- Z
The analysis of composition and abundance of the raft proteome of microglia...
- data.niaid.nih.gov
- explore.openaire.eu
Updated Jul 18, 2024
Share
Facebook
Twitter
EmailClick to copy linkLink copiedCitePark, Sang Myun (2024). The analysis of composition and abundance of the raft proteome of microglia using a tandem mass tag (TMT)-based quantitative proteomic analysis. [Dataset]. https://data.niaid.nih.gov/resources?id=zenodo_5211082Dataset updatedJul 18, 2024Dataset provided byPark, Sang Myun
Park, Soo Jung
Jou, Ilo
Woo, Joo Hong
Joe, Eun-hyeLicenseAttribution 4.0 (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/
License information was derived automaticallyDescriptionTo determine the proteins in the membrane raft, we used the TMT-labeling and nano-liquid chromatography mass spectrometry (nano-LC-MS/MS) analysis by Creative Proteomics (NY, USA; https://www.creative-proteomics.com/). Rat primary microglia were treated with IL-6 (25 ng/ml) for 15 min. Membrane rafts were obtained by flotation assay. Samples were prepared from three independent experiments. Proteins in equal volumes of raft fractions were digested with trypsin, desalted, and labeled with a TMT reagent (Thermo Fisher Science). The TMT-labeled peptides were fractionated and analyzed by nano-LC-MS/MS. The resulting MS/MS data were analyzed and searched against the rat protein database using Proteome Discoverer 2.1.
Selected Hits from Mass Spectrometry Assays from Lipid Rafts Proteins.
- plos.figshare.com
xlsUpdated Jun 1, 2023ShareFacebookTwitterEmailClick to copy linkLink copiedCiteAndrés B. Lantos; Giannina Carlevaro; Beatriz Araoz; Pablo Ruiz Diaz; María de los Milagros Camara; Carlos A. Buscaglia; Mariano Bossi; Hai Yu; Xi Chen; Carolyn R. Bertozzi; Juan Mucci; Oscar Campetella (2023). Selected Hits from Mass Spectrometry Assays from Lipid Rafts Proteins. [Dataset]. http://doi.org/10.1371/journal.ppat.1005559.t001xlsAvailable download formatsUnique identifierhttps://doi.org/10.1371/journal.ppat.1005559.t001Dataset updatedJun 1, 2023AuthorsAndrés B. Lantos; Giannina Carlevaro; Beatriz Araoz; Pablo Ruiz Diaz; María de los Milagros Camara; Carlos A. Buscaglia; Mariano Bossi; Hai Yu; Xi Chen; Carolyn R. Bertozzi; Juan Mucci; Oscar CampetellaLicenseAttribution 4.0 (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/
License information was derived automaticallyDescriptionSelected Hits from Mass Spectrometry Assays from Lipid Rafts Proteins.
Not seeing a result you expected?
Learn how you can add new datasets to our index.
FacebookTwitterEmailThe analysis of composition and abundance of the raft proteome of microglia using a tandem mass tag (TMT)-based quantitative proteomic analysis.
Park, Soo Jung
Jou, Ilo
Woo, Joo Hong
Joe, Eun-hye
Attribution 4.0 (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/
License information was derived automatically
To determine the proteins in the membrane raft, we used the TMT-labeling and nano-liquid chromatography mass spectrometry (nano-LC-MS/MS) analysis by Creative Proteomics (NY, USA; https://www.creative-proteomics.com/). Rat primary microglia were treated with IL-6 (25 ng/ml) for 15 min. Membrane rafts were obtained by flotation assay. Samples were prepared from three independent experiments. Proteins in equal volumes of raft fractions were digested with trypsin, desalted, and labeled with a TMT reagent (Thermo Fisher Science). The TMT-labeled peptides were fractionated and analyzed by nano-LC-MS/MS. The resulting MS/MS data were analyzed and searched against the rat protein database using Proteome Discoverer 2.1.