Jack 'Hardcase' Harris & Ralph 'Ricky' Reynolds: The informant's dubious alibi for the night of the theft - Generated by Conversation Dataset Generator

This dataset was generated using the Conversation Dataset Generator script available at https://github.com/cahlen/conversation-dataset-generator.

  Generation Parameters

Number of Conversations Requested: 10 Number of Conversations Successfully Generated: 10 Total Turns: 106 Model ID: meta-llama/Meta-Llama-3-8B-Instruct… See the full description on the dataset page: https://huggingface.co/datasets/cahlen/cdg-noir-interrogation-ex10.

Sequences of primers used to interrogate the inversion products.

Genomics is narrowing uncertainty in the phylogenetic structure for many amniote groups. For one of the most diverse and species-rich groups, the squamate reptiles (lizards and snakes, amphisbaenians), an inverse correlation between the number of taxa and loci sampled still persists across all publications using DNA sequence data and reaching a consensus on the relationships among them has been highly problematic. Here, we use high-throughput sequence data from 289 samples covering 75 families of squamates to address phylogenetic affinities, estimate divergence times, and characterize residual topological uncertainty in the presence of genome scale data. Importantly, we address genomic support for the traditional taxonomic groupings Scleroglossa and Macrostomata using novel machine-learning techniques. We interrogate genes using various metrics inherent to these loci, including parsimony-informative sites, phylogenetic informativeness, length, gaps, number of substitutions, and site con...

The General Order detailing RPD's interrogation procedures policy.

This dataset is about book subjects. It has 2 rows and is filtered where the books is The interrogation of Joan of Arc. It features 10 columns including number of authors, number of books, earliest publication date, and latest publication date.

Data for D. A. Long, B. J. Reschovsky, F. Zhou, Y. Bao, T. W. LeBrun, and J. J. Gorman, "Electro-optic frequency combs for rapid interrogation in cavity optomechanics," Optics Letters Vol. 46, Issue 3, pp. 645-648 (2021), https://doi.org/10.1364/OL.405299

The effects of working circumstances and intended uses on the transcripts of police interrogations cannot be underestimated. In the Netherlands, police transcripts are usually drawn up in the course of the interrogation by the interrogator or, when two police officers conduct the interrogation, by the reporting officer. Contemporaneous transcription involves the interrogators in a complex configuration of interactional commitments. They have to find a way to coordinate the talk and the typing, they must transcribe the talk of an event they themselves participate in, they must do justice to the suspects' story while also taking into account the intended readership of the police report, and they must produce a document that can serve as an official piece of evidence in the criminal case. In studying recorded police interrogations and their transcripts I realised that my own transcripts are also related to their intended uses and to my working circumstances. My transcriptions are much more detailed than those of the police, which draws the attention to the differences between them. The most noticeable difference is that police transcripts focus on substance and mine on interaction. Police transcripts are meant to be evidence of the offence and mine of the talk. But there are also similarities. Both police transcripts and those of mine are selective. Police transcripts orient to their relevance for building a case, mine orient to their relevance for my research questions. Both police transcripts and those of mine treat the transcript as the talk it is meant to represent. For a criminal case this means that in court suspects are held accountable for what the police wrote down as their statement, which disregards the fact that the police transcript is a coproduction.

Genetic Analysis to interrogate tumor heterogeneity in lung adenocarcinoma

This dataset contains inter-receiver Love wave traveltimes among 61 seismometers around the British Isles. The data was originally obtained by cross-correlation of ambient noise data recorded by the 61 receivers during 2001 to 2003, 2006 to 2007 and in 2010. More details can be found in Transdimensional Love-wave tomography of the British Isles and shear-velocity structure of the East Irish Sea Basin from ambient-noise interferometry (https://doi.org/10.1093/gji/ggw286) and Interrogating Subsurface Structures using Probabilistic Tomography: an example assessing the volume of Irish Sea basins (https://doi.org/10.1029/2022JB024098). The dataset relates to the manuscript 'Bayesian Inversion, Uncertainty Analysis, and Interrogation using Boosting Variational Inference'.

The superior performance of a multiple NP workflow was shown compared to high-complexity, state-of-the-art, high-pH fractionation strategies in terms of depth and precision. We also evaluate the link between the physicochemical properties of NPs and their differential protein selectivity.

Phylogenomic studies using genome†wide datasets are quickly becoming the state of the art for systematics and comparative studies, but in many cases, they result in strongly supported incongruent results. The extent to which this conflict is real depends on different sources of error potentially affecting big datasets (assembly, stochastic, and systematic error). Here, we apply a recently developed methodology (GGI or gene genealogy interrogation) and data curation to new and published datasets with more than 1000 exons, 500 ultraconserved element (UCE) loci, and transcriptomic sequences that support incongruent hypotheses. The contentious non†monophyly of the order Characiformes proposed by two studies is shown to be a spurious outcome induced by sample contamination in the transcriptomic dataset and an ambiguous result due to poor taxonomic sampling in the UCE dataset. By exploring the effects of number of taxa and loci used for analysis, we show that the power of GGI to discriminate ...

This dataset is about books. It has 4 rows and is filtered where the book subjects is Military interrogation-United States. It features 9 columns including author, publication date, language, and book publisher.

In two experiments, we tested the hypothesis that guilt feelings would elevate the probability of making a false confession. In Experiment 1 (N = 146), a confederate induced guilt feelings by asking participants to cheat on a task. The experimenter then falsely accused participants of having pressed a forbidden key, causing a computer crash. In Experiment 2 (N = 108), a confederate was punished every time participants could not answer a quiz question. The confederate later cheated in a game and asked participants to take the blame. In Experiment 1, 100 participants (68.5%) falsely confessed to pressing the key. In Experiment 2, 39 participants (36.1%) falsely confessed to cheating. Guilt manipulations had no effect on false confession rates. When exploring the effect of guilt feelings, five of eight tests were statistically non-significant. As yet, there is insufficient evidence to argue that guilt feelings are a major determinant of false confessions.

"High dynamic range electro-optic dual-comb interrogation of optomechanical sensors" to be published in Optics Letters.

Protein-Protein, Genetic, and Chemical Interactions for Zhang D (2021):Ultra-fast and onsite interrogation of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in waters via surface enhanced Raman scattering (SERS). curated by BioGRID (https://thebiogrid.org); ABSTRACT: The outbreak of coronavirus infectious disease-2019 (COVID-19) pneumonia challenges the rapid interrogation of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in human and environmental samples. In this study, we developed an assay using surface enhanced Raman scattering (SERS) coupled with multivariate analysis to detect SARS-CoV-2 in an ultra-fast manner without any pretreatment (e.g., RNA extraction). Using silver-nanorod SERS array functionalized with cellular receptor angiotensin-converting enzyme 2 (ACE2), we obtained strong SERS signals of ACE2 at 1032, 1051, 1089, 1189, 1447 and 1527 cm-1. The recognition and binding of receptor binding domain (RBD) of SARS-CoV-2 spike protein on SERS assay significantly quenched the spectral intensities of most peaks and exhibited a shift from 1189 to 1182 cm-1. On-site tests on 23 water samples with a portable Raman spectrometer proved its accuracy and easy-operation for spot detection of SARS-CoV-2 to evaluate disinfection performance, explore viral survival in environmental media, assess viral decay in wastewater treatment plant and track SARS-CoV-2 in pipe network. Our findings raise a state-of-the-art spectroscopic tool to screen and interrogate viruses with RBD for human cell entry, proving its feasibility and potential as an ultra-fast detection tool for wastewater-based epidemiology.

We applied RNA-protein interactome capture method called XRNAX to shed light on RNA-bound proteome in the brain affected with dysmyelination. We found that sets of canonical RBPs that are known to regulate alternative splicing and being engaged in the formation of cytoplasmic granules are perturbed at the level of their RNA interactome in the mouse brain with the inborn deficit of myelin. This dataset contains the whole brain proteomics data as well as LC-MS/MS data from XRNAX extracts enriched in brain RBPs following UV crosslink of RNA-protein interaction in the brain tissue homogenates from wildtype C57BL6/N (experiments 1 and 2) and shiverer mouse model (experiment 3). Whole brain tissues were homogenized using two different methods: biopulverization of the flash-frozen tissue in liquid nitrogen (experiments 2 and 3), and alternatively, tissues submersed in PBS buffer immediately after dissections were disintegrated with Dounce homogenizer (experiment 1).

Behavior depends on coordinated activity across multiple brain regions. Within such networks, highly connected hub regions are assumed to disproportionately influence behavioral output, although this hypothesis has not been systematically evaluated. Previously, by mapping brain-wide expression of the activity-regulated gene c-fos, we identified a network of brain regions co-activated by fear memory. To test the hypothesis that hub regions are more important for network function, here, we simulated node deletion in silico in this behaviorally defined functional network. Removal of high degree nodes produced the greatest network disruption (e.g., reduction in global efficiency). To test these predictions in vivo, we examined the impact of post-training chemogenetic silencing of different network nodes on fear memory consolidation. In a series of independent experiments encompassing 25% of network nodes (i.e., 21/84 brain regions), we found that node degree accurately predicted observed deficits in memory consolidation, with silencing of highly connected hubs producing the largest impairments.

The breast cancer suppressor BRCA2 controls the recombinase RAD51 in the reactions that mediate homologous DNA recombination, an essential cellular process required for the error-free repair of DNA double-stranded breaks. The primary mode of interaction between BRCA2 and RAD51 is through the BRC repeats, which are ∼35 residue peptide motifs that interact directly with RAD51 in vitro. Human BRCA2, like its mammalian orthologues, contains 8 BRC repeats whose sequence and spacing are evolutionarily conserved. Despite their sequence conservation, there is evidence that the different human BRC repeats have distinct capacities to bind RAD51. A previously published crystal structure reports the structural basis of the interaction between human BRC4 and the catalytic core domain of RAD51. However, no structural information is available regarding the binding of the remaining seven BRC repeats to RAD51, nor is it known why the BRC repeats show marked variation in binding affinity to RAD51 despite only subtle sequence variation. To address these issues, we have performed fluorescence polarisation assays to indirectly measure relative binding affinity, and applied computational simulations to interrogate the behaviour of the eight human BRC-RAD51 complexes, as well as a suite of BRC cancer-associated mutations. Our computational approaches encompass a range of techniques designed to link sequence variation with binding free energy. They include MM-PBSA and thermodynamic integration, which are based on classical force fields, and a recently developed approach to computing binding free energies from large-scale quantum mechanical first principles calculations with the linear-scaling density functional code onetep. Our findings not only reveal how sequence variation in the BRC repeats directly affects affinity with RAD51 and provide significant new insights into the control of RAD51 by human BRCA2, but also exemplify a palette of computational and experimental tools for the analysis of protein-protein interactions for chemical biology and molecular therapeutics.

Fluorescent microscopy image volumes for the WormPsyQi paper.

The publication is available here: https://elifesciences.org/reviewed-preprints/91775

Full citation: Majeed, M., Han, H., Zhang, K., Cao, W. X., Liao, C. P., Hobert, O., & Lu, H. (2023). Toolkits for detailed and high-throughput interrogation of synapses in C. elegans. bioRxiv, 2023-09.

DOI: https://doi.org/10.7554/eLife.91775.1

Combined Proteomic and Transcriptomic Interrogation of the Venom Gland of Conus geographus Uncovers Novel Components and Functional Compartmentalization