4 datasets found
  1. The analytic procedure of the example RT-qPCR data using SASqPCR.

    • plos.figshare.com
    xls
    Updated May 30, 2023
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    Daijun Ling (2023). The analytic procedure of the example RT-qPCR data using SASqPCR. [Dataset]. http://doi.org/10.1371/journal.pone.0029788.t002
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    xlsAvailable download formats
    Dataset updated
    May 30, 2023
    Dataset provided by
    PLOShttp://plos.org/
    Authors
    Daijun Ling
    License

    Attribution 4.0 (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/
    License information was derived automatically

    Description

    *The folder “X:\qPCR” in code #1, #2 and #3 needs to be changed to the appropriate path and filename so that SAS software can successfully access it. Input names of genes and samples must exactly match those in the original dataset. Please note that it is possible but not necessary to use the same Excel file to save the raw Ct data and exported results.

  2. i

    Season Agriculture Survey 2019 - Rwanda

    • catalog.ihsn.org
    Updated Aug 2, 2023
    + more versions
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    National Institute of Statistics of Rwanda (2023). Season Agriculture Survey 2019 - Rwanda [Dataset]. https://catalog.ihsn.org/catalog/study/RWA_2019_SAS_v01_M
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    Dataset updated
    Aug 2, 2023
    Dataset authored and provided by
    National Institute of Statistics of Rwanda
    Time period covered
    2018 - 2019
    Area covered
    Rwanda
    Description

    Abstract

    The main objective of the Seasonal Agricultural Survey is to provide timely, accurate, reliable and comprehensive agricultural statistics that describe the structure of agriculture in Rwanda mainly in terms of land use, crop area, yield and crop production to monitor current agricultural and food supply conditions and to facilitate evidence-based decision making for the development of the agricultural sector.

    In this regard, the National Institute of Statistics of Rwanda conducted the Seasonal Agriculture Survey (SAS) from September 2018 to august 2019 to gather up-to-date information for monitoring progress on agriculture programs and policies. This 2019 SAS covered Main agricultural seasons are Season A (which starts from September to February of the following year) and Season B (which starts from March to June). Season C is the small agricultural season mainly for vegetables and sweet potato grown in swamps and Irish potato grown in volcanic agro-ecological zone and provides data on farm characteristics (area, yield and production), agricultural practices, agricultural inputs and use of crop production

    Geographic coverage

    National coverage allowing district-level estimation of key indicators

    Analysis unit

    This seasonal agriculture survey focused on the following units of analysis: Small scale agricultural farms and large scale farms

    Universe

    The SAS 2019 targeted potential agricultural land and large scale farmers

    Kind of data

    Sample survey data [ssd]

    Sampling procedure

    Out of 10 strata, only 4 are considered to represent the country land potential for agriculture, and they cover the total area of 1,787,571.2 hectares (ha). Those strata are: 1.0 (tea plantations), 1.1 (intensive agriculture land on hillsides), 2.0 (intensive agriculture land in marshlands) and 3.0 (rangelands). The remainder of land use strata represents all the non-agricultural land in Rwanda. Stratum 1.0, which represents tea plantations, is assumed to be well monitored through administrative records by the National Agriculture Export Board (NAEB), an institution whose main mission is to promote the agriculture export commodities. Thus, SAS is conducted on 3 strata (1.1; 2.0 & 3.0) to cover other major crops. Within district, the agriculture strata (1.1, 2.0 & 3.0) were divided into larger sampling units called first-step or primary sampling units (PSUs) (as shown in Figure 2). Strata 1.1 and 2.0 were divided into PSUs of around 100 ha while stratum 3.0 was divided into PSUs of around 500 ha. After sample size determination, a sample of PSUs was done by systematic sampling method with probability proportional to size, then a given number of PSUs to be selected for each stratum, was assigned in every district. In 2019, the 2018 SAS sample of 780 segments has been kept the same for SAS 2019 in Season A and B.

    At first stage, 780 PSUs sampled countrywide were proportionally allocated in different levels of stratification (Hill side, marshland and rangeland strata) for 30 districts of Rwanda, to allow publication of results at district level. Sampled PSUs in each stratum were systematically selected from the frame with probability of selection proportional to the size of the PSU.

    At the second stage 780 sampled PSUs were divided into secondary sampling units (SSUs) also called segments. Each segment is estimated to be around 10 ha for strata 1.1 and 2.0 and 50 ha for stratum 3.0 (as shown in Figure 3). For each PSU, only one SSU is selected by random sampling method without replacement. This is why for 2019 5 SAS season A and B, the same number of 780 SSUs was selected. In addition to this, a list frame of large-scale farmers (LSF), with at least 10 hectares of agricultural holdings, was done to complement the area frame just to cover crops mostly grown by large scale farmers and that cannot be easily covered in area frame

    At the last sampling stage, in strata 1.1 and 2.0 each segment of an average size of 10 ha (100,000 Square meters) has been divided into around 1,000 grids squares of 100 Sq. meters each, while for stratum 3.0 around 5,000 grids squares of 100 Sq. meters each have been divided. A point was placed at the center of every grid square and named a grid point (A grid point is a geographical location at the center of every grid square). A random sample of 5% of the total grid points were selected in each segment of strata 1.1 and 2.0 whereas a random sample of 2% of total grid points was selected in each segment of stratum 3.0. Grids points are reporting units within a segment, where enumerators go to every grid point, locate and delineate the plots in which the grid falls, and collect records of land use and related information. The recorded information represents the characteristics of the whole segment which are extrapolated to the stratum level and hence the combination of strata within each district provides district area related statistics.

    Mode of data collection

    Face-to-face [f2f]

    Research instrument

    There were two types of questionnaires used for this survey namely screening questionnaire and plot questionnaire. A Screening questionnaire was used to collect information that enabled identification of a plot and its land use using the plot questionnaire. For point-sampling, the plot questionnaire is concerned with the collection of data on characteristics of crop identification, crop production and use of production, inputs (seeds, fertilizers and pesticides), agricultural practices and land tenure. All the surveys questionnaires used were published in English

    Cleaning operations

    The CAPI method of data collection allows the enumerators in the field to collect and enter data with their tablets and then synchronize to the server at headquarters where data are received by NISR staff, checked for consistency at NISR and thereafter transmitted to analysts for tabulation using STATA software, and reporting using office Excel and word as well.

    Response rate

    Data collection was done in 780 segments and 222 large scale farmers holdings for Season A, whereas in Season C data was collected in 232 segments, response rate was 100% of the sample

  3. f

    Rwanda Seasonal Agriculture Survey 2016 - Rwanda

    • microdata.fao.org
    Updated Jul 10, 2019
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    National Institute of Statistics of Rwanda (2019). Rwanda Seasonal Agriculture Survey 2016 - Rwanda [Dataset]. https://microdata.fao.org/index.php/catalog/867
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    Dataset updated
    Jul 10, 2019
    Dataset authored and provided by
    National Institute of Statistics of Rwanda
    Time period covered
    2015 - 2016
    Area covered
    Rwanda
    Description

    Abstract

    The main objective of the new agricultural statistics program is to provide timely, accurate, credible and comprehensive agricultural statistics to describe the structure of agriculture in Rwanda in terms of land use, crop production and livestock; which can be used for food and agriculture policy formulation and planning, and for the compilation of national accounts statistics.

    In this regard, the National Institute of Statistics of Rwanda (NISR) conducted the Seasonal Agriculture Survey (SAS) from November 2015 to October 2016 to gather up-to-date information for monitoring progress on agriculture programs and policies in Rwanda, including the Second Economic Development and Poverty Reduction Strategy (EDPRS II) and Vision 2020. This 2016 RSAS covered three agricultural seasons (A, B and C) and provides data on background characteristics of the agricultural operators, farm characteristics (area, yield and production), agricultural practices, agricultural equipments, use of crop production by agricultural operators and by large scale farmers.

    Geographic coverage

    National coverage

    Analysis unit

    Agricultural holdings

    Universe

    The 2016 RSAS targeted agricultural operators and large scale farmers operating in Rwanda.

    Kind of data

    Sample survey data [ssd]

    Sampling procedure

    The Seasonal Agriculture Survey (SAS) sample is composed of two categories of respondents: agricultural operators1 and large-scale farmers (LSF).

    For the 2016 SAS, NISR used as the sampling method a dual frame sampling design combining selected area frame sample3 segments and a list of large-scale farmers.

    NISR used also imagery from RNRA with a very high resolution of 25 centimeters to divide the total land of the country into twelve strata. A total number of 540 segments were spread throughout the country as coverage of the survey with 25,346 and 23,286 agricultural operators in Season A and Season B respectively. From these numbers of agricultural operators, sub-samples were selected during the second phases of Seasons A and B.

    It is important to note that in each of agricultural season A and B, data collection was undertaken in two phases. Phase I was mainly used to collect data on demographic and social characteristics of interviewees, area under crops, crops planted, rainfall, livestock, etc. Phase II was mainly devoted to the collection of data on yield and production of crops.

    Phase I serves at collecting data on area under different types of crops in the screening process, whereas the Phase II is mainly devoted to the collection of data on demographic, social characteristics of interviewees, together with yields of the different crops produced. Enumerated large-scale farmers (LSF) were 558 in both 2015 Season A and B. The LSF were engaged in either crop farming activities only, livestock farming activities only, or both crop and livestock farming activities.

    Agricultural operators are the small scale farmers within the sample segments. Every selected segment was firstly screened using the appropriate materials such as the segment maps, GIS devices and the screening form. Using these devices, the enumerators accounted for every plot inside the sample segments. All Tracts6 were classified as either agricultural (cultivated land, pasture, and fallow land) or non-agricultural land (water, forests, roads, rocky and bare soils, and buildings).

    During Phase I, a complete enumeration of all farmers having agricultural land and operating within the 540 selected segments was undertaken and a total of 25,495 and 24,911 agricultural operators were enumerated respectively in Seasons A and B. Season C considered only 152 segments, involving 3,445 agricultural operators.

    In phase II, 50% of the large-scale farmers were undertaking crop farming activities only and 50% of the large-scale farmers were undertaking both crop and livestock farming and were selected for interview. A sample of 199 and 194 large-scale farmers were interviewed in Seasons A and B, respectively, using a farm questionnaire.

    From the agricultural operators enumerated in the sample segments during Phase I, a sample of the agricultural operators was designed for Phase II as follows: 5,502 for Season A, 5,337 for Season B and 644 for Season C. The method of probability proportional to size (PPS) sampling at the national level was used. Furthermore, the total number of enumerated large-scale farmers was 774 in 2016 Season A and 622 in Season B.

    The Season C considered 152 segments counting 8,987 agricultural operators from which 963 agricultural operators were selected for survey interviews.

    Mode of data collection

    Face-to-face paper [f2f]

    Research instrument

    There were two types of questionnaires used for this survey namely Screening questionnaire and farm questionnaires.

    A Screening Questionnaire was used to collect information that enabled identification of an Agricultural Operator or Large Scale Farmer and his or her land use.
    Farm questionnaires were of two types: a) Phase I Farm Questionnaire was used to collect data on characteristics of Agricultural Operators, crop identification and area, inputs (seeds, fertilizers, labor, …) for Agricultural Operators and large scale farmers. b) Phase 2 Farm questionnaire was used in the collection of data on crop production and use of production.

    It is important to mention that all these Farm Questionnaires were subjected to two/three rounds of data quality checking. The first round was conducted by the enumerator and the second round was conducted by the team leader to check if questionnaires had been well completed by enumerators. For season C, after screening, an interview was conducted for each selected tract/Agricultural Operator using one consolidated Farm questionnaire. All the surveys questionnaires used were published in both English and Kinyarwanda languages.

    Cleaning operations

    Data editing took place at different stage. Firstly, the filled questionnaires were repatriated at NISR for office editing and coding before data entry started. Data entry of the completed and checked questionnaires was undertaken at the NISR office by 20 staff trained in using the CSPro software. To ensure appropriate matching of data in the completed questionnaires and plot area measurements from the GIS unit, a LOOKUP file was integrated in the CSPro data entry program to confirm the identification of each agricultural operator or LSF before starting data entry. Thereafter, data were entered in computers, edited and summarized in tables using SPSS and Excel.

    Response rate

    The response rate for Seasonal Agriculture Survey is 98%.

    Data appraisal

    All Farm questionnaires were subjected to two/three rounds of data quality checking. The first round was conducted by the enumerator and the second round was conducted by the team leader to check if questionnaires had been well completed by enumerators. And in most cases, questionnaires completed by one enumerator were peer-reviewed by another enumerator before being checked by the Team leader.

  4. g

    ABC News/Washington Post Drug Poll, February 1997 - Version 2

    • search.gesis.org
    Updated Feb 21, 1997
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    GESIS search (1997). ABC News/Washington Post Drug Poll, February 1997 - Version 2 [Dataset]. http://doi.org/10.3886/ICPSR02175.v2
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    Dataset updated
    Feb 21, 1997
    Dataset provided by
    GESIS search
    ICPSR - Interuniversity Consortium for Political and Social Research
    License

    https://search.gesis.org/research_data/datasearch-httpwww-da-ra-deoaip--oaioai-da-ra-de454978https://search.gesis.org/research_data/datasearch-httpwww-da-ra-deoaip--oaioai-da-ra-de454978

    Description

    Abstract (en): This special topic poll, conducted February 20-24, 1997, solicited responses from parents and their teenage children, aged 12-17, on the topic of illegal drug use among America's youth. One parent and one child from each household were asked a series of questions covering illegal drugs, violence in school, underage drinking, academic challenges, and parent-child communication. Respondents were asked to assess their understanding of the presence of drugs and drug users in their local schools, throughout the community, across the nation, among the teen's peer group, and within their own family. A series of topics covered the availability and effectiveness of school-sponsored anti-drug programs. Parents were asked how their possible past and present use and/or experimentation with marijuana and other illegal drugs, alcohol, and tobacco products influenced the manner in which they approached drug use with their own children. Teenage respondents were asked for their reaction to the use of drugs and alcohol by their friends, the seriousness of the contemporary drug problem, and whether they believed that their parents had used or experimented with illegal drugs. Other questions asked about teenage respondents' plans after high school and whether they attended a public or private school. Demographic variables for parental respondents included age, race, sex, education level, household income, political party affiliation, and type of residential area (e.g., urban or rural). Demographic variables for teenage respondents included age, race, sex, residential area, and grade level in school. The data contain a weight variable (WEIGHT) that should be used in analyzing the data. This poll consists of "standard" national representative samples of the adult population with sample balancing of sex, race, age, and education. The weight variable contains two implied decimal places, and applies only to the parental respondents. ICPSR data undergo a confidentiality review and are altered when necessary to limit the risk of disclosure. ICPSR also routinely creates ready-to-go data files along with setups in the major statistical software formats as well as standard codebooks to accompany the data. In addition to these procedures, ICPSR performed the following processing steps for this data collection: Created online analysis version with question text.. Persons aged 18 and over living in households with telephones in the contiguous 48 United States. Households were selected by random-digit dialing. Within households, the respondent selected was the adult living in the household who last had a birthday and who was home at the time of the interview. 2007-02-27 SAS, SPSS, and Stata setup files, and SAS and Stata supplemental files have been added to this data collection. Respondent names were removed from the data file and the CASEID variable was created for use with online analysis.2006-11-10 SAS, SPSS, and Stata setup files have been added to this data collection. telephone interview (1) The data available for download are not weighted and users will need to weight the data prior to analysis. (2) Original reports using these data may be found via the ABC News Web site. (3) According to the data collection instrument, code 3 in the variable P_EDUC also included respondents who answered that they had attended a technical college. (4) The CASEID variable was created for use with online analysis.

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Daijun Ling (2023). The analytic procedure of the example RT-qPCR data using SASqPCR. [Dataset]. http://doi.org/10.1371/journal.pone.0029788.t002
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The analytic procedure of the example RT-qPCR data using SASqPCR.

Related Article
Explore at:
xlsAvailable download formats
Dataset updated
May 30, 2023
Dataset provided by
PLOShttp://plos.org/
Authors
Daijun Ling
License

Attribution 4.0 (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/
License information was derived automatically

Description

*The folder “X:\qPCR” in code #1, #2 and #3 needs to be changed to the appropriate path and filename so that SAS software can successfully access it. Input names of genes and samples must exactly match those in the original dataset. Please note that it is possible but not necessary to use the same Excel file to save the raw Ct data and exported results.

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