TA

## Overview

TA is a dataset for object detection tasks - it contains Face Detection annotations for 2,448 images.

## Getting Started

You can download this dataset for use within your own projects, or fork it into a workspace on Roboflow to create your own model.

  ## License

  This dataset is available under the [Public Domain license](https://creativecommons.org/licenses/Public Domain).

Several members are annotated as being of the abortive phage resistance system, in which case the family would be acting as the toxin for a type IV toxin-antitoxin resistance system.

PIN domains are small protein domains identified by the presence of three strictly conserved acidic residues. Apart from these three residues, there is poor sequence conservation . PIN domains are found in eukaryotes, eubacteria and archaea. In eukaryotes they are ribonucleases involved in nonsense mediated mRNA decay and in processing of 18S ribosomal RNA . In prokaryotes, they are the toxic components of toxin-antitoxin (TA) systems, their toxicity arising by virtue of their ribonuclease activity. The PIN domain TA systems are now called VapBC TAs(virulence associated proteins), where VapB is the inhibitor and VapC, the PIN-domain ribonuclease toxin .

TA 1

## Overview

TA 1 is a dataset for object detection tasks - it contains Mobil Motor PlatNomor annotations for 939 images.

## Getting Started

You can download this dataset for use within your own projects, or fork it into a workspace on Roboflow to create your own model.

  ## License

  This dataset is available under the [Public Domain license](https://creativecommons.org/licenses/Public Domain).

Explore the historical Whois records related to ta-ta-mi.org (Domain). Get insights into ownership history and changes over time.

Protein-Protein, Genetic, and Chemical Interactions for Chartron JW (2012):Structures of the Sgt2/SGTA Dimerization Domain with the Get5/UBL4A UBL Domain Reveal an Interaction that Forms a Conserved Dynamic Interface. curated by BioGRID (https://thebiogrid.org); ABSTRACT: In the cytoplasm, the correct delivery of membrane proteins is an essential and highly regulated process. The posttranslational targeting of the important tail-anchor membrane (TA) proteins has recently been under intense investigation. A specialized pathway, called the guided entry of TA proteins (GET) pathway in yeast and the transmembrane domain recognition complex (TRC) pathway in vertebrates, recognizes endoplasmic-reticulum-targeted TA proteins and delivers them through a complex series of handoffs. An early step is the formation of a complex between Sgt2/SGTA, a cochaperone with a presumed ubiquitin-like-binding domain (UBD), and Get5/UBL4A, a ubiquitin-like domain (UBL)-containing protein. We structurally characterize this UBD/UBL interaction for both yeast and human proteins. This characterization is supported by biophysical studies that demonstrate that complex formation is mediated by electrostatics, generating an interface that has high-affinity with rapid kinetics. In total, this work provides a refined model of the interplay of Sgt2 homologs in TA targeting.

Proteins are listed according to their PlasmoDB ID (https://plasmodb.org) and descriptions. Attributes of each protein include the transmembrane domain (TMD) sequence and its calculated hydrophobicity score (according to GRAVY calculator; http://www.gravy-calculator.de), the C-terminus sequence (CTS) and the net charge of the CTS. Predicted organellar localizations, as designated in this study, are also indicated. The complete list of TA proteins is shown in S1Table.

PIN (PilT N terminus) domain of the Saccharomyces cerevisiae exosome subunit Rrp44 (Ribosomal RNA-processing protein 44 or Protein Dis3 homolog) and other similar eukaryotic homologs are included in this family. The eukaryotic exosome is a conserved macromolecular complex responsible for many RNA-processing and RNA-degradation reactions. It is composed of nine core subunits that directly binds Rrp44. The Rrp44 nuclease is the catalytic subunit of the exosome and has endonuclease activity in the PIN domain and an exoribonuclease activity in its RNase II-like region. Rrp44 binding to the exosome is mediated mainly by the PIN domain and by subunits Rrp41-Rrp45, and binding predictions indicate that the PIN domain active site is positioned on the outer surface of the exosome. This subgroup belongs to the VapC (virulence-associated protein C)-like family of the PIN domain nuclease superfamily. VapC is the PIN-domain ribonuclease toxin from prokaryotic VapBC toxin-antitoxin (TA) systems. VapB is a transcription factor-like protein antitoxin acting as an inhibitor. Other members of the VapC-like nuclease family include FitB toxin of the FitAB TA system, eukaryotic ribonucleases such as Smg6, ribosome assembly factor NOB1, exosome subunit Rrp44 endoribonuclease and rRNA-processing protein Fcf1. The structural properties of the PIN (PilT N terminus) domain indicate its active center, consisting of three highly conserved catalytic residues which coordinate metal ions, in some members, additional metal coordinating residues can be found. Some members of the superfamily lack several of these key catalytic residues. PIN domains within this subgroup contain four of these residues which cluster at the C-terminal end of the beta-sheet and form a negatively charged pocket near the center of the molecule. Recombinant Rrp44 was shown to possess manganese-dependent endonuclease activity in vitro that was abolished by point mutations in these putative metal binding residues of its PIN domain. The PIN active site is geometrically similar in the active center of structure-specific 5' nucleases, PIN-domain ribonucleases of eukaryotic rRNA editing proteins, and bacterial toxins of toxin-antitoxin (TA) operons.

Explore the historical Whois records related to ta.ai (Domain). Get insights into ownership history and changes over time.

TA 2

## Overview

TA 2 is a dataset for object detection tasks - it contains Motor Mobil PlatNumor annotations for 2,786 images.

## Getting Started

You can download this dataset for use within your own projects, or fork it into a workspace on Roboflow to create your own model.

  ## License

  This dataset is available under the [Public Domain license](https://creativecommons.org/licenses/Public Domain).

Project TA

## Overview

Project TA is a dataset for object detection tasks - it contains Poeple annotations for 808 images.

## Getting Started

You can download this dataset for use within your own projects, or fork it into a workspace on Roboflow to create your own model.

  ## License

  This dataset is available under the [Public Domain license](https://creativecommons.org/licenses/Public Domain).

Affinity reagents of high affinity and specificity are very useful for studying the subcellular locations and quantities of individual proteins. To generate high-quality affinity reagents for human Lyn tyrosine kinase, a phage display library of fibronectin type III (FN3) monobodies was affinity selected with a recombinant form of the Lyn SH3 domain. While a highly specific monobody, TA8, was initially isolated, we chose to improve its affinity through directed evolution. A secondary library of 1.2 × 109 variants was constructed and screened by affinity selection, yielding three variants, two of which have affinities of ~ 40 nM, a 130-fold increase over the original TA8 monobody. One of the variants, 2H7, displayed high specificity to the Lyn SH3 domain, as shown by ELISA and probing arrays of 150 SH3 domains. Furthermore, the 2H7 monobody was able to pull down endogenous Lyn from a lysate of Burkitt's lymphoma cells, thereby demonstrating its utility as an affinity reagent for detecting Lyn in a complex biological mixture.

Explore the historical Whois records related to ta.org.pt (Domain). Get insights into ownership history and changes over time.

Results of the domain search with Pfam, using the amino acid sequence of each protein.

Conserved domains and homologous VapB proteins were identified by homology searches using pBLAST. Predicted molecular weights (MWs) of VapBC heterodimers were calculated by ProtParam [28]. Molecular weights of VapBC complexes were calculated by calibration of an S200 analytical gel filtration column (SEC). Stoichiometry of the VapBC complex was determined by division of the molecular weight from gel filtration analysis with the predicted molecular weight of a VapBC heterodimer.

This archive contains the spatial frequency domain Mueller matrix data associated with the paper J. Chue-Sang, M. Litorja, A. M. Goldfain, and T. A. Germer, "Spatial Frequency domain Mueller matrix imaging," J. Biomedical Optics 27(12), 126003 (2022). The paper shows a subset of the data included in this archive. A Python script, analyze.py, is provided to assist the user in reading the data. The script can be run without any arguments from the top folder and will generate all the figures included in this archive. The script requires Python 3.6 and Matplotlib 3.4.A MATLAB script, analyze.m, is also provided.

Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum (ER) (PubMed:21444755, PubMed:23041287, PubMed:24392163, PubMed:27226539). Together with CAMLG/GET2, acts as a membrane receptor for soluble GET3/TRC40, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol (PubMed:21444755, PubMed:23041287, PubMed:24392163, PubMed:27226539). Required to ensure correct topology and ER insertion of CAMLG (PubMed:31417168, PubMed:32187542)

Cut sites determined using the improved in-house software on collected MALDI-TOF MS data.

BackgroundDifferentiation of one life-cycle stage to the next is critical for survival and transmission of apicomplexan parasites. A number of studies have shown that stage differentiation is a stochastic process and is associated with a point that commits the cell to a change over in the pattern of gene expression. Studies on differentiation to merozoite production (merogony) in T. annulata postulated that commitment involves a concentration threshold of DNA binding proteins and an auto-regulatory loop.Principal FindingsIn this study ApiAP2 DNA binding proteins that show changes in expression level during merogony of T. annulata have been identified. DNA motifs bound by orthologous domains in Plasmodium were found to be enriched in upstream regions of stage-regulated T. annulata genes and validated as targets for the T. annulata AP2 domains by electrophoretic mobility shift assay (EMSA). Two findings were of particular note: the gene in T. annulata encoding the orthologue of the ApiAP2 domain in the AP2-G factor that commits Plasmodium to gametocyte production, has an expression profile indicating involvement in transmission of T. annulata to the tick vector; genes encoding related domains that bind, or are predicted to bind, sequence motifs of the type 5'-(A)CACAC(A) are implicated in differential regulation of gene expression, with one gene (TA11145) likely to be preferentially up-regulated via auto-regulation as the cell progresses to merogony.ConclusionsWe postulate that the Theileria factor possessing the AP2 domain orthologous to that of Plasmodium AP2-G may regulate gametocytogenesis in a similar manner to AP2-G. In addition, paralogous ApiAP2 factors that recognise 5'-(A)CACAC(A) type motifs could operate in a competitive manner to promote reversible progression towards the point that commits the cell to undergo merogony. Factors possessing AP2 domains that bind (or are predicted to bind) this motif are present in the vector-borne genera Theileria, Babesia and Plasmodium, and other Apicomplexa; leading to the proposal that the mechanisms that control stage differentiation will show a degree of conservation.

• Entries for English-Spanish: Scientific research & mathematical sciences (906 entries), Geosciences (10,215), Computer science, electronics & telecommunications (70,580), Industry (47,578), Transport & Maintenance (12,291), Economy (145,572), Biological sciences (38,989), Communication & media (8,143), Chemical & physical sciences (27,467). * Entries for English-French-German-Spanish: Environment (36,658), Health (66,727), Agriculture & food (25,975), Construction & public works (8,429), Law & policy (56,578), Sports & Leisure (17,312) * Two specialized lexicons: Spanish-English and English-French-German without domain codes: electronics, telematics, law, taxes, customs, etc. (550,000 entries). * Two general lexicons: Spanish-English-French-German and Spanish-English-French-German-Portuguese-Italian (83,000 entries).This terminological database contains, for each domain, a sub-domain indication is given (from 2 sub-domains for Scientific research to 39 for Sports & leisure). Each entry consists of a definition, phraseological unit, abbreviation, usage information, grammatical labels. Format: ASCII