9th of October 2023). The raw data comes in .nc file format and various variables can be extracted using the [“^a variable name”] command from the spatraster collection. Each time you run the code, this variable name must be adjusted to meet the requirements for the variables (see this link for abbreviations: https://disc.gsfc.nasa.gov/datasets/GLDAS_CLSM025_D_2.0/summary, last accessed 09th of October 2023; or the respective code chunk when reading a .nc file with the ncdf4 package in R) or run print(nc) from the code or use names(the spatraster collection).
Choosing one variable, the code uses the MERGED_LEVANT.shp mask from the repository to crop and mask the data to the outline of the study area.
From the processed data, trend analysis are conducted and z-scores were calculated following the code described above. However, annual trends require the frequency of the time series analysis to be set to value = 12. Regarding, e.g., rainfall, which is measured as annual sums and not means, the chunk r.sum=r.sum/12 has to be removed or set to r.sum=r.sum/1 to avoid calculating annual mean values (see other variables). Seasonal subset can be calculated as described in the code. Here, 3-month subsets were chosen for growing seasons, e.g. March-May (MAM), June-July (JJA), September-November (SON), and DJF (December-February, including Jan/Feb of the consecutive year).
From the data, mean values of 48 consecutive years are calculated and trend analysis are performed as describe above. In the same way, p-values are extracted and 95 % confidence level values are marked with dots on the raster plot. This analysis can be performed with a much longer time series, other variables, ad different spatial extent across the globe due to the availability of the GLDAS variables.

This archive contains code and data for reproducing the analysis for “Replication Data for Revisiting ‘The Rise and Decline’ in a Population of Peer Production Projects”. Depending on what you hope to do with the data you probabbly do not want to download all of the files. Depending on your computation resources you may not be able to run all stages of the analysis. The code for all stages of the analysis, including typesetting the manuscript and running the analysis, is in code.tar. If you only want to run the final analysis or to play with datasets used in the analysis of the paper, you want intermediate_data.7z or the uncompressed tab and csv files. The data files are created in a four-stage process. The first stage uses the program “wikiq” to parse mediawiki xml dumps and create tsv files that have edit data for each wiki. The second stage generates all.edits.RDS file which combines these tsvs into a dataset of edits from all the wikis. This file is expensive to generate and at 1.5GB is pretty big. The third stage builds smaller intermediate files that contain the analytical variables from these tsv files. The fourth stage uses the intermediate files to generate smaller RDS files that contain the results. Finally, knitr and latex typeset the manuscript. A stage will only run if the outputs from the previous stages do not exist. So if the intermediate files exist they will not be regenerated. Only the final analysis will run. The exception is that stage 4, fitting models and generating plots, always runs. If you only want to replicate from the second stage onward, you want wikiq_tsvs.7z. If you want to replicate everything, you want wikia_mediawiki_xml_dumps.7z.001 wikia_mediawiki_xml_dumps.7z.002, and wikia_mediawiki_xml_dumps.7z.003. These instructions work backwards from building the manuscript using knitr, loading the datasets, running the analysis, to building the intermediate datasets. Building the manuscript using knitr This requires working latex, latexmk, and knitr installations. Depending on your operating system you might install these packages in different ways. On Debian Linux you can run apt install r-cran-knitr latexmk texlive-latex-extra. Alternatively, you can upload the necessary files to a project on Overleaf.com. Download code.tar. This has everything you need to typeset the manuscript. Unpack the tar archive. On a unix system this can be done by running tar xf code.tar. Navigate to code/paper_source. Install R dependencies. In R. run install.packages(c("data.table","scales","ggplot2","lubridate","texreg")) On a unix system you should be able to run make to build the manuscript generalizable_wiki.pdf. Otherwise you should try uploading all of the files (including the tables, figure, and knitr folders) to a new project on Overleaf.com. Loading intermediate datasets The intermediate datasets are found in the intermediate_data.7z archive. They can be extracted on a unix system using the command 7z x intermediate_data.7z. The files are 95MB uncompressed. These are RDS (R data set) files and can be loaded in R using the readRDS. For example newcomer.ds <- readRDS("newcomers.RDS"). If you wish to work with these datasets using a tool other than R, you might prefer to work with the .tab files. Running the analysis Fitting the models may not work on machines with less than 32GB of RAM. If you have trouble, you may find the functions in lib-01-sample-datasets.R useful to create stratified samples of data for fitting models. See line 89 of 02_model_newcomer_survival.R for an example. Download code.tar and intermediate_data.7z to your working folder and extract both archives. On a unix system this can be done with the command tar xf code.tar && 7z x intermediate_data.7z. Install R dependencies. install.packages(c("data.table","ggplot2","urltools","texreg","optimx","lme4","bootstrap","scales","effects","lubridate","devtools","roxygen2")). On a unix system you can simply run regen.all.sh to fit the models, build the plots and create the RDS files. Generating datasets Building the intermediate files The intermediate files are generated from all.edits.RDS. This process requires about 20GB of memory. Download all.edits.RDS, userroles_data.7z,selected.wikis.csv, and code.tar. Unpack code.tar and userroles_data.7z. On a unix system this can be done using tar xf code.tar && 7z x userroles_data.7z. Install R dependencies. In R run install.packages(c("data.table","ggplot2","urltools","texreg","optimx","lme4","bootstrap","scales","effects","lubridate","devtools","roxygen2")). Run 01_build_datasets.R. Building all.edits.RDS The intermediate RDS files used in the analysis are created from all.edits.RDS. To replicate building all.edits.RDS, you only need to run 01_build_datasets.R when the int... Visit https://dataone.org/datasets/sha256%3Acfa4980c107154267d8eb6dc0753ed0fde655a73a062c0c2f5af33f237da3437 for complete metadata about this dataset.

File List breedingBirdData.txt butterflyData.txt ExampleSession.txt MultiSpeciesSiteOcc.R MultiSpeciesSiteOccModel.txt CumNumSpeciesPresent.R

Description “breedingBirdData.txt” is an example data set in ASCII comma-delimited format. Each row corresponds to data for a single species observed in the avian survey. The 50 columns correspond to 50 sample locations. “butterflyData.txt” is an example data set in ASCII comma-delimited format. Each row corresponds to data for a single species observed in the butterfly survey. The 20 columns correspond to 20 sample locations. “ExampleSession.txt” illustrates an example session in R where the butterfly data are read into memory and then analyzed using the R and WinBUGS code. “MultiSpeciesSiteOcc.R” defines an R function for fitting the model of species occurrence and detection to data. This function specifies a Gibbs sampler wherein 55000 random draws are computed for each of 4 different Markov chains. These computations may require nontrivial execution times. For example, analysis of the avian data required about 4 hours using a computer equipped with a 3.20 GHz Pentium 4 processor. Analysis of the butterfly data required about 1.5 hours. “MultiSpeciesSiteOccModel.txt” contains WinBUGS code for specifying the model of species occurrence and detection. “CumNumSpeciesPresent.R” defines an R function for computing a sample of the posterior-predictive distribution of a species-accumulation curve whose abscissa ranges from 1 to nsites sites.

This dataset contains files reconstructing single-cell data presented in 'Reference transcriptomics of porcine peripheral immune cells created through bulk and single-cell RNA sequencing' by Herrera-Uribe & Wiarda et al. 2021. Samples of peripheral blood mononuclear cells (PBMCs) were collected from seven pigs and processed for single-cell RNA sequencing (scRNA-seq) in order to provide a reference annotation of porcine immune cell transcriptomics at enhanced, single-cell resolution. Analysis of single-cell data allowed identification of 36 cell clusters that were further classified into 13 cell types, including monocytes, dendritic cells, B cells, antibody-secreting cells, numerous populations of T cells, NK cells, and erythrocytes. Files may be used to reconstruct the data as presented in the manuscript, allowing for individual query by other users. Scripts for original data analysis are available at https://github.com/USDA-FSEPRU/PorcinePBMCs_bulkRNAseq_scRNAseq. Raw data are available at https://www.ebi.ac.uk/ena/browser/view/PRJEB43826. Funding for this dataset was also provided by NRSP8: National Animal Genome Research Program (https://www.nimss.org/projects/view/mrp/outline/18464). Resources in this dataset:Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - All Cells 10X Format. File Name: PBMC7_AllCells.zipResource Description: Zipped folder containing PBMC counts matrix, gene names, and cell IDs. Files are as follows: matrix of gene counts* (matrix.mtx.gx) gene names (features.tsv.gz) cell IDs (barcodes.tsv.gz) *The ‘raw’ count matrix is actually gene counts obtained following ambient RNA removal. During ambient RNA removal, we specified to calculate non-integer count estimations, so most gene counts are actually non-integer values in this matrix but should still be treated as raw/unnormalized data that requires further normalization/transformation. Data can be read into R using the function Read10X().Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - All Cells Metadata. File Name: PBMC7_AllCells_meta.csvResource Description: .csv file containing metadata for cells included in the final dataset. Metadata columns include: nCount_RNA = the number of transcripts detected in a cell nFeature_RNA = the number of genes detected in a cell Loupe = cell barcodes; correspond to the cell IDs found in the .h5Seurat and 10X formatted objects for all cells prcntMito = percent mitochondrial reads in a cell Scrublet = doublet probability score assigned to a cell seurat_clusters = cluster ID assigned to a cell PaperIDs = sample ID for a cell celltypes = cell type ID assigned to a cellResource Title: Herrera-Uribe & Wiarda et al. PBMCs - All Cells PCA Coordinates. File Name: PBMC7_AllCells_PCAcoord.csvResource Description: .csv file containing first 100 PCA coordinates for cells. Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - All Cells t-SNE Coordinates. File Name: PBMC7_AllCells_tSNEcoord.csvResource Description: .csv file containing t-SNE coordinates for all cells.Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - All Cells UMAP Coordinates. File Name: PBMC7_AllCells_UMAPcoord.csvResource Description: .csv file containing UMAP coordinates for all cells.Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - CD4 T Cells t-SNE Coordinates. File Name: PBMC7_CD4only_tSNEcoord.csvResource Description: .csv file containing t-SNE coordinates for only CD4 T cells (clusters 0, 3, 4, 28). A dataset of only CD4 T cells can be re-created from the PBMC7_AllCells.h5Seurat, and t-SNE coordinates used in publication can be re-assigned using this .csv file.Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - CD4 T Cells UMAP Coordinates. File Name: PBMC7_CD4only_UMAPcoord.csvResource Description: .csv file containing UMAP coordinates for only CD4 T cells (clusters 0, 3, 4, 28). A dataset of only CD4 T cells can be re-created from the PBMC7_AllCells.h5Seurat, and UMAP coordinates used in publication can be re-assigned using this .csv file.Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - Gamma Delta T Cells UMAP Coordinates. File Name: PBMC7_GDonly_UMAPcoord.csvResource Description: .csv file containing UMAP coordinates for only gamma delta T cells (clusters 6, 21, 24, 31). A dataset of only gamma delta T cells can be re-created from the PBMC7_AllCells.h5Seurat, and UMAP coordinates used in publication can be re-assigned using this .csv file.Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - Gamma Delta T Cells t-SNE Coordinates. File Name: PBMC7_GDonly_tSNEcoord.csvResource Description: .csv file containing t-SNE coordinates for only gamma delta T cells (clusters 6, 21, 24, 31). A dataset of only gamma delta T cells can be re-created from the PBMC7_AllCells.h5Seurat, and t-SNE coordinates used in publication can be re-assigned using this .csv file.Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - Gene Annotation Information. File Name: UnfilteredGeneInfo.txtResource Description: .txt file containing gene nomenclature information used to assign gene names in the dataset. 'Name' column corresponds to the name assigned to a feature in the dataset.Resource Title: Herrera-Uribe & Wiarda et al. PBMCs - All Cells H5Seurat. File Name: PBMC7.tarResource Description: .h5Seurat object of all cells in PBMC dataset. File needs to be untarred, then read into R using function LoadH5Seurat().

Dataset

This dataset was created by Medou Neine

Contents

The dataset contains fiber R object, stored as an RDS file for use in R package for analysis of long-read sequencing.

Ecological processes and biodiversity patterns are strongly affected by how animals move through the landscape. However, it remains challenging to predict animal movement and space use. Here we present our new R package enerscape to quantify and predict animal movement in real landscapes based on energy expenditure.

Enerscape integrates a general locomotory model for terrestrial animals with GIS tools in order to map energy costs of movement in a given environment, resulting in energy landscapes that reflect how energy expenditures may shape habitat use. Enerscape only requires topographic data (elevation) and the body mass of the studied animal. To illustrate the potential of enerscape, we analyze the energy landscape for the Marsican bear (Ursus arctos marsicanus) in a protected area in central Italy in order to identify least-cost paths and high-connectivity areas with low energy costs of travel.


Enerscape allowed us to identify travel routes for the bear that minimize...

R script used for processing the 16S Illumina paired end read data using the DADA2 pipeline.

For any questions about this data please email me at jacob@crimedatatool.com. If you use this data, please cite it.Version 3 release notes:Adds data in the following formats: Excel.Changes project name to avoid confusing this data for the ones done by NACJD.Version 2 release notes:Adds data for 2017.Adds a "number_of_months_reported" variable which says how many months of the year the agency reported data.Property Stolen and Recovered is a Uniform Crime Reporting (UCR) Program data set with information on the number of offenses (crimes included are murder, rape, robbery, burglary, theft/larceny, and motor vehicle theft), the value of the offense, and subcategories of the offense (e.g. for robbery it is broken down into subcategories including highway robbery, bank robbery, gas station robbery). The majority of the data relates to theft. Theft is divided into subcategories of theft such as shoplifting, theft of bicycle, theft from building, and purse snatching. For a number of items stolen (e.g. money, jewelry and previous metals, guns), the value of property stolen and and the value for property recovered is provided. This data set is also referred to as the Supplement to Return A (Offenses Known and Reported). All the data was received directly from the FBI as text or .DTA files. I created a setup file based on the documentation provided by the FBI and read the data into R using the package asciiSetupReader. All work to clean the data and save it in various file formats was also done in R. For the R code used to clean this data, see here: https://github.com/jacobkap/crime_data. The Word document file available for download is the guidebook the FBI provided with the raw data which I used to create the setup file to read in data.There may be inaccuracies in the data, particularly in the group of columns starting with "auto." To reduce (but certainly not eliminate) data errors, I replaced the following values with NA for the group of columns beginning with "offenses" or "auto" as they are common data entry error values (e.g. are larger than the agency's population, are much larger than other crimes or months in same agency): 1000, 2000, 3000, 4000, 5000, 6000, 7000, 8000, 9000, 10000, 20000, 30000, 40000, 50000, 60000, 70000, 80000, 90000, 100000, 99942. This cleaning was NOT done on the columns starting with "value."For every numeric column I replaced negative indicator values (e.g. "j" for -1) with the negative number they are supposed to be. These negative number indicators are not included in the FBI's codebook for this data but are present in the data. I used the values in the FBI's codebook for the Offenses Known and Clearances by Arrest data.To make it easier to merge with other data, I merged this data with the Law Enforcement Agency Identifiers Crosswalk (LEAIC) data. The data from the LEAIC add FIPS (state, county, and place) and agency type/subtype. If an agency has used a different FIPS code in the past, check to make sure the FIPS code is the same as in this data.

Cover crops provide many agroecosystem services, including weed suppression, which is partially exerted through release of allelopathic benzoxazinoid (BX) compounds. This research characterizes (1) changes in concentrations of BX compounds in shoots, roots, and soil at three growth stages (GS) of cereal rye (Secale cereale L.), and (2) their degradation over time following termination. Concentrations of shoot dominant BX compounds, DIBOA-glc and DIBOA, were least at GS 83 (boot). The root dominant BX compound, HMBOA-glc, concentration was least at GS 54 (elongation). Rhizosphere soil BX concentrations were 1000 times smaller than in root tissues. Dominant compounds in soil were HMBOA-glc and HMBOA. Concentrations of BX compounds were similar for soil near root crowns and between-rows. Soil BX concentrations following cereal rye termination declined exponentially over time in three of four treatments: incorporated shoots (S) and roots (R), no-till S+R (cereal rye rolled flat), and no-till R (shoots removed), but not in no-till S. On the day following cereal rye termination, soil concentrations of HMBOA-glc and HMBOA in these three treatments increased above initial concentrations. Concentrations of these two compounds decreased the fastest while DIBOA-glc declined the slowest (half-life of 4 d in no-till S+R soil). Placement of shoots on the surface of an area where cereal rye had not grown (no-till S) did not increase soil concentrations of BX compounds. The short duration and complex dynamics of BX compounds in soil prior to and following termination illustrate the limited window for enhancing weed suppression by cereal rye allelochemicals; valuable information for programs breeding for enhanced weed suppression. In addition to the data analyzed for this article, we also include the R code. Resources in this dataset:Resource Title: BX data following termination. File Name: FinalBXsForMatt-20200908.csvResource Description: For each sample, gives the time, depth, location, and plot treatment, and then the compound concentrations. This is the principal data set analyzed with the R (anal2-cleaned.r) code, see that code for use.Resource Title: BX compounds from 3rd sampling time before termination. File Name: soil2-20201123.csvResource Description: These data are for comparison with the post termination data. They were taken at the 3rd sampling time (pre-termination), a day prior to termination. Each sample is identified with a treatment, date, and plot location, in addition to the BX concentrations. See R code (anal2-cleaned.r) for how this file is used.Resource Title: Soil location (within row versus between row) values of BX compounds. File Name: s2b.csvResource Description: Each row gives the average BX compound for each soil location (within row versus between row) for the second sample for each plot. These data are combined with bx3 (the data set read in from the file , "FinalBXsForMatt-20200908.csv"). See R (anal2-cleaned.r) code for use.Resource Title: R code for analysis of the decay (post-termination) BX data.. File Name: anal2-cleaned.rResource Description: This is the R code used to analyze the termination data. It also creates and writes out some data subsets (used for analysis and plots) that are later read in.Resource Software Recommended: R version 3.6.3,url: https://www.R-project.org/ Resource Title: Tissue BX compounds. File Name: tissues20210728b.csvResource Description: Data file holding results from a tissue analysis for BX compounds, in ug, from shoots and roots, and at various sampling times. Read into the R file, anal1-cleaned.r where it is used in a statistical analysis and to create figures.Resource Title: BX compounds from soil with a live rye cover crop. File Name: soil2-20201214.csvResource Description: BX compounds (in ng/g dry wt), by treatment, sampling time, date, and plot ID. These are data are read into the R program, anal1-cleaned.r, for analysis and to create figures. These are soil samples taken from locations with a live rye plant cover crop.Resource Title: R code for BX analyses of soil under rye and plant tissues. File Name: anal1-cleaned.rResource Description: R code for analysis of the soil BX compounds under a live rye cover crop at different growing stages, and for the analysis of tissue BX compounds. In addition to statistical analyses, code in this file creates figures, also some statistical output that is used to create a file that is later read in for figure creation (s2-CLD20220730-Stage.csv).Resource Software Recommended: R version 3.6.3,url: https://www.R-project.org/ Resource Title: Description of data files for anal2-cleaned.r. File Name: readme2.txtResource Description: Describes the input files used in the R code in anal2-cleaned.r, including descriptions and formats for each field. The file also describes some output (results) files that were uploaded to this site. This is a plain ASCII text file.Resource Title: Estimates produced by anal2-cleaned.r from statistical modeling.. File Name: Estimates20201110.csvResource Description: Estimates produced by anal2-cleaned.r from statistical modeling (see readme2.txt)Resource Title: Summary statistics from anal2-cleaned.r. File Name: CV20210412.csvResource Description: Summary statistics from anal2-cleaned.r, used for plotsResource Title: Data summaries (same as CV20210412.csv), rescaled. File Name: RESCALE-20210412.csvResource Description: Same as "CV20210412.csv" except log of data have been rescaled to minimum at least zero and maximum one, see readme2.txtResource Title: Statistical summaries for different stages. File Name: s2-CLD20220730-Stage.csvResource Description: Statistical summaries used for creating a figure (not used in paper), used in anal1-cleaned.r; data for soil BX under living rye.Resource Title: Description of data files for anal1-cleaned.r. File Name: readme1.txtResource Description: Contains general descriptions of data imported into anal1-cleaned.r, and a description of each field. Also contains some descriptions of files output by anal1-cleaned.r, used to create tables or figures.

The R Manual for QCA entails a PDF file that describes all the steps and code needed to prepare and conduct a Qualitative Comparative Analysis (QCA) study in R. This is complemented by an R Script that can be customized as needed. The dataset further includes two files with sample data, for the set-theoretic analysis and the visualization of QCA results. The R Manual for QCA is the online appendix to "Qualitative Comparative Analysis: An Introduction to Research Design and Application", Georgetown University Press, 2021.

In this course, you will learn to work within the free and open-source R environment with a specific focus on working with and analyzing geospatial data. We will cover a wide variety of data and spatial data analytics topics, and you will learn how to code in R along the way. The Introduction module provides more background info about the course and course set up. This course is designed for someone with some prior GIS knowledge. For example, you should know the basics of working with maps, map projections, and vector and raster data. You should be able to perform common spatial analysis tasks and make map layouts. If you do not have a GIS background, we would recommend checking out the West Virginia View GIScience class. We do not assume that you have any prior experience with R or with coding. So, don't worry if you haven't developed these skill sets yet. That is a major goal in this course. Background material will be provided using code examples, videos, and presentations. We have provided assignments to offer hands-on learning opportunities. Data links for the lecture modules are provided within each module while data for the assignments are linked to the assignment buttons below. Please see the sequencing document for our suggested order in which to work through the material. After completing this course you will be able to: prepare, manipulate, query, and generally work with data in R. perform data summarization, comparisons, and statistical tests. create quality graphs, map layouts, and interactive web maps to visualize data and findings. present your research, methods, results, and code as web pages to foster reproducible research. work with spatial data in R. analyze vector and raster geospatial data to answer a question with a spatial component. make spatial models and predictions using regression and machine learning. code in the R language at an intermediate level.