Groundwater wells are critical infrastructure that enable the monitoring, extraction, and use of groundwater, which has important implications for the environment, water security, and economic development. Despite the importance of wells, a unified database collecting and standardizing information on the characteristics and locations of these wells across the United States has been lacking. To bridge this gap, we have created a comprehensive database of groundwater well records collected from state and federal agencies, which we call the United States Groundwater Well Database (USGWD). Presented in both tabular form and as vector points, the USGWD comprises over 14.2 million well records with attributes such as well purpose, location, depth, and capacity for wells constructed as far back as 1763 to 2023. Rigorous cross-verification steps have been applied to ensure the accuracy of the data. The USGWD stands as a valuable tool for improving our understanding of how groundwater is accessed and managed across various regions and sectors within the United States.