Hybridization among taxa impacts a variety of evolutionary processes from adaptation to extinction. We seek to understand both patterns of hybridization across taxa and the evolutionary and ecological forces driving those patterns. To this end, we use whole-genome low-coverage sequencing of 458 wild-grown and 1565 offspring individuals to characterize the structure, stability, and mating dynamics of admixed populations of Mimulus guttatus and Mimulus nasutus across a decade of sampling. In three streams, admixed genomes are common and a M. nasutus organellar haplotype is fixed in M. guttatus, but new hybridization events are rare. Admixture is strongly unidirectional, but each stream has a unique distribution of ancestry proportions. In one stream, three distinct cohorts of admixed ancestry are spatially structured at ~20-50m resolution and stable across years. Mating system provides almost complete isolation of M. nasutus from both M. guttatus and admixed cohorts, and is a partial barr..., For full collection and processing details, see the associated manuscript and the analysis code available at https://github.com/mfarnitano/CAC_popgen. Along three streams in the Columbia River Gorge area, Washington, USA, 0.5x0.5m square plots were laid down where Mimulus species were growing, during flowering seasons (April through June) of 2012, 2019, 2021, and 2022 (note that not all streams were sampled every year, for details see the associated manuscript). At each plot for the years 2012, 2019, and 2022, phenological data was recorded: the total number of open flowers within each plot was counted every few days for the duration of the flowering season. In addition, leaf or bud tissue was collected for sequencing from wild samples of Mimulus guttatus, Mimulus nasutus, and admixed individuals, as well as the allopolyploid species Mimulus sookensis, growing in these streams during the years 2019, 2021, and 2022. For a subset of these wild samples in 2019 and 2022..., , # Data from: Fluctuating reproductive isolation and stable ancestry structure in a fine-scaled mosaic of hybridizing Mimulus monkeyflowers

This dataset provides sample-level and plot-level metadata and processed data related to the analysis of admixture structure and reproductive isolation across space and time within a hybridizing population of Mimulus guttatus and Mimulus nasutus monkeyflowers.

Plot-level observations and sample collections took place during 2019, 2021, and 2022 flowering seasons (April to June) at two sites near the Columbia River Gorge, Washington, USA, named Catherine Creek (CAC) and Little Maui (LM). Tissue was collected from wild samples for sequencing. For some wild samples, offspring seeds were also collected and germinated in the UGA Botany greenhouses for tissue collection and sequencing. Field data and collections were done by Andrea Sweigart and Keith Karoly. Offspring processing, tissue preparation, sequencing, and analysis was done primarily by Matthew ...

Infaunal marine invertebrates were collected from inside and outside of patches of white bacterial mats from several sites in the Windmill Islands, Antarctica, around Casey station during the 2006-07 summer. Samples were collected from McGrady Cove inner and outer, the tide gauge near the Casey wharf, Stevenson's Cove and Brown Bay inner. Sediment cores of 10cm depth and 5cm diameter were collected by divers using a PVC corer from inside (4 cores) and outside (4 cores) each bacterial patch. The size of each patch varied from site to site. Cores were sieved at 500 microns and the extracted fauna preserved in 4 percent neutral buffered formalin. All fauna were counted and identified to species where possible or assigned to morphospecies based on previous infaunal sampling around Casey.

An excel spreadsheet is available for download at the URL given below. The spreadsheet does not represent the complete dataset, and is only the bacterial mat infauna data.

Regarding the infauna dataset:

• in - in the mat or patch of bacteria and out is in the "normal" sediment surrounding the patch without evidence of any bacterial mat presence.
• Patch numbers were allocated to ensure there was no confusion between patches in the same area.
• Fauna names are our identification codes for each species. Some we have confirmed identifications for, some not. Species names, where we have them and as we get them, are listed against these codes in the Casey marine soft-sediment fauna identification guide.

This work was completed as part of ASAC 2201 (ASAC_2201).