The Language Function Analysis 2011 Corpus (LFA-11) is a German text corpus of promotional text, reviews and blog posts on music and smartphones. The texts were manually classified with respect to their topic relevance, language function, and sentiment polarity.

The purpose of the corpus is to provide textual data for the development and evaluation of approaches to language function analysis and sentiment analysis. Therefore, each text is classified by language function (personal, commercial, or informational) as well as by sentiment (positive, negative, neutral).

The corpus consists of two separated collections, which contain the texts about music and smartphones respectively. The music collection consists of 2,713 promotional texts and reviews from both users and professionals. The smartphone collection contains 2,093 blog posts on smartphones from the Spinn3r corpus.

This event has been computationally inferred from an event that has been demonstrated in another species.

The inference is based on the homology mapping from PANTHER. Briefly, reactions for which all involved PhysicalEntities (in input, output and catalyst) have a mapped orthologue/paralogue (for complexes at least 75% of components must have a mapping) are inferred to the other species. High level events are also inferred for these events to allow for easier navigation.

More details and caveats of the event inference in Reactome. For details on PANTHER see also: http://www.pantherdb.org/about.jsp

The role of Gαi proteins coupled to chemokine receptors in directed migration of immune cells is well understood. In this study we show that the separate class of Gαq/11 proteins is required for the underlying ability of T cells to migrate both randomly and in a directed chemokine-dependent manner. Interfering with Gαq or Gα11 using dominant negative cDNA constructs or siRNA for Gαq causes accumulation of LFA-1 adhesions and stalled migration. Gαq/11 has an impact on LFA-1 expression at plasma membrane level and also on its internalization. Additionally Gαq co-localizes with LFA-1- and EEA1-expressing intracellular vesicles and partially with Rap1- but not Rab11-expressing vesicles. However the influence of Gαq is not confined to the vesicles that express it, as its reduction alters intracellular trafficking of other vesicles involved in recycling. In summary vesicle-associated Gαq/11 is required for the turnover of LFA-1 adhesion that is necessary for migration. These G proteins participate directly in the initial phase of recycling and this has an impact on later stages of the endo-exocytic pathway.

Sample ID: LFA_Rd6t08_A11

X-ray tomography (CT) image data of a Ti6Al4V disc. The 3D image was generated with an X-ray tomography scan performed by Dr Llion Evans with Swansea University, Advanced Imaging of Materials (AIM) equipment.

The dataset includes: raw radiographs; scan & reconstruction parameter settings file; reconstructed 3D volume. To visualise the 3D volume use software such as ImageJ (https://imagej.net/Fiji/Downloads). The volume image data (.raw file) is in binary format and has the following characteristics: 1920 x 1920 x 1536; 16-bit; little-endian byte order.

This data is part of a 'virtual testing' benchmark study, where samples are tested physically in the lab and their microscale accurate digital equivalent are tested virtually through simulation. The technique of converting 3D volumetric images directly into finite element method (FEM) meshes is part of the Image-Based Simulation (IBSim) approach.

This data is part of a batch of samples for thermal testing via laser flash analysis (LFA), following the standards ASTM E1461 / ASTM E2585. As part of the study, controlled defects were introduced into the samples. This was achieved by machining a disc shaped recess (of defined diameter and depth) into one disc which is bonded onto another disc, so that the defect is located internally within the final sample.

The samples in the batch are named as follows.

Sample ID Diameter Thickness Recess d Recess t
LFA_RNA_### 12.6 2.5 N/A N/A
LFA_Rd0t00_### 12.6 1.25 x 2 N/A N/A
LFA_Rd8t02_### 12.6 1.25 x 2 8.0 0.2
LFA_Rd8t08_### 12.6 1.25 x 2 8.0 0.8
LFA_Rd6t02_### 12.6 1.25 x 2 6.0 0.2
LFA_Rd6t08_### 12.6 1.25 x 2 6.0 0.8
LFA_Rd4t02_### 12.6 1.25 x 2 4.0 0.2
LFA_Rd4t08_### 12.6 1.25 x 2 4.0 0.8

Where ### denotes the furnace bonding batch (A-C and N for no bonding cycle) and sample number (01-30). Values in mm.

LFA-1 (CD11a-CD18) - BioCentury Target Profiles for the biopharma industry

integrin subunit alpha L Enables ICAM-3 receptor activity and cell adhesion molecule binding activity. Involved in several processes, including T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules; and memory T cell extravasation. Located in cell surface. Part of integrin alphaL-beta2 complex. ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

The Bordetella adenylate cyclase toxinhemolysin (CyaA) and the α-hemolysin (HlyA) of Escherichia coli both belong to the family of cytolytic pore-forming Repeats in ToXin (RTX) cytotoxins. HlyA preferentially binds the αLβ2 integrin LFA-1 (CD11a/CD18) of leukocytes and can promiscuously bind and permeabilize also a variety of other cells. CyaA bears an Nterminal adenylyl cyclase enzyme (AC) domain linked to a pore-forming RTX cytolysin (Hly) moiety and binds the complement receptor 3 (CR3, αMβ2, CD11b/CD18 or Mac-1) of myeloid phagocytes, penetrates their plasma membrane and delivers into cytosol the AC enzyme. We constructed a set of CyaA/HlyA chimeras and show that the CyaC-acylated segment and the CR3-binding RTX domain of CyaA can be functionally replaced by the much shorter HlyCacylated and LFA-1-binding RTX moiety of HlyA. Instead of binding CR3, a CyaA1710/HlyA411-1024 chimera bound the LFA-1 receptor and effectively delivered the AC enzyme into Jurkat lymphoblastoma T cells. At high chimera concentrations (25 nM), the interaction with LFA-1 was not required for CyaA1-710/HlyA411-1024 binding to CHO cells. However, interaction with the LFA-1 receptor strongly enhanced the specific capacity of the bound CyaA1-710/HlyA411-1024 chimera to penetrate cells and deliver the AC enzyme into their cytosol. Hence, interaction of the acylated RTX moiety of HlyA with LFA-1 promoted a productive membrane interaction of the chimera. These results allow to delimit the residues 400 to 710 of CyaA as the 'AC translocon' sufficient for translocation of the AC enzyme polypeptide across the plasma membrane of target cells

Polymorphonuclear leukocyte (PMN) recruitment to vascular endothelium during acute inflammation involves cooperation between selectins, G-proteins, and beta2-integrins. LFA-1 (CD11a/CD18) affinity correlates with specific adhesion functions because a shift from low to intermediate affinity supports rolling on ICAM-1, whereas high affinity is associated with shear-resistant leukocyte arrest. We imaged PMN adhesion on cytokine-inflamed endothelium in a parallel-plate flow chamber to define the dynamics of beta2-integrin function during recruitment and transmigration. After arrest on inflamed endothelium, high-affinity LFA-1 aligned along the uropod-pseudopod major axis, which was essential for efficient neutrophil polarization and subsequent transmigration. An allosteric small molecule inhibitor targeted to the I-domain stabilized LFA-1 in an intermediate-affinity conformation, which supported neutrophil rolling but inhibited cell polarization and abrogated transmigration. We conclude that a shift in LFA-1 from intermediate to high affinity during the transition from rolling to arrest provides the contact-mediated signaling and guidance necessary for PMN transmigration on inflamed endothelium.

Egg development analysis data during the 2020 lobster fishing season in LFA22. Monitoring the development of lobster eggs is included in the project Deployment of a multiparametric decision support tool for the opening date of the lobster fishery for Lobster Fishing Area 22 of the Rassemblement des pêcheurs et pêcheuses des côtes des îles(RPPCI), funded by the Quebec Fisheries Fund (MAPAQ and DFO), over 2 years. Lobster eggs are taken from female eggs by two volunteer commercial fishermen who are part of the RPPCI. Eggs are harvested from 10 females once a week on each facade of the Islands (North and South). Fishermen also installed Minilog II temperature loggers supplied by Merinov on one of their cages. The analysis is done by Merinov using a binocular and Image Pro image analyzer software. The proportion of the eye to the egg, linked to the temperature data, makes it possible to estimate the hatching date of the eggs. In 2020, the commercial fishing dates were from 9 May to 11 July. Due to the Covid 19 pandemic, data for the first week could not be collected. You can find the other project data in the catalog. Environmental monitoring data are available here (https://catalogue.ogsl.ca/en/dataset/ca-cioos_96bf3c76-a010-4637-bff5-59256f2637cc) and data on lobster monitoring in preseason fisheries can be viewed here (https://catalogue.ogsl.ca/en/dataset/ca-cioos_9c10259d-9433-4be2-abf9-7eb8b5fac5ad). Données d'analyse du développement des œufs pendant la saison de pêche au homard 2020 dans la ZPH22. Le suivi du développement des oeufs de homard s'inclut dans le projet Déploiement d'un outil multiparamétrique d'aide à la décision pour la date d'ouverture de la pêche au homard pour la Zone de Pêche au Homard 22 (ZPH22) du Rassemblement des pêcheurs et pêcheuses des côtes des Îles (RPPCI), financées par le Fonds des pêches du Québec (MAPAQ et MPO), sur 2 ans. Les œufs de homards sont prélevés sur les femelles œuvées par deux pêcheurs commerciaux volontaires faisant partie du RPPCI. Les œufs sont récoltés sur 10 femelles une fois par semaine et ce sur chaque façade des îles (Nord et Sud). Les pêcheurs ont également installé, sur une de leurs cages, des enregistreurs de température de type Minilog II fournis par Merinov. L'analyse est effectuée par Merinov à l'aide d'un binoculaire et du logiciel d'analyse d'images : Image Pro. La proportion de l'œil par rapport à l'œuf, lié aux données de températures permet d'estimer la date d'éclosion des œufs. En 2020 les dates de pêche commerciale étaient du 9 mai au 11 juillet. En raison de la pandémie de la Covid 19, les données de la première semaine n'ont pas pu être recueillies. Vous pouvez retrouver les autres données du projet dans le catalogue. Les données de suivi environnemental sont disponibles ici (https://catalogue.ogsl.ca/dataset/ca-cioos_96bf3c76-a010-4637-bff5-59256f2637cc) et les données sur le suivi des homards en pêche de présaison sont visibles ici (https://catalogue.ogsl.ca/fr/dataset/ca-cioos_9c10259d-9433-4be2-abf9-7eb8b5fac5ad).

Leukotoxin (LtxA) (trade name, Leukothera) is a protein secreted by the oral bacterium Aggregatibacter actinomycetemcomitans A. actinomycetemcomitans is an oral pathogen strongly associated with development of localized aggressive periodontitis. LtxA acts as a virulence factor for A. actinomycetemcomitans by binding to the ?2 integrin lymphocyte function-associated antigen-1 (LFA-1; CD11a/CD18) on white blood cells (WBCs) and causing cell death. In addition, because of its specificity for malignant and activated WBCs, LtxA is being investigated as a therapeutic agent for treatment of hematological malignancies and autoimmune diseases. Here, we report the successful generation and characterization of Jurkat T lymphocytes with deletions in CD18, CD11a, and Fas that were engineered using CRISPR/Cas9 gene editing. Using these clones, we demonstrate the specificity of LtxA for cells expressing LFA-1. We also demonstrate the requirement of the cell death receptor Fas for LtxA-mediated cell death in T lymphocytes. We show that LFA-1 and Fas are early events in the LtxA-mediated cell death cascade as caspase activation and mitochondrial perturbation do not occur in the absence of either receptor. To our knowledge, LtxA is the first molecule, other than FasL, known to require the Fas death receptor to initiate cell death. Knowledge of the mechanism of cell death induced by LtxA will facilitate the understanding of LtxA as a bacterial virulence factor and development of it as a potential therapeutic agent.