The National Health and Nutrition Examination Surveys (NHANES) is a program of studies designed to assess the health and nutritional status of adults and children in the United States. The NHANES combines personal interviews and physical examinations, which focus on different population groups or health topics. These surveys have been conducted by the National Center for Health Statistics (NCHS) on a periodic basis from 1971 to 1994. In 1999 the NHANES became a continuous program with a changing focus on a variety of health and nutrition measurements which were designed to meet current and emerging concerns. The surveys examine a nationally representative sample of approximately 5,000 persons each year. These persons are located in counties across the United States, 15 of which are visited each year. The 1999-2000 NHANES contains data for 9,965 individuals (and MEC examined sample size of 9,282) of all ages. Many questions that were asked in NHANES II, 1976-1980, Hispanic HANES 1982-1984, and NHANES III, 1988-1994, were combined with new questions in the NHANES 1999-2000. The 1999-2000 NHANES collected data on the prevalence of selected chronic conditions and diseases in the population and estimates for previously undiagnosed conditions, as well as those known to and reported by respondents. Risk factors, those aspects of a person's lifestyle, constitution, heredity, or environment that may increase the chances of developing a certain disease or condition, were examined. Data on smoking, alcohol consumption, sexual practices, drug use, physical fitness and activity, weight, and dietary intake were collected. Information on certain aspects of reproductive health, such as use of oral contraceptives and breastfeeding practices, were also collected. The interview includes demographic, socioeconomic, dietary, and health-related questions. The examination component consists of medical, dental, and physiological measurements, as well as laboratory tests. Demographic data file variables are grouped into three broad categories: (1) Status Variables: Provide core information on the survey participant. Examples of the core variables include interview status, examination status, and sequence number. (Sequence number is a unique ID assigned to each sample person and is required to match the information on this demographic file to the rest of the NHANES 1999-2000 data). (2) Recoded Demographic Variables: The variables include age (age in months for persons through age 19 years, 11 months; age in years for 1-84 year olds, and a top-coded age group of 85+ years), gender, a race/ethnicity variable, an education variable (high school, and more than high school education), country of birth (United States, Mexico, or other foreign born), and pregnancy status variable. Some of the groupings were made due to limited sample sizes for the two-year dataset. (3) Interview and Examination Sample Weight Variables: Sample weights are available for analyzing NHANES 1999-2000 data. For a complete listing of survey contents for all years of the NHANES see the document -- Survey Content -- NHANES 1999-2010.

Poxviruses are large DNA viruses infecting humans and animals. Vaccinia virus (VACV) has been applied as a live vaccine for immunization against smallpox, which was eradicated by 1980 as a result of worldwide vaccination. VACV is the prototype of poxviruses in the investigation of the molecular pathogenesis of the virus. Short-read sequencing methods have revolutionized transcriptomics; but, they are not efficient in distinguishing between the RNA isoforms and transcript overlaps. Long-read sequencing (LRS) is much better suited to solve these problems. Despite the scientific relevance of VACV, no LRS data have been generated for the viral transcriptome so far. For the deep characterization of the VACV RNA profile, various LRS platforms and library preparation approaches were applied. The raw reads were mapped to the VACV reference genome and also to the host (Chlorocebus sabaeus) genome. In this study, we applied the Pacific Biosciences RSII and Sequel platforms, which altogether resulted in 937,531 mapped reads of inserts (1.42 Gb), while we obtained 2,160,348 aligned reads (1.75 Gb) from the different library preparation methods, using the MinION device from Oxford Nanopore Technologies. By applying cutting-edge technologies, we were able to generate a large dataset that can serve as a valuable resource for the investigation of the dynamic VACV transcriptome, the virus-host interactions and the RNA base modifications. These data can provide useful information for novel gene annotations in the VACV genome. Our dataset can also be applied for analyzing the currently available LRS platforms, library preparation methods and bioinformatics pipelines.

Pakistan PK: Immunization: DPT: % of Children Aged 12-23 Months data was reported at 72.000 % in 2016. This stayed constant from the previous number of 72.000 % for 2015. Pakistan PK: Immunization: DPT: % of Children Aged 12-23 Months data is updated yearly, averaging 58.000 % from Dec 1980 (Median) to 2016, with 37 observations. The data reached an all-time high of 82.000 % in 2010 and a record low of 2.000 % in 1980. Pakistan PK: Immunization: DPT: % of Children Aged 12-23 Months data remains active status in CEIC and is reported by World Bank. The data is categorized under Global Database’s Pakistan – Table PK.World Bank: Health Statistics. Child immunization, DPT, measures the percentage of children ages 12-23 months who received DPT vaccinations before 12 months or at any time before the survey. A child is considered adequately immunized against diphtheria, pertussis (or whooping cough), and tetanus (DPT) after receiving three doses of vaccine.; ; WHO and UNICEF (http://www.who.int/immunization/monitoring_surveillance/en/).; Weighted average;