The potential use of bacteria for developing novel vector control approaches has awakened new interests in the study of the microbiota associated with vector species. To set a baseline for future malaria research, a high-throughput sequencing of the bacterial 16S ribosomal gene V3-V4 region was used to profile the microbiota associated with late-instar larvae, newly emerged females, and wild-caught females of a sylvan Anopheles atroparvus population from a former malaria transmission area of Spain. Field-acquired microbiota was then assessed in non-blood-fed laboratory-reared females from the second, sixth, and 10th generations. Diversity analyses revealed that bacterial communities varied and clustered differently according to origin with sylvan larvae and newly emerged females distributing closer to laboratory-reared females than to their field counterparts. Inter-sample variation was mostly observed throughout the different developmental stages in the sylvan population. Larvae harbored the most diverse bacterial communities; wild-caught females, the poorest. In the transition from the sylvan environment to the first time point of laboratory breeding, a significant increase in diversity was observed, although this did decline under laboratory conditions. Despite diversity differences between wild-caught and laboratory-reared females, a substantial fraction of the bacterial communities was transferred through transstadial transmission and these persisted over 10 laboratory generations. Differentially abundant bacteria were mostly identified between breeding water and late-instar larvae, and in the transition from wild-caught to laboratory-reared females from the second generation. Our findings confirmed the key role of the breeding environment in shaping the microbiota of An. atroparvus. Gram-negative bacteria governed the microbiota of An. atroparvus with the prevalence of proteobacteria. Pantoea, Thorsellia, Serratia, Asaia, and Pseudomonas dominating the microbiota associated with wild-caught females, with the latter two governing the communities of laboratory-reared females. A core microbiota was identified with Pseudomonas and Serratia being the most abundant core genera shared by all sylvan and laboratory specimens. Overall, understanding the microbiota composition of An. atroparvus and how this varies throughout the mosquito life cycle and laboratory colonization paves the way when selecting potential bacterial candidates for use in microbiota-based intervention strategies against mosquito vectors, thereby improving our knowledge of laboratory-reared An. atroparvus mosquitoes for research purposes.

Glyphosate is the world's most widely used herbicide. The commercial success of this molecule is due to its non-selectivity and its action, which would supposedly target specific biosynthetic pathways found mainly in plants. Multiple studies have however provided evidence for high sensitivity of many non-target species to glyphosate and/or to formulations (glyphosate mixed with surfactants). This herbicide, found at significant levels in aquatic systems through surface runoffs, impacts life history traits and immune parameters of several aquatic invertebrates' species, including disease-vector mosquitoes. Mosquitoes, from hatching to emergence, are exposed to aquatic chemical contaminants. In this study, we first compared the toxicity of pure glyphosate to the toxicity of glyphosate-based formulations for the main vector of avian malaria in Europe, Culex pipiens mosquito. Then we evaluated, for the first time, how field realistic dose of glyphosate interacts with larval nutritional stress to alter mosquito life history traits and susceptibility to avian malaria parasite infection. Our results show that exposure of larvae to field-realistic doses of glyphosate, pure or in formulation, did not affect larval survival rate, adult size and female fecundity. One of our two experimental blocks showed, however, that exposure to glyphosate decreased development time and reduced mosquito infection probability by malaria parasite. Interestingly the effect on malaria infection was lost when the larvae were also subjected to a nutritional stress, probably due to a lower ingestion of glyphosate.

Vulnerable population identified by the nutritional status of children (weight for age and weight for height) as indicators for food security, in sample of households in West Africa study area. Data based on DHS and MICS surveys. In defining vulnerability, WFP (2009) and IFPRI (2012) have been followed and combined with indicators for food security with health indicators that signal vulnerability in a physical sense. IFPRI's Global Hunger Index uses three indicators to measure hunger: the number of adults being undernourished, the number of children that have low weight for age, and child mortality. Other classifications of food security use the variety of the diet as an indicator, combined with anthropometric data on children. However, in the DHS data there were no information available on child mortality, nor on dietary composition. Given these data limitations, data on nutritional status of women (Body Mass Index, BMI) for women and children (weight for age and weight for height) have been used as indicators for food security. These data were combined with data on morbidity among adults and children, specifically the occurrence of malaria, cough, and diarrhea. Combinations of indicators have led to a classification of households as being very vulnerable, vulnerable, nearly vulnerable and not vulnerable.

This data set was produced in the framework of the "Climate change predictions in Sub-Saharan Africa: impacts and adaptations (ClimAfrica)" project, Work Package 5 (WP5). More information on ClimAfrica project is provided in the Supplemental Information section of this metadata.

This study in WP5 aimed to identify, locate and characterize groups that are vulnerable for climate change conditions in two country clusters; one in West Africa (Benin, Burkina Faso, Côte d’Ivoire, Ghana, and Togo) and one in East Africa (Sudan, South Sudan and Uganda). Data used for the study include the Demographic and Health Surveys (DHS) , the Multi Indicator Cluster Survey (MICS) and the Afrobarometer surveys for the socio-economic variables and grid level data on agro-ecological and climatic conditions.

Data publication: 2013-08-01

Supplemental Information:

ClimAfrica was an international project funded by European Commission under the 7th Framework Programme (FP7) for the period 2010-2014. The ClimAfrica consortium was formed by 18 institutions, 9 from Europe, 8 from Africa, and the Food and Agriculture Organization of United Nations (FAO).

ClimAfrica was conceived to respond to the urgent international need for the most appropriate and up-to-date tools and methodologies to better understand and predict climate change, assess its impact on African ecosystems and population, and develop the correct adaptation strategies. Africa is probably the most vulnerable continent to climate change and climate variability and shows diverse range of agro-ecological and geographical features. Thus the impacts of climate change can be very high and can greatly differ across the continent, and even within countries.

The project focused on the following specific objectives:

1. Develop improved climate predictions on seasonal to decadal climatic scales, especially relevant to SSA;

2. Assess climate impacts in key sectors of SSA livelihood and economy, especially water resources and agriculture;

3. Evaluate the vulnerability of ecosystems and civil population to inter-annual variations and longer trends (10 years) in climate;

4. Suggest and analyse new suited adaptation strategies, focused on local needs;

5. Develop a new concept of 10 years monitoring and forecasting warning system, useful for food security, risk management and civil protection in SSA;

6. Analyse the economic impacts of climate change on agriculture and water resources in SSA and the cost-effectiveness of potential adaptation measures.

The work of ClimAfrica project was broken down into the following work packages (WPs) closely connected. All the activities described in WP1, WP2, WP3, WP4, WP5 consider the domain of the entire South Sahara Africa region. Only WP6 has a country specific (watershed) spatial scale where models validation and detailed processes analysis are carried out.

Contact points:

Metadata Contact: FAO-Data

Resource Contact: Lia van Wesenbeeck

Resource Contact: Ben Sonneveld

Resource constraints:

copyright

Online resources:

Weight for age <-3DS, % of population - Distribution in sample of households in West Africa

Weight for age -2SD --3SD, % of population - Distribution in sample of households in West Africa

Weight for age -2SD--0, % of population - Distribution in sample of households in West Africa

Weight for age >0SD, % of population - Distribution in sample of households in West Africa

A spatially explicit assessment of specific vulnerabilities of the food system due to climate change and the identification of their causes; Technical report

Scenarios of major production systems in Africa

Climafrica - Climate Change Predictions In Sub-Saharan Africa: Impacts And Adaptations

The potential use of bacteria for developing novel vector control approaches has awakened new interests in the study of the microbiota associated with vector species. To set a baseline for future malaria research, a high-throughput sequencing of the bacterial 16S ribosomal gene V3-V4 region was used to profile the microbiota associated with late-instar larvae, newly emerged females, and wild-caught females of a sylvan Anopheles atroparvus population from a former malaria transmission area of Spain. Field-acquired microbiota was then assessed in non-blood-fed laboratory-reared females from the second, sixth, and 10th generations. Diversity analyses revealed that bacterial communities varied and clustered differently according to origin with sylvan larvae and newly emerged females distributing closer to laboratory-reared females than to their field counterparts. Inter-sample variation was mostly observed throughout the different developmental stages in the sylvan population. Larvae harbored the most diverse bacterial communities; wild-caught females, the poorest. In the transition from the sylvan environment to the first time point of laboratory breeding, a significant increase in diversity was observed, although this did decline under laboratory conditions. Despite diversity differences between wild-caught and laboratory-reared females, a substantial fraction of the bacterial communities was transferred through transstadial transmission and these persisted over 10 laboratory generations. Differentially abundant bacteria were mostly identified between breeding water and late-instar larvae, and in the transition from wild-caught to laboratory-reared females from the second generation. Our findings confirmed the key role of the breeding environment in shaping the microbiota of An. atroparvus. Gram-negative bacteria governed the microbiota of An. atroparvus with the prevalence of proteobacteria. Pantoea, Thorsellia, Serratia, Asaia, and Pseudomonas dominating the microbiota associated with wild-caught females, with the latter two governing the communities of laboratory-reared females. A core microbiota was identified with Pseudomonas and Serratia being the most abundant core genera shared by all sylvan and laboratory specimens. Overall, understanding the microbiota composition of An. atroparvus and how this varies throughout the mosquito life cycle and laboratory colonization paves the way when selecting potential bacterial candidates for use in microbiota-based intervention strategies against mosquito vectors, thereby improving our knowledge of laboratory-reared An. atroparvus mosquitoes for research purposes.

Gene drive is an emerging biotechnology that can be used to bias the inheritance of a specific genetic trait in any population of sexually reproducing organisms. In Uganda, and in other African countries, there is interest in the possibility of using synthetic gene drives to reduce the population of the mosquito species Anopheles gambiae which is the primary transmitter of the malaria parasite plasmodium falciparum. This dataset contains transcripts of interviews conducted with a wide range of stakeholders; environmentalists, scientists, regulators and prospective implementers, on the topic of risk assessment for gene drive mosquitoes in Uganda.

Despite demands from Europe, USA and Africa for risk assessment of emerging technologies to be more inclusive, risk decisions remain highly contested, narrowly scientific and expert led. Risk assessment is a critical step in technology development yet we know very little about how to ‘open’ it up to stakeholders. The unique risks of gene drive technology make it an excellent example of this thorny problem, presenting a ‘constitutional moment’ to rethink international biotechnology governance. We address this problem through a case study of gene drive mosquitoes in Uganda to eradicate malaria, a disease with significant health and economic costs. Our team of social and natural scientists from the UK, USA and Uganda will develop new ways of thinking about and approaching risk assessment in collaboration with Ugandan stakeholders. Together, we will rethink traditional roles and distributions of expertise and allow Ugandans to take ownership of the oversight of the technology.