3 datasets found
  1. b

    jPOSTデータベースRDF

    • dbarchive.biosciencedbc.jp
    Updated Feb 27, 2022
    + more versions
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    (2022). jPOSTデータベースRDF [Dataset]. http://doi.org/10.18908/lsdba.nbdc01594-02-004
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    Dataset updated
    Feb 27, 2022
    Description

    jPOSTデータベースをRDF形式にしたデータ。RDFスキーマは、https://rdfportal.org/dataset/jpost を参照。

  2. LC-MS (DDA) for proteomics tutorial dataset (for MS-DIAL5)

    • zenodo.org
    bin
    Updated May 9, 2025
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    Hiroshi Tsugawa; Hiroshi Tsugawa (2025). LC-MS (DDA) for proteomics tutorial dataset (for MS-DIAL5) [Dataset]. http://doi.org/10.5281/zenodo.15369007
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    binAvailable download formats
    Dataset updated
    May 9, 2025
    Dataset provided by
    Zenodohttp://zenodo.org/
    Authors
    Hiroshi Tsugawa; Hiroshi Tsugawa
    License

    Attribution 4.0 (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/
    License information was derived automatically

    Description

    LC-MS (DDA) for proteomics tutorial dataset (for MS-DIAL5)

    This dataset includes raw data (*.raw) for the LC-MS proteomics analysis tutorial of MS-DIAL5.
    The *.raw files in these data were obtained from https://repository.jpostdb.org/entry/JPST000200.
    The human_proteins_ref.fasta was obtained from https://www.uniprot.org/.

  3. Data from: Nanoscale Solid-Phase Isobaric Labeling for Multiplexed...

    • acs.figshare.com
    xlsx
    Updated Jun 4, 2023
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    Kosuke Ogata; Chia-Feng Tsai; Yasushi Ishihama (2023). Nanoscale Solid-Phase Isobaric Labeling for Multiplexed Quantitative Phosphoproteomics [Dataset]. http://doi.org/10.1021/acs.jproteome.1c00444.s002
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    xlsxAvailable download formats
    Dataset updated
    Jun 4, 2023
    Dataset provided by
    ACS Publications
    Authors
    Kosuke Ogata; Chia-Feng Tsai; Yasushi Ishihama
    License

    Attribution-NonCommercial 4.0 (CC BY-NC 4.0)https://creativecommons.org/licenses/by-nc/4.0/
    License information was derived automatically

    Description

    We established a workflow for highly sensitive multiplexed quantitative phosphoproteomics using a nanoscale solid-phase tandem mass tag (TMT) labeling reactor. Phosphopeptides were first enriched by titanium oxide chromatography and then labeled with isobaric TMT reagents in a StageTip packed with hydrophobic polymer-based sorbents. We found that TMT-labeled singly phosphorylated peptides tend to flow through the titanium oxide column. Therefore, TMT labeling should be performed after the enrichment step from tryptic peptides, resulting in the need for microscale reactions with small amounts of phosphopeptides. Using an optimized protocol for tens to hundreds of nanograms of phosphopeptides, we obtained a nearly 10-fold increase in sensitivity compared to the conventional solution-based TMT protocol. We demonstrate that this nanoscale phosphoproteomics protocol works for 50 μg of HeLa proteins treated with selumetinib, and we successfully quantified the selumetinib-regulated phosphorylated sites on a proteome scale. The MS raw data files have been deposited with the ProteomeXchange Consortium via the jPOST partner repository (https://jpostdb.org) with the data set identifier PXD025536.

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(2022). jPOSTデータベースRDF [Dataset]. http://doi.org/10.18908/lsdba.nbdc01594-02-004

jPOSTデータベースRDF

Explore at:
Dataset updated
Feb 27, 2022
Description

jPOSTデータベースをRDF形式にしたデータ。RDFスキーマは、https://rdfportal.org/dataset/jpost を参照。

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